ECRG4 (NM_032411) Human Tagged ORF Clone

CAT#: RC206239

C2orf40 (Myc-DDK-tagged)-Human chromosome 2 open reading frame 40 (C2orf40)

Product Images

Western blot validation of overexpression lysate (Cat# LY403168) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206239 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ECRG4 protein (Cat# TP306239). The protein was produced from HEK293T cells transfected with ECRG4 cDNA clone (Cat# RC206239) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ECRG4
• Synonyms: C2orf40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206239 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCTCCCCCGCGCGGCCTGCTGTCCTGGCCCTGACCGGGCTGGCGCTGCTCCTGCTCCTGTGCT
  GGGGCCCAGGTGGCATAAGTGGAAATAAACTCAAGCTGATGCTTCAAAAACGAGAAGCACCTGTTCCAAC
  TAAGACTAAAGTGGCCGTTGATGAGAATAAAGCCAAAGAATTCCTTGGCAGCCTGAAGCGCCAGAAGCGG
  CAGCTGTGGGACCGGACTCGGCCCGAGGTGCAGCAGTGGTACCAGCAGTTTCTCTACATGGGCTTTGACG
  AAGCGAAATTTGAAGATGACATCACCTATTGGCTTAACAGAGATCGAAATGGACATGAATACTATGGCGA
  TTACTACCAACGTCACTATGATGAAGACTCTGCAATTGGTCCCCGGAGCCCCTACGGCTTTAGGCATGGA
  GCCAGCGTCAACTACGATGACTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206239 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAASPARPAVLALTGLALLLLLCWGPGGISGNKLKLMLQKREAPVPTKTKVAVDENKAKEFLGSLKRQKR
  QLWDRTRPEVQQWYQQFLYMGFDEAKFEDDITYWLNRDRNGHEYYGDYYQRHYDEDSAIGPRSPYGFRHG
  ASVNYDDY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032411
• ORF Size: 444 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032411.1, NM_032411.2, NP_115787.1
• RefSeq Size: 793 bp
• RefSeq ORF: 447 bp
• Locus ID: 84417
• Protein Families: Secreted Protein, Transmembrane
• MW: 17.2 kDa
• Gene Summary: Probable hormone that may induce senescence of oligodendrocyte and neural precursor cells, characterized by G1 arrest, RB1 dephosphorylation and accelerated CCND1 and CCND3 proteasomal degradation. [UniProtKB/Swiss-Prot Function]