CHST15 (NM_015892) Human Tagged ORF Clone

CAT#: RC206489

CHST15 (Myc-DDK-tagged)-Human carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 (CHST15), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY414286) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206489 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CHST15 protein (Cat# TP306489). The protein was produced from HEK293T cells transfected with CHST15 cDNA clone (Cat# RC206489) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CHST15
• Synonyms: BRAG; GALNAC4S-6ST
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206489 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCACTGCATTAATTGCTGCATACAGCTGTTACCCGACGGCGCACACAAGCAGCAGGTCAACTGCC
  AAGGGGGCCCCCATCACGGTCACCAGGCGTGCCCCACGTGCAAAGGAGAAAACAAAATTCTGTTTCGTGT
  GGACAGTAAGCAGATGAACTTGCTTGCTGTTCTCGAAGTGAGGACTGAAGGGAACGAAAACTGGGGTGGG
  TTTTTGCGCTTCAAAAAGGGGAAGCGATGTAGCCTCGTTTTTGGACTGATAATAATGACCTTGGTAATGG
  CTTCTTACATCCTTTCTGGGGCCCACCAAGAGCTTCTGATCTCATCACCTTTCCATTACGGAGGCTTCCC
  CAGCAACCCCAGCTTGATGGACAGCGAAAACCCAAGTGACACAAAGGAGCATCACCACCAATCCTCTGTA
  AATAATATTTCATACATGAAGGACTATCCAAGCATTAAATTAATTATCAACAGCATCACAACTAGGATTG
  AGTTCACGACCAGACAGCTCCCAGACTTAGAAGACCTTAAGAAGCAGGAGTTGCATATGTTTTCAGTCAT
  CCCCAACAAATTCCTTCCAAACAGTAAGAGCCCCTGTTGGTACGAGGAGTTCTCGGGGCAGAACACCACC
  GACCCCTACCTCACCAACTCCTACGTGCTCTACTCCAAGCGCTTCCGCTCCACCTTCGACGCCCTGCGCA
  AGGCCTTCTGGGGCCACCTGGCGCACGCGCACGGGAAGCACTTCCGCCTGCGCTGCCTGCCGCACTTCTA
  CATCATAGGGCAGCCCAAGTGCGGGACCACAGACCTCTATGACCGCCTGCGGCTGCACCCTGAGGTCAAG
  TTCTCCGCCATCAAGGAGCCACACTGGTGGACCCGGAAGCGCTTTGGAATCGTCCGCCTAAGAGATGGGC
  TGCGAGACCGCTATCCCGTGGAAGATTATCTGGACCTCTTTGACCTGGCCGCACACCAGATCCATCAAGG
  ACTGCAGGCCAGCTCTGCAAAGGAGCAGAGCAAGATGAATACAATCATTATCGGGGAGGCCAGTGCCTCC
  ACGATGTGGGATAATAATGCCTGGACGTTCTTCTACGACAACAGCACGGATGGCGAGCCACCGTTTCTGA
  CGCAGGACTTCATCCACGCCTTTCAGCCAAATGCCAGACTGATTGTCATGCTCAGGGACCCTGTGGAGAG
  GTTGTACTCAGACTATCTCTACTTTGCAAGTTCGAATAAATCCGCGGACGACTTCCATGAGAAAGTGACA
  GAAGCACTGCAGCTGTTTGAAAATTGCATGCTTGATTATTCACTGCGCGCCTGCGTCTACAACAACACCC
  TCAACAACGCCATGCCTGTGAGGCTCCAGGTTGGGCTCTATGCTGTGTACCTTCTGGACTGGCTCAGCGT
  TTTTGACAAGCAACAGTTTCTCATTCTTCGCCTGGAAGATCATGCATCCAACGTCAAGTACACCATGCAC
  AAGGTCTTCCAGTTTCTGAACCTAGGGCCCTTAAGTGAGAAGCAGGAGGCTTTGATGACCAAGAGCCCCG
  CATCCAATGCACGGCGTCCCGAGGACCGGAACCTGGGGCCCATGTGGCCCATCACACAGAAGATTCTGCG
  GGATTTCTACAGGCCCTTCAACGCTAGGCTGGCGCAGGTCCTCGCGGATGAGGCGTTTGCGTGGAAGACG
  ACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206489 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRHCINCCIQLLPDGAHKQQVNCQGGPHHGHQACPTCKGENKILFRVDSKQMNLLAVLEVRTEGNENWGG
  FLRFKKGKRCSLVFGLIIMTLVMASYILSGAHQELLISSPFHYGGFPSNPSLMDSENPSDTKEHHHQSSV
  NNISYMKDYPSIKLIINSITTRIEFTTRQLPDLEDLKKQELHMFSVIPNKFLPNSKSPCWYEEFSGQNTT
  DPYLTNSYVLYSKRFRSTFDALRKAFWGHLAHAHGKHFRLRCLPHFYIIGQPKCGTTDLYDRLRLHPEVK
  FSAIKEPHWWTRKRFGIVRLRDGLRDRYPVEDYLDLFDLAAHQIHQGLQASSAKEQSKMNTIIIGEASAS
  TMWDNNAWTFFYDNSTDGEPPFLTQDFIHAFQPNARLIVMLRDPVERLYSDYLYFASSNKSADDFHEKVT
  EALQLFENCMLDYSLRACVYNNTLNNAMPVRLQVGLYAVYLLDWLSVFDKQQFLILRLEDHASNVKYTMH
  KVFQFLNLGPLSEKQEALMTKSPASNARRPEDRNLGPMWPITQKILRDFYRPFNARLAQVLADEAFAWKT
  T
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015892
• ORF Size: 1683 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015892.2, NM_015892.3, NM_015892.4, NP_056976.2
• RefSeq Size: 4813 bp
• RefSeq ORF: 1686 bp
• Locus ID: 51363
• Domains: Sulfotransfer
• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis
• MW: 64.9 kDa
• Gene Summary: Chondroitin sulfate (CS) is a glycosaminoglycan which is an important structural component of the extracellular matrix and which links to proteins to form proteoglycans. Chondroitin sulfate E (CS-E) is an isomer of chondroitin sulfate in which the C-4 and C-6 hydroxyl groups are sulfated. This gene encodes a type II transmembrane glycoprotein that acts as a sulfotransferase to transfer sulfate to the C-6 hydroxal group of chondroitin sulfate. This gene has also been identified as being co-expressed with RAG1 in B-cells and as potentially acting as a B-cell surface signaling receptor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]