KCNE1L (KCNE5) (NM_012282) Human Tagged ORF Clone
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | KCNE5 |
Synonyms | KCNE1L |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC206502 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTGCAGCGAGAGCCAGCGGCTGCGAACCCTTCTGAGCCGCCTGTTGCTCGAGCTGCACCACCGGG GTAATGCCAGCGGCTTGGGCGCTGGCCCTCGTCCCAGCATGGGCATGGGGGTCGTGCCTGACCCTTTCGT GGGCCGCGAGGTGACCAGCGCCAAGGGCGACGACGCCTATCTCTACATCCTGCTCATCATGATCTTCTAC GCCTGCTTGGCCGGAGGCCTCATCCTGGCCTACACCCGCTCCCGTAAGCTCGTCGAGGCCAAGGACGAGC CGTCCCAGGCTTGCGCCGAGCACGAATGGGCCCCGGGAGGCGCCCTGACCGCCGACGCCGAGGCTGCCGC GGGCTCCCAGGCCGAGGGCCGCCGCCAGCTTGCCTCCGAGGGGCTGCCTGCCCTCGCCCAGGGCGCTGAG CGGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206502 protein sequence
Red=Cloning site Green=Tags(s) MNCSESQRLRTLLSRLLLELHHRGNASGLGAGPRPSMGMGVVPDPFVGREVTSAKGDDAYLYILLIMIFY ACLAGGLILAYTRSRKLVEAKDEPSQACAEHEWAPGGALTADAEAAAGSQAEGRRQLASEGLPALAQGAE RV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_012282 |
ORF Size | 426 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012282.1, NM_012282.2, NM_012282.3, NP_036414.1 |
RefSeq Size | 1465 bp |
RefSeq ORF | 429 bp |
Locus ID | 23630 |
Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
MW | 15 kDa |
Gene Summary | This gene encodes a member of a family of single pass transmembrane domain proteins that function as ancillary subunits to voltage-gated potassium channels. Members of this family affect diverse processes in potassium channel regulation, including ion selectivity, voltage dependence, and anterograde recycling from the plasma membrane. Variants of this gene are associated with idiopathic ventricular fibrillation and Brugada syndrome. [provided by RefSeq, Nov 2016] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC122784 | KCNE1L (untagged)-Human KCNE1-like (KCNE1L) |
USD 420.00 |
|
RG206502 | KCNE1L (GFP-tagged) - Human KCNE1-like (KCNE1L) |
USD 460.00 |
|
RC206502L1 | Lenti ORF clone of Human KCNE1-like (KCNE1L), Myc-DDK-tagged |
USD 620.00 |
|
RC206502L2 | Lenti ORF clone of Human KCNE1-like (KCNE1L), mGFP tagged |
USD 620.00 |
|
RC206502L3 | Lenti ORF clone of Human KCNE1-like (KCNE1L), Myc-DDK-tagged |
USD 620.00 |
|
RC206502L4 | Lenti ORF clone of Human KCNE1-like (KCNE1L), mGFP tagged |
USD 620.00 |
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