HABP2 (NM_004132) Human Tagged ORF Clone

CAT#: RC206545

HABP2 (Myc-DDK-tagged)-Human hyaluronan binding protein 2 (HABP2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY401333) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206545 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HABP2
• Synonyms: FSAP; HABP; HGFAL; NMTC5; PHBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206545 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGCCAGGATGTCTGATCTCCATGTTCTGCTGTTAATGGCTCTGGTGGGAAAGACAGCCTGTGGGT
  TCTCCCTGATGTCTTTATTGGAAAGCCTGGACCCAGACTGGACCCCTGACCAGTATGATTACAGCTACGA
  GGATTATAATCAGGAAGAGAACACCAGTAGCACACTTACCCACGCTGAGAATCCTGACTGGTACTACACT
  GAGGACCAAGCTGATCCATGCCAGCCCAACCCCTGTGAACACGGTGGGGACTGCCTCGTCCATGGGAGCA
  CCTTCACATGCAGCTGCCTGGCTCCTTTCTCTGGGAATAAGTGTCAGAAAGTGCAAAATACGTGCAAGGA
  CAACCCATGTGGCCGGGGCCAATGTCTCATTACCCAGAGTCCTCCCTACTACCGCTGTGTCTGTAAACAC
  CCTTACACAGGTCCCAGCTGCTCCCAAGTGGTTCCTGTATGCAGGCCAAACCCCTGCCAGAATGGGGCTA
  CCTGCTCCCGGCATAAGCGGAGATCCAAGTTCACCTGTGCCTGTCCCGACCAGTTCAAGGGGAAATTCTG
  TGAAATAGGTTCTGATGACTGCTATGTTGGCGATGGCTACTCTTACCGAGGGAAAATGAATAGGACAGTC
  AACCAGCATGCGTGCCTTTACTGGAACTCCCACCTCCTCTTGCAGGAGAATTACAACATGTTTATGGAGG
  ATGCTGAAACCCATGGGATTGGGGAACACAATTTCTGCAGAAACCCAGATGCGGACGAAAAGCCCTGGTG
  CTTTATTAAAGTTACCAATGACAAGGTGAAATGGGAATACTGTGATGTCTCAGCCTGCTCAGCCCAGGAC
  GTTGCCTACCCAGAGGAAAGCCCCACTGAGCCATCAACCAAGCTTCCGGGGTTTGACTCCTGTGGAAAGA
  CTGAGATAGCAGAGAGGAAGATCAAGAGAATCTATGGAGGCTTTAAGAGCACGGCGGGCAAGCACCCATG
  GCAGGCGTCCCTCCAGTCCTCGCTGCCTCTGACCATCTCCATGCCCCAGGGCCACTTCTGTGGTGGGGCG
  CTGATCCACCCCTGCTGGGTGCTCACTGCTGCCCACTGCACCGACATAAAAACCAGACATCTAAAGGTGG
  TGCTAGGGGACCAGGACCTGAAGAAAGAAGAATTTCATGAGCAGAGCTTTAGGGTGGAGAAGATATTCAA
  GTACAGCCACTACAATGAAAGAGATGAGATTCCCCACAATGATATTGCATTGCTCAAGTTAAAGCCAGTG
  GATGGTCACTGTGCTCTAGAATCCAAATACGTGAAGACTGTGTGCTTGCCTGATGGGTCCTTTCCCTCTG
  GGAGTGAGTGCCACATCTCTGGCTGGGGTGTTACAGAAACAGGAAAAGGGTCCCGCCAGCTCCTGGATGC
  CAAAGTCAAGCTGATTGCCAACACTTTGTGCAACTCCCGCCAACTCTATGACCACATGATTGATGACAGT
  ATGATCTGTGCAGGAAATCTTCAGAAACCTGGGCAAGACACCTGCCAGGGTGACTCTGGAGGCCCCCTGA
  CCTGTGAGAAGGACGGCACCTACTACGTCTATGGGATAGTGAGCTGGGGCCTGGAGTGTGGGAAGAGGCC
  AGGGGTCTACACCCAAGTTACCAAATTCCTGAATTGGATCAAAGCCACCATCAAAAGTGAAAGTGGCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206545 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFARMSDLHVLLLMALVGKTACGFSLMSLLESLDPDWTPDQYDYSYEDYNQEENTSSTLTHAENPDWYYT
  EDQADPCQPNPCEHGGDCLVHGSTFTCSCLAPFSGNKCQKVQNTCKDNPCGRGQCLITQSPPYYRCVCKH
  PYTGPSCSQVVPVCRPNPCQNGATCSRHKRRSKFTCACPDQFKGKFCEIGSDDCYVGDGYSYRGKMNRTV
  NQHACLYWNSHLLLQENYNMFMEDAETHGIGEHNFCRNPDADEKPWCFIKVTNDKVKWEYCDVSACSAQD
  VAYPEESPTEPSTKLPGFDSCGKTEIAERKIKRIYGGFKSTAGKHPWQASLQSSLPLTISMPQGHFCGGA
  LIHPCWVLTAAHCTDIKTRHLKVVLGDQDLKKEEFHEQSFRVEKIFKYSHYNERDEIPHNDIALLKLKPV
  DGHCALESKYVKTVCLPDGSFPSGSECHISGWGVTETGKGSRQLLDAKVKLIANTLCNSRQLYDHMIDDS
  MICAGNLQKPGQDTCQGDSGGPLTCEKDGTYYVYGIVSWGLECGKRPGVYTQVTKFLNWIKATIKSESGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004132
• ORF Size: 1680 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004132.1, NM_004132.2, NM_004132.3, NM_004132.4, NP_004123.1
• RefSeq Size: 3019 bp
• RefSeq ORF: 1683 bp
• Locus ID: 3026
• Cytogenetics: 10q25.3
• Domains: KR, Tryp_SPc, EGF, EGF
• Protein Families: Druggable Genome, Protease, Secreted Protein
• MW: 62.7 kDa
• Gene Summary: 'This gene encodes a member of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by hepatocytes and proteolytically processed to generate heavy and light chains that form the mature heterodimer. Further autoproteolysis leads to smaller, inactive peptides. This extracellular protease binds hyaluronic acid and may play a role in the coagulation and fibrinolysis systems. Mutations in this gene are associated with nonmedullary thyroid cancer and susceptibility to venous thromboembolism. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]'