PLA2G2D (NM_012400) Human Tagged ORF Clone
CAT#: RC206500L4
- LentiORF®
Lenti ORF clone of Human phospholipase A2, group IID (PLA2G2D), mGFP tagged
"NM_012400" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | PLA2G2D |
Synonyms | PLA2IID; sPLA2-IID; sPLA2S; SPLASH |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC206500).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_012400 |
ORF Size | 435 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012400.2 |
RefSeq Size | 1985 bp |
RefSeq ORF | 438 bp |
Locus ID | 26279 |
UniProt ID | Q9UNK4 |
Cytogenetics | 1p36.12 |
Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
Protein Pathways | alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway |
MW | 16.5 kDa |
Gene Summary | This gene encodes a secreted member of the phospholipase A2 family, and is found in a cluster of related family members on chromosome 1. Phospholipase A2 family members hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce lysophospholipids and free fatty acid. This gene may be involved in inflammation and immune response, and in weight loss associated with chronic obstructive pulmonary disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC122785 | PLA2G2D (untagged)-Human phospholipase A2, group IID (PLA2G2D) |
USD 310.00 |
|
RC206500 | PLA2G2D (Myc-DDK-tagged)-Human phospholipase A2, group IID (PLA2G2D) |
USD 98.00 |
|
RG206500 | PLA2G2D (GFP-tagged) - Human phospholipase A2, group IID (PLA2G2D) |
USD 460.00 |
|
RC206500L3 | Lenti ORF clone of Human phospholipase A2, group IID (PLA2G2D), Myc-DDK-tagged |
USD 620.00 |
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