STX11 (NM_003764) Human Tagged ORF Clone

CAT#: RC206571

STX11 (Myc-DDK-tagged)-Human syntaxin 11 (STX11)

Product Images

Western blot validation of overexpression lysate (Cat# LY401241) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206571 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STX11
• Synonyms: FHL4; HLH4; HPLH4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206571 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGACCGGCTAGCAGAACTTCTGGACTTGTCCAAGCAATATGACCAGCAGTTCCCAGACGGGGACG
  ATGAGTTTGACTCGCCCCACGAGGACATCGTGTTCGAGACGGACCACATCCTGGAGTCCCTGTACCGAGA
  CATCCGGGACATTCAGGATGAAAACCAGCTGCTGGTGGCCGACGTGAAGCGGCTGGGAAAGCAGAACGCC
  CGCTTCCTCACGTCCATGCGGCGCCTCAGCAGCATCAAGCGCGACACCAACTCCATCGCCAAGGCCATCA
  AGGCCCGGGGCGAGGTCATCCACTGCAAGCTGCGCGCCATGAAGGAGCTGAGCGAGGCGGCTGAGGCCCA
  GCACGGCCCGCACTCGGCAGTGGCGCGCATTTCGCGGGCGCAGTACAACGCGCTCACCCTCACCTTCCAG
  CGCGCCATGCACGACTACAACCAGGCCGAGATGAAGCAGCGCGACAACTGCAAGATCCGCATCCAGCGCC
  AGCTGGAGATCATGGGCAAGGAAGTCTCGGGCGACCAGATCGAGGACATGTTCGAGCAGGGTAAGTGGGA
  CGTGTTTTCCGAGAACTTGCTGGCCGACGTGAAGGGCGCGCGGGCCGCCCTCAACGAGATCGAGAGCCGC
  CACCGCGAACTGCTGCGCCTGGAGAGCCGCATCCGCGACGTACACGAGCTCTTCTTGCAGATGGCGGTGC
  TGGTGGAGAAGCAGGCCGACACCCTGAACGTCATCGAGCTCAACGTACAAAAGACGGTCGACTACACCGG
  CCAGGCCAAGGCGCAGGTGCGGAAGGCCGTGCAGTACGAGGAGAAGAACCCCTGCCGGACCCTCTGCTGC
  TTCTGCTGTCCCTGCCTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206571 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKDRLAELLDLSKQYDQQFPDGDDEFDSPHEDIVFETDHILESLYRDIRDIQDENQLLVADVKRLGKQNA
  RFLTSMRRLSSIKRDTNSIAKAIKARGEVIHCKLRAMKELSEAAEAQHGPHSAVARISRAQYNALTLTFQ
  RAMHDYNQAEMKQRDNCKIRIQRQLEIMGKEVSGDQIEDMFEQGKWDVFSENLLADVKGARAALNEIESR
  HRELLRLESRIRDVHELFLQMAVLVEKQADTLNVIELNVQKTVDYTGQAKAQVRKAVQYEEKNPCRTLCC
  FCCPCLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003764
• ORF Size: 861 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003764.1, NM_003764.2, NM_003764.3, NP_003755.2
• RefSeq Size: 5509 bp
• RefSeq ORF: 864 bp
• Locus ID: 8676
• Domains: SynN
• Protein Families: Druggable Genome
• Protein Pathways: SNARE interactions in vesicular transport
• MW: 33.2 kDa
• Gene Summary: This gene encodes a member of the syntaxin family. Syntaxins have been implicated in the targeting and fusion of intracellular transport vesicles. This family member may regulate protein transport among late endosomes and the trans-Golgi network. Mutations in this gene have been associated with familial hemophagocytic lymphohistiocytosis. [provided by RefSeq, Jul 2008]