SCRN1 (NM_014766) Human Tagged ORF Clone

CAT#: RC206268

SCRN1 (Myc-DDK-tagged)-Human secernin 1 (SCRN1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY428918) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227072 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SCRN1 protein (Cat# TP306268). The protein was produced from HEK293T cells transfected with SCRN1 cDNA clone (Cat# RC206268) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCRN1
• Synonyms: SES1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206268 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCAGCTCCTCCAAGTTACTGTTTTGTTGCCTTCCCTCCACGTGCTAAGGATGGTCTGGTGGTAT
  TTGGGAAAAATTCAGCCCGGCCCAGAGATGAAGTGCAAGAGGTTGTGTATTTCTCGGCTGCTGATCACGA
  ACCGGAGAGCAAGGTTGAGTGCACTTACATTTCAATCGACCAAGTTCCAAGGACCTATGCCATAATGATA
  AGCAGACCCGCCTGGCTCTGGGGAGCAGAAATGGGAGCCAATGAACATGGAGTGTGCATAGCCAATGAAG
  CCATCAACACCAGAGAGCCAGCTGCCGAGATAGAAGCCTTGCTGGGGATGGATCTGGTCAGGCTTGGTTT
  AGAAAGAGGGGAAACAGCTAAAGAAGCCTTAGATGTCATTGTCTCCTTGTTGGAAGAACATGGACAAGGT
  GGGAATTACTTTGAAGATGCAAACTCCTGCCACAGCTTCCAAAGTGCATATCTGATTGTGGATCGTGATG
  AAGCCTGGGTGCTCGAGACCATAGGGAAGTACTGGGCTGCCGAGAAAGTCACAGAGGGAGTGAGGTGCAT
  TTGCAGTCAGCTTTCGCTCACCACTAAGATGGATGCAGAGCATCCGGAACTCAGGAGTTACGCTCAGAGC
  CAAGGTTGGTGGACGGGAGAGGGCGAGTTCAATTTTTCCGAAGTCTTTTCTCCAGTTGAGGATCATCTAG
  ACTGCGGTGCTGGCAAAGACAGCTTAGAAAAACAAGAAGAAAGCATCACAGTGCAGACTATGATGAACAC
  CTTACGGGACAAAGCCAGCGGAGTGTGCATAGACTCTGAGTTTTTCCTCACCACAGCCAGTGGAGTGTCT
  GTCCTGCCGCAGAATAGAAGCTCTCCGTGCATTCACTACTTCACTGGAACCCATGATCCTTCCAGGTCCA
  TATTCAAGCCTTTCATCTTTGTTGATGACGTAAAACTTGTCCCCAAAACACAGTCTCCCTGTTTTGGGGA
  TGACGACCCTGCCAAAAAGGAGCCTCGGTTCCAGGAGAAACCAGACCGCCGGCATGAGCTGTACAAAGCC
  CACGAGTGGGCACGTGCCATCATCGAAAGTGACCAGGAGCAAGGTCGCAAGCTGAGGAGCACCATGCTGG
  AGCTGGAGAAGCAAGGCCTGGAAGCCATGGAAGAAATCCTGACCAGCTCCGAGCCACTGGACCCTGCGGA
  AGTGGGGGACCTTTTCTATGACTGTGTTGACACGGAGATTAAGTTCTTTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206268 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAPPSYCFVAFPPRAKDGLVVFGKNSARPRDEVQEVVYFSAADHEPESKVECTYISIDQVPRTYAIMI
  SRPAWLWGAEMGANEHGVCIANEAINTREPAAEIEALLGMDLVRLGLERGETAKEALDVIVSLLEEHGQG
  GNYFEDANSCHSFQSAYLIVDRDEAWVLETIGKYWAAEKVTEGVRCICSQLSLTTKMDAEHPELRSYAQS
  QGWWTGEGEFNFSEVFSPVEDHLDCGAGKDSLEKQEESITVQTMMNTLRDKASGVCIDSEFFLTTASGVS
  VLPQNRSSPCIHYFTGTHDPSRSIFKPFIFVDDVKLVPKTQSPCFGDDDPAKKEPRFQEKPDRRHELYKA
  HEWARAIIESDQEQGRKLRSTMLELEKQGLEAMEEILTSSEPLDPAEVGDLFYDCVDTEIKFFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014766
• ORF Size: 1242 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014766.2, NM_014766.3, NM_014766.4, NP_055581.2
• RefSeq Size: 5278 bp
• RefSeq ORF: 1245 bp
• Locus ID: 9805
• MW: 46.4 kDa
• Gene Summary: This gene likely encodes a member of the secernin family of proteins. A similar protein in rat functions in regulation of exocytosis in mast cells. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]