POMT2 (NM_013382) Human Tagged ORF Clone

CAT#: RC206498

POMT2 (Myc-DDK-tagged)-Human protein-O-mannosyltransferase 2 (POMT2)

Product Images

Western blot validation of overexpression lysate (Cat# LY402256) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206498 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POMT2
• Synonyms: LGMD2N; LGMDR14; MDDGA2; MDDGB2; MDDGC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206498 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCCGGCCACGGGCGGAGGCCTGGCAGAGTCCGAGCTGCGTCCCCGGAGGGGCCGCTGTGGCCCCC
  AGGCTGCTAGGGCCGCAGGCCGGGACGTGGCCGCTGAGGCTGTGGCGCGAAGCCCCAAACGGCCTGCTTG
  GGGCTCACGGCGCTTCGAGGCTGTCGGCTGGTGGGCCCTGCTGGCCTTGGTGACGCTGCTGTCCTTCGCC
  ACCCGCTTCCACCGCTTGGACGAGCCGCCGCACATCTGTTGGGATGAGACTCACTTTGGAAAAATGGGAA
  GTTACTATATCAACCGTACATTTTTCTTTGATGTGCACCCGCCCCTGGGAAAGATGCTGATAGGTCTTGC
  TGGCTACCTGAGTGGATATGATGGTACCTTTTTGTTCCAGAAGCCTGGGGATAAATATGAGCATCACAGC
  TACATGGGAATGAGAGGATTCTGTGCATTCCTTGGCTCCTGGCTGGTCCCCTTTGCCTACCTCACTGTAC
  TGGATCTGTCCAAGTCCCTCTCGGCAGCACTGCTCACAGCTGCCCTCCTCACCTTTGACACGGGATGCCT
  CACTCTGTCCCAGTACATCCTCCTTGACCCCATCCTGATGTTCTTCATCATGGCTGCCATGCTGAGCATG
  GTCAAGTACAACTCTTGCGCCGACAGGCCCTTCTCTGCCCCCTGGTGGTTCTGGCTCAGCCTGACTGGCG
  TTAGTCTTGCTGGTGCTTTAGGGGTCAAGTTTGTTGGCCTCTTTATCATCCTTCAAGTGGGGCTGAACAC
  CATTGCAGACCTTTGGTACCTGTTCGGAGACCTCAGTCTTTCATTGGTGACTGTGGGAAAACACCTGACT
  GCTCGTGTCCTGTGCCTCATAGTGCTGCCCCTGGCTCTCTATACAGCCACCTTTGCTGTTCACTTCATGG
  TGCTGAGTAAAAGTGGCCCTGGTGACGGTTTCTTCAGTTCTGCCTTCCAGGCCCGGCTTTCAGGGAACAA
  CCTGCACAATGCTTCCATCCCTGAACACCTGGCCTACGGCTCTGTGATCACTGTGAAGAACCTCCGGATG
  GCCATCGGCTATCTGCACTCCCACAGGCACCTCTACCCCGAGGGCATTGGTGCCCGTCAGCAGCAGGTCA
  CCACCTATTTGCACAAGGACTACAACAACCTGTGGATTATCAAGAAACATAACACAAACTCAGATCCCCT
  AGACCCTTCCTTCCCAGTGGAGTTTGTAAGACATGGAGACATTATTCGACTAGAACACAAAGAAACTTCC
  CGGAACTTGCACAGTCACTATCATGAGGCCCCCATGACCCGGAAGCACTATCAGGTCACCGGCTATGGCA
  TAAATGGAACAGGGGACTCAAATGATTTCTGGCGGATTGAGGTCGTAAACAGAAAATTTGGAAACCGGAT
  CAAAGTGCTGAGAAGTCGAATTCGCTTCATCCATTTGGTCACAGGTTGTGTCCTGGGCTCCTCGGGAAAG
  GTTCTGCCCAAGTGGGGCTGGGAGCAGTTGGAAGTTACTTGCACCCCATACCTGAAAGAAACCCTCAACT
  CCATCTGGAATGTGGAGGACCATATCAATCCCAAGTTGCCAAACATCAGCCTGGATGTGCTACAGCCCAG
  TTTTCCTGAGATCTTGCTGGAATCCCACATGGTCATGATCCGGGGGAACAGTGGCCTCAAACCCAAGGAC
  AATGAGTTCACGTCCAAACCCTGGCACTGGCCTATCAACTATCAGGGCCTACGCTTCTCAGGGGTCAATG
  ACACAGATTTCCGAGTCTATCTGCTTGGCAACCCGGTGGTTTGGTGGCTGAATCTGTTGAGCATCGCCCT
  CTACCTCCTCTCAGGGAGCATCATTGCTGTAGCCATGCAGAGAGGGGCACGGCTGCCAGCGGAGGTTGCA
  GGGTTGTCCCAGGTCCTGCTGCGAGGAGGCGGCCAGGTCCTGCTCGGCTGGACACTCCATTACTTCCCGT
  TTTTCCTGATGGGCCGGGTCCTCTACTTCCACCACTACTTCCCAGCCATGCTCTTCTCAAGCATGTTGAC
  AGGCATTCTGTGGGACACCCTCCTGCGGCTCTGTGCCTGGGGCTTGGCCTCATGGCCCCTGGCGAGGGGC
  ATACATGTGGCGGGAATCCTGAGCCTGCTCCTGGGAACTGCCTACAGCTTCTACCTCTTCCACCCTCTGG
  CTTACGGGATGGTTGGTCCCCTGGCCCAGGACCCCCAAAGTCCAATGGCAGGACTAAGGTGGCTGGACTC
  ATGGGACTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206498 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPPATGGGLAESELRPRRGRCGPQAARAAGRDVAAEAVARSPKRPAWGSRRFEAVGWWALLALVTLLSFA
  TRFHRLDEPPHICWDETHFGKMGSYYINRTFFFDVHPPLGKMLIGLAGYLSGYDGTFLFQKPGDKYEHHS
  YMGMRGFCAFLGSWLVPFAYLTVLDLSKSLSAALLTAALLTFDTGCLTLSQYILLDPILMFFIMAAMLSM
  VKYNSCADRPFSAPWWFWLSLTGVSLAGALGVKFVGLFIILQVGLNTIADLWYLFGDLSLSLVTVGKHLT
  ARVLCLIVLPLALYTATFAVHFMVLSKSGPGDGFFSSAFQARLSGNNLHNASIPEHLAYGSVITVKNLRM
  AIGYLHSHRHLYPEGIGARQQQVTTYLHKDYNNLWIIKKHNTNSDPLDPSFPVEFVRHGDIIRLEHKETS
  RNLHSHYHEAPMTRKHYQVTGYGINGTGDSNDFWRIEVVNRKFGNRIKVLRSRIRFIHLVTGCVLGSSGK
  VLPKWGWEQLEVTCTPYLKETLNSIWNVEDHINPKLPNISLDVLQPSFPEILLESHMVMIRGNSGLKPKD
  NEFTSKPWHWPINYQGLRFSGVNDTDFRVYLLGNPVVWWLNLLSIALYLLSGSIIAVAMQRGARLPAEVA
  GLSQVLLRGGGQVLLGWTLHYFPFFLMGRVLYFHHYFPAMLFSSMLTGILWDTLLRLCAWGLASWPLARG
  IHVAGILSLLLGTAYSFYLFHPLAYGMVGPLAQDPQSPMAGLRWLDSWDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013382
• ORF Size: 2250 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013382.1, NM_013382.2, NM_013382.3, NM_013382.4, NM_013382.5, NP_037514.2
• RefSeq Size: 4874 bp
• RefSeq ORF: 2253 bp
• Locus ID: 29954
• Protein Families: Transmembrane
• Protein Pathways: O-Mannosyl glycan biosynthesis
• MW: 84.2 kDa
• Gene Summary: The protein encoded by this gene is an O-mannosyltransferase that requires interaction with the product of the POMT1 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS). [provided by RefSeq, Oct 2008]