BS69 (ZMYND11) (NM_212479) Human Tagged ORF Clone

CAT#: RC206521

ZMYND11 (Myc-DDK-tagged)-Human zinc finger, MYND-type containing 11 (ZMYND11), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY403950) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206521 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZMYND11
• Synonyms: BRAM1; BS69; MRD30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206521 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGAGTCCACGGTATGCACCCTAAAGAGACCACCCGTCAGCTGAGCTTAGCTGTGAAAGATGGTC
  TTATTGTCGAAACTCTAACAGTGGGCTGCAAAGGTTCAAAAGCTGGTATTGAACAAGAAGGATATTGGTT
  GCCAGGAGATGAGATTGACTGGGAAACAGAAAATCATGACTGGTATTGTTTTGAATGCCATTTGCCTGGA
  GAGGTGTTGATATGTGACCTGTGTTTTCGTGTGTATCATTCCAAGTGTTTGTCTGATGAGTTCAGGCTTA
  GAGACAGCAGTAGTCCCTGGCAGTGCCCAGTTTGCAGGAGCATTAAGAAGAAGAATACAAACAAACAGGA
  GATGGGCACATACCTCAGATTCATTGTCTCCCGCATGAAGGAGAGGGCTATAGATCTTAATAAAAAGGGG
  AAGGACAATAAACACCCGATGTACAGGAGGCTGGTGCACTCAGCTGTGGACGTTCCCACCATTCAAGAGA
  AAGTGAATGAAGGGAAATACCGAAGTTATGAAGAGTTCAAAGCTGATGCCCAATTGCTTCTCCACAATAC
  CGTGATTTTCTATGGAGACAGTGAGCAAGCTGACATTGCGAGGATGCTATATAAAGACACATGTCATGAG
  CTGGATGAACTGCAGCTTTGCAAGAATTGCTTTTACTTGTCAAATGCTCGTCCTGACAACTGGTTCTGTT
  ATCCTTGTATACCTAATCATGAGCTGGTTTGGGCTAAAATGAAAGGTTTTGGGTTTTGGCCAGCCAAAGT
  CATGCAGAAAGAAGACAATCAAGTCGACGTTCGCTTCTTTGGCCACCACCACCAGAGGGCCTGGATTCCT
  TCTGAAAACATTCAAGATATCACAGTCAACATTCATCGGCTGCACGTGAAGCGCAGTATGGGTTGGAAAA
  AGGCCTGTGATGAGCTGGAGCTGCATCAGCGTTTCCTACGAGAAGGGAGATTTTGGAAATCTAAGAATGA
  GGACCGAGGTGAGGAAGAGGCAGAATCCAGTATCTCCTCCACCAGTAATGAGCAGCTAAAGGTCACTCAA
  GAACCAAGAGCAAAGAAAGGACGACGTAATCAAAGTGTGGAGCCCAAAAAGGAAGAACCAGAGCCTGAAA
  CAGAAGCAGTAAGTTCTAGCCAGGAAATACCCACGATGCCTCAGCCCATCGAAAAAGTCTCCGTGTCAAC
  TCAGACAAAGAAGTTAAGTGCCTCTTCACCAAGAATGCTGCATCGGAGCACCCAGACCACAAACGACGGC
  GTGTGTCAGAGCATGTGCCATGACAAATACACCAAGATCTTCAATGACTTCAAAGACCGGATGAAGTCGG
  ACCACAAGCGGGAGACAGAGCGTGTTGTCCGAGAAGCTCTGGAGAAGCTGCGTTCTGAAATGGAAGAAGA
  AAAGAGACAAGCTGTAAATAAAGCTGTAGCCAACATGCAGGGTGAGATGGACAGAAAATGTAAGCAAGTA
  AAGGAAAAGTGTAAGGAAGAATTTGTAGAAGAAATCAAGAAGCTGGCAACACAGCACAAGCAACTGATTT
  CTCAGACCAAGAAGAAGCAGTGGGTAAATACCAGTCTTTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206521 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRVHGMHPKETTRQLSLAVKDGLIVETLTVGCKGSKAGIEQEGYWLPGDEIDWETENHDWYCFECHLPG
  EVLICDLCFRVYHSKCLSDEFRLRDSSSPWQCPVCRSIKKKNTNKQEMGTYLRFIVSRMKERAIDLNKKG
  KDNKHPMYRRLVHSAVDVPTIQEKVNEGKYRSYEEFKADAQLLLHNTVIFYGDSEQADIARMLYKDTCHE
  LDELQLCKNCFYLSNARPDNWFCYPCIPNHELVWAKMKGFGFWPAKVMQKEDNQVDVRFFGHHHQRAWIP
  SENIQDITVNIHRLHVKRSMGWKKACDELELHQRFLREGRFWKSKNEDRGEEEAESSISSTSNEQLKVTQ
  EPRAKKGRRNQSVEPKKEEPEPETEAVSSSQEIPTMPQPIEKVSVSTQTKKLSASSPRMLHRSTQTTNDG
  VCQSMCHDKYTKIFNDFKDRMKSDHKRETERVVREALEKLRSEMEEEKRQAVNKAVANMQGEMDRKCKQV
  KEKCKEEFVEEIKKLATQHKQLISQTKKKQWVNTSLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_212479
• ORF Size: 675 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_212479.1, NP_997644.1
• RefSeq Size: 2813 bp
• RefSeq ORF: 1704 bp
• Locus ID: 10771
• Protein Families: Druggable Genome, Transcription Factors
• MW: 61.8 kDa
• Gene Summary: The protein encoded by this gene was first identified by its ability to bind the adenovirus E1A protein. The protein localizes to the nucleus. It functions as a transcriptional repressor, and expression of E1A inhibits this repression. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]