p35 (CDK5R1) (NM_003885) Human Tagged ORF Clone
CAT#: RC206302
CDK5R1 (Myc-DDK-tagged)-Human cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1)
"NM_003885" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CDK5R1 |
Synonyms | CDK5P35; CDK5R; NCK5A; p23; p25; p35; p35nck5a |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC206302 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCACGGTGCTGTCCCTGTCTCCCAGCTACCGGAAGGCCACGCTGTTTGAGGATGGCGCGGCCACCG TGGGCCACTATACGGCCGTACAGAACAGCAAGAACGCCAAGGACAAGAACCTGAAGCGCCACTCCATCAT CTCCGTGCTGCCTTGGAAGAGAATCGTGGCCGTGTCGGCCAAGAAGAAGAACTCAAAGAAGGTGCAGCCC AACAGCAGCTACCAGAACAACATCACGCACCTCAACAATGAGAACCTGAAGAAGTCGCTGTCGTGCGCCA ACCTGTCCACATTCGCCCAGCCCCCACCGGCCCAGCCGCCTGCACCCCCGGCCAGCCAGCTCTCGGGTTC CCAGACCGGGGGCTCCTCCTCAGTCAAGAAAGCCCCTCACCCTGCCGTCACCTCCGCAGGGACGCCCAAA CGGGTCATCGTCCAGGCGTCCACCAGTGAGCTGCTTCGCTGCCTGGGTGAGTTTCTCTGCCGCCGGTGCT ACCGCCTGAAGCACCTGTCCCCCACGGACCCCGTGCTCTGGCTGCGCAGCGTGGACCGCTCGCTGCTTCT GCAGGGCTGGCAGGACAAGGGCTTCATCACGCCGGCCAACGTGGTCTTCCTCTACATGCTCTGCAGGGAT GTTATCTCCTCCGAGGTGGGCTCGGATCACGAGCTCCAGGCCGTCCTGCTGACATGCCTGTACCTCTCCT ACTCCTACATGGGCAACGAGATCTCCTACCCGCTCAAGCCCTTCCTGGTGGAGAGCTGCAAGGAGGCCTT TTGGGACCGTTGCCTCTCTGTCATCAACCTCATGAGCTCAAAGATGCTGCAGATAAATGCCGACCCACAC TACTTCACACAGGTCTTCTCCGACCTGAAGAACGAGAGCGGCCAGGAGGACAAGAAGCGGCTCCTCCTAG GCCTGGATCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206302 protein sequence
Red=Cloning site Green=Tags(s) MGTVLSLSPSYRKATLFEDGAATVGHYTAVQNSKNAKDKNLKRHSIISVLPWKRIVAVSAKKKNSKKVQP NSSYQNNITHLNNENLKKSLSCANLSTFAQPPPAQPPAPPASQLSGSQTGGSSSVKKAPHPAVTSAGTPK RVIVQASTSELLRCLGEFLCRRCYRLKHLSPTDPVLWLRSVDRSLLLQGWQDKGFITPANVVFLYMLCRD VISSEVGSDHELQAVLLTCLYLSYSYMGNEISYPLKPFLVESCKEAFWDRCLSVINLMSSKMLQINADPH YFTQVFSDLKNESGQEDKKRLLLGLDR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_003885 |
ORF Size | 921 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003885.1, NM_003885.2, NP_003876.1 |
RefSeq Size | 3870 bp |
RefSeq ORF | 924 bp |
Locus ID | 8851 |
Domains | CDK5_activator |
Protein Families | Druggable Genome |
Protein Pathways | Alzheimer's disease |
MW | 34.1 kDa |
Gene Summary | The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC117693 | CDK5R1 (untagged)-Human cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1) |
USD 310.00 |
|
RG206302 | CDK5R1 (GFP-tagged) - Human cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1) |
USD 460.00 |
|
RC206302L1 | Lenti ORF clone of Human cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1), Myc-DDK-tagged |
USD 768.00 |
|
RC206302L2 | Lenti ORF clone of Human cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1), mGFP tagged |
USD 768.00 |
|
RC206302L3 | Lenti ORF clone of Human cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1), Myc-DDK-tagged |
USD 620.00 |
|
RC206302L4 | Lenti ORF clone of Human cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1), mGFP tagged |
USD 620.00 |
{0} Product Review(s)
Be the first one to submit a review