CPEB1 (NM_001079535) Human Tagged ORF Clone

CAT#: RC206406

CPEB1 (Myc-DDK-tagged)-Human cytoplasmic polyadenylation element binding protein 1 (CPEB1), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY421522) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213337 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CPEB1 protein (Cat# TP306406). The protein was produced from HEK293T cells transfected with CPEB1 cDNA clone (Cat# RC206406) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPEB1
• Synonyms: CPE-BP1; CPEB; CPEB-1; h-CPEB; hCPEB-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206406 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTTCCCAACCTCTGCGCAAGAATCTTCCCGTGGCCTCCCAGATGCAAATGACTTGTGCCTTGGCC
  TGCAGTCCCTCAGTCTGACAGGCTGGGACCGACCCTGGAGCACCCAGGACTCAGATTCCTCAGCCCAGAG
  CAGCACACACTCGGTACTGAGCATGCTCCATAACCCACTGGGAAATGTCCTAGGAAAACCCCCCTTGAGC
  TTCCTGCCTCTGGATCCCCTTGGGTCTGACTTGGTGGACAAGTTTCCAGCACCCTCAGTTAGAGGATCAC
  GCCTGGACACCCGGCCCATCCTGGACTCTCGATCTAGCAGCCCCTCTGACTCAGACACCAGTGGCTTCAG
  CTCTGGATCAGATCATCTCTCAGATTTGATTTCAAGCCTTCGCATTTCTCCACCTCTGCCCTTCCTGTCT
  CTGTCAGGGGGTGGTCCCAGAGACCCTTTAAAGATGGGGGTAGGGTCTCGGATGGACCAAGAGCAAGCTG
  CTCTTGCTGCAGTCACTCCCTCCCCAACCAGTGCTTCAAAGAGATGGCCAGGAGCTTCTGTGTGGCCATC
  CTGGGACCTCCTCGAAGCTCCCAAAGACCCCTTCAGCATAGAGAGAGAGGCCAGGCTGCACCGACAAGCT
  GCAGCTGTGAATGAAGCCACCTGTACCTGGAGTGGCCAGCTTCCTCCCCGGAACTATAAGAACCCCATCT
  ACTCTTGCAAGGTGTTTCTAGGAGGTGTTCCTTGGGATATTACAGAAGCTGGATTAGTTAACACCTTCCG
  TGTTTTTGGCTCTTTGAGTGTGGAGTGGCCTGGTAAGGATGGCAAGCATCCCCGGTGTCCTCCCAAAGGG
  TATGTGTATCTGGTCTTCGAACTAGAGAAGTCTGTCCGATCCTTGCTTCAGGCTTGCTCTCATGACCCGC
  TGAGCCCAGATGGCCTGAGTGAATATTATTTCAAGATGTCCAGCCGAAGGATGCGCTGCAAGGAGGTGCA
  GGTGATCCCCTGGGTATTAGCCGACAGTAACTTTGTCCGGAGCCCATCTCAGAGGCTTGACCCCAGCAGG
  ACGGTGTTTGTCGGTGCTCTGCATGGAATGCTAAATGCTGAGGCCCTGGCAGCCATCTTGAACGACCTAT
  TTGGTGGAGTGGTGTATGCCGGGATTGACACAGATAAGCACAAGTATCCCATTGGTTCTGGTCGTGTGAC
  TTTCAATAACCAACGGAGTTACCTGAAAGCAGTCAGCGCTGCTTTTGTGGAGATCAAAACCACCAAGTTC
  ACAAAGAAGGTTCAGATTGACCCCTACCTGGAAGATTCTCTGTGTCATATCTGCAGTTCTCAGCCTGGTC
  CTTTCTTCTGTCGAGATCAGGTCTGCTTCAAATACTTCTGCCGGAGCTGCTGGCACTGGCGGCACAGCAT
  GGAGGGCCTGCGCCACCACAGCCCCCTGATGCGGAACCAGAAGAACCGAGATTCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206406 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLFPTSAQESSRGLPDANDLCLGLQSLSLTGWDRPWSTQDSDSSAQSSTHSVLSMLHNPLGNVLGKPPLS
  FLPLDPLGSDLVDKFPAPSVRGSRLDTRPILDSRSSSPSDSDTSGFSSGSDHLSDLISSLRISPPLPFLS
  LSGGGPRDPLKMGVGSRMDQEQAALAAVTPSPTSASKRWPGASVWPSWDLLEAPKDPFSIEREARLHRQA
  AAVNEATCTWSGQLPPRNYKNPIYSCKVFLGGVPWDITEAGLVNTFRVFGSLSVEWPGKDGKHPRCPPKG
  YVYLVFELEKSVRSLLQACSHDPLSPDGLSEYYFKMSSRRMRCKEVQVIPWVLADSNFVRSPSQRLDPSR
  TVFVGALHGMLNAEALAAILNDLFGGVVYAGIDTDKHKYPIGSGRVTFNNQRSYLKAVSAAFVEIKTTKF
  TKKVQIDPYLEDSLCHICSSQPGPFFCRDQVCFKYFCRSCWHWRHSMEGLRHHSPLMRNQKNRDSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079535
• ORF Size: 1458 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079535.1, NP_001073003.1
• RefSeq Size: 3196 bp
• RefSeq ORF: 1461 bp
• Locus ID: 64506
• Protein Pathways: Dorso-ventral axis formation, Oocyte meiosis, Progesterone-mediated oocyte maturation
• MW: 53.6 kDa
• Gene Summary: This gene encodes a member of the cytoplasmic polyadenylation element binding protein family. This highly conserved protein binds to a specific RNA sequence, called the cytoplasmic polyadenylation element, found in the 3' untranslated region of some mRNAs. The encoded protein functions in both the cytoplasm and the nucleus. It is involved in the regulation of mRNA translation, as well as processing of the 3' untranslated region, and may play a role in cell proliferation and tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]