ATP4B (NM_000705) Human Tagged ORF Clone

CAT#: RC206563

ATP4B (Myc-DDK-tagged)-Human ATPase, H+/K+ exchanging, beta polypeptide (ATP4B)

Product Images

Western blot validation of overexpression lysate (Cat# LY400236) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206563 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATP4B
• Synonyms: ATP6B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206563 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTCTGCAGGAGAAGAAGACGTGTGGCCAGCGCATGGAGGAGTTCCAGCGTTACTGCTGGAACC
  CGGACACGGGGCAGATGCTGGGCCGCACCCTGTCCCGGTGGGTGTGGATCAGCCTGTACTACGTGGCCTT
  CTACGTGGTGATGACTGGGCTCTTCGCCCTGTGCCTCTATGTGCTGATGCAGACAGTGGACCCGTACACA
  CCGGACTACCAAGACCAGCTACGGTCACCAGGGGTAACCTTAAGGCCGGATGTTTACGGGGAGAAAGGCC
  TGGAAATTGTCTACAACGTCTCTGATAACAGAACCTGGGCAGACCTCACACAGACTCTCCACGCCTTCCT
  AGCAGGCTACTCTCCAGCAGCCCAGGAGGACAGCATCAACTGCACCTCCGAGCAGTACTTCTTCCAGGAG
  AGTTTCCGCGCTCCCAACCACACCAAGTTCTCCTGCAAGTTCACGGCAGATATGCTGCAGAACTGCTCAG
  GCCTGGCGGATCCCAACTTCGGCTTTGAAGAAGGAAAGCCATGTTTTATTATTAAAATGAACAGGATCGT
  CAAGTTCCTCCCCAGCAACGGCTCGGCCCCCAGAGTGGACTGCGCCTTCCTGGACCAGCCCCGCGAGCTC
  GGCCAGCCGCTGCAGGTCAAGTACTACCCTCCCAACGGCACCTTCAGTCTGCACTACTTCCCTTATTACG
  GGAAGAAAGCCCAGCCCCACTACAGCAACCCCCTGGTGGCAGCGAAGCTCCTCAACATCCCCAGGAACGC
  TGAGGTCGCCATCGTGTGCAAGGTCATGGCAGAGCACGTGACCTTCAACAATCCCCACGACCCGTATGAA
  GGGAAAGTGGAGTTCAAACTCAAGATTGAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206563 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAALQEKKTCGQRMEEFQRYCWNPDTGQMLGRTLSRWVWISLYYVAFYVVMTGLFALCLYVLMQTVDPYT
  PDYQDQLRSPGVTLRPDVYGEKGLEIVYNVSDNRTWADLTQTLHAFLAGYSPAAQEDSINCTSEQYFFQE
  SFRAPNHTKFSCKFTADMLQNCSGLADPNFGFEEGKPCFIIKMNRIVKFLPSNGSAPRVDCAFLDQPREL
  GQPLQVKYYPPNGTFSLHYFPYYGKKAQPHYSNPLVAAKLLNIPRNAEVAIVCKVMAEHVTFNNPHDPYE
  GKVEFKLKIEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000705
• ORF Size: 873 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000705.1, NM_000705.2, NM_000705.3, NP_000696.1
• RefSeq Size: 1509 bp
• RefSeq ORF: 876 bp
• Locus ID: 496
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Oxidative phosphorylation
• MW: 33.4 kDa
• Gene Summary: The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008]