SIGLEC5 (NM_003830) Human Tagged ORF Clone

CAT#: RC206610

SIGLEC5 (Myc-DDK-tagged)-Human sialic acid binding Ig-like lectin 5 (SIGLEC5)

Product Images

Western blot validation of overexpression lysate (Cat# LY418402) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206610 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SIGLEC5 protein (Cat# TP306610). The protein was produced from HEK293T cells transfected with SIGLEC5 cDNA clone (Cat# RC206610) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIGLEC5
• Synonyms: CD33L2; CD170; OB-BP2; OBBP2; SIGLEC-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206610 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCCTGCTGCTGCTGCCCCTGCTGTGGGGGGGGTCCCTGCAGGAGAAGCCAGTGTACGAGCTGC
  AAGTGCAGAAGTCGGTGACGGTGCAGGAGGGCCTGTGCGTCCTTGTGCCCTGCTCCTTCTCTTACCCCTG
  GAGATCCTGGTATTCCTCTCCCCCACTCTACGTCTACTGGTTCCGGGACGGGGAGATCCCATACTACGCT
  GAGGTTGTGGCCACAAACAACCCAGACAGAAGAGTGAAGCCAGAGACCCAGGGCCGATTCCGCCTCCTTG
  GGGATGTCCAGAAGAAGAACTGCTCCCTGAGCATCGGAGATGCCAGAATGGAGGACACGGGAAGCTATTT
  CTTCCGCGTGGAGAGAGGAAGGGATGTAAAATATAGCTACCAACAGAATAAGCTGAACTTGGAGGTGACA
  GCCCTGATAGAGAAACCCGACATCCACTTTCTGGAGCCTCTGGAGTCCGGCCGCCCCACAAGGCTGAGCT
  GCAGCCTTCCAGGATCCTGTGAAGCGGGACCACCTCTCACATTCTCCTGGACGGGGAATGCCCTCAGCCC
  CCTGGACCCCGAGACCACCCGCTCCTCGGAGCTCACCCTCACCCCCAGGCCCGAGGACCATGGCACCAAC
  CTCACCTGTCAGATGAAACGCCAAGGAGCTCAGGTGACCACGGAGAGAACTGTCCAGCTCAATGTCTCCT
  ATGCACCACAGACCATCACCATCTTCAGGAACGGCATAGCCCTAGAGATCCTGCAAAACACCTCATACCT
  TCCGGTCCTGGAGGGCCAGGCTCTGCGGCTGCTCTGTGATGCTCCCAGCAACCCCCCTGCACACCTGAGC
  TGGTTCCAGGGCTCCCCTGCCCTGAACGCCACCCCCATCTCCAATACCGGGATCTTGGAGCTTCGTCGAG
  TAAGGTCTGCAGAAGAAGGAGGCTTCACCTGCCGCGCTCAGCACCCGCTGGGCTTCCTGCAAATTTTTCT
  GAATCTCTCAGTTTACTCCCTCCCACAGTTGCTGGGCCCCTCCTGCTCCTGGGAGGCTGAGGGTCTGCAC
  TGCAGATGCTCCTTTCGAGCCCGGCCGGCCCCCTCCCTGTGCTGGCGGCTTGAGGAGAAGCCGCTGGAGG
  GGAACAGCAGCCAGGGCTCATTCAAGGTCAACTCCAGCTCAGCTGGGCCCTGGGCCAACAGCTCCCTGAT
  CCTCCACGGGGGGCTCAGCTCCGACCTCAAAGTCAGCTGCAAGGCCTGGAACATCTATGGGTCCCAGAGC
  GGCTCTGTCCTGCTGCTGCAAGGGAGATCGAACCTTGGGACAGGAGTGGTTCCTGCAGCCCTTGGTGGTG
  CTGGTGTCATGGCCCTGCTCTGTATCTGTCTGTGCCTCATCTTCTTTTTAATAGTGAAAGCCCGCAGGAA
  GCAAGCAGCTGGGAGACCAGAGAAAATGGATGATGAAGACCCCATTATGGGTACCATCACCTCGGGTTCC
  AGGAAGAAGCCCTGGCCAGACAGCGCCGGAGATCAAGCATCTCCTCCTGGGGATGCCCCTCCCTTGGAAG
  AACAAAAGGAGCTCCATTATGCCTCCCTTAGTTTTTCTGAGATGAAGTCGAGGGAGCCTAAGGACCAGGA
  GGCCCCAAGCACCACGGAGTACTCGGAGATCAAGACAAGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206610 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLPLLLLPLLWGGSLQEKPVYELQVQKSVTVQEGLCVLVPCSFSYPWRSWYSSPPLYVYWFRDGEIPYYA
  EVVATNNPDRRVKPETQGRFRLLGDVQKKNCSLSIGDARMEDTGSYFFRVERGRDVKYSYQQNKLNLEVT
  ALIEKPDIHFLEPLESGRPTRLSCSLPGSCEAGPPLTFSWTGNALSPLDPETTRSSELTLTPRPEDHGTN
  LTCQMKRQGAQVTTERTVQLNVSYAPQTITIFRNGIALEILQNTSYLPVLEGQALRLLCDAPSNPPAHLS
  WFQGSPALNATPISNTGILELRRVRSAEEGGFTCRAQHPLGFLQIFLNLSVYSLPQLLGPSCSWEAEGLH
  CRCSFRARPAPSLCWRLEEKPLEGNSSQGSFKVNSSSAGPWANSSLILHGGLSSDLKVSCKAWNIYGSQS
  GSVLLLQGRSNLGTGVVPAALGGAGVMALLCICLCLIFFLIVKARRKQAAGRPEKMDDEDPIMGTITSGS
  RKKPWPDSAGDQASPPGDAPPLEEQKELHYASLSFSEMKSREPKDQEAPSTTEYSEIKTSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003830
• ORF Size: 1653 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003830.1, NM_003830.2, NM_003830.3, NP_003821.1
• RefSeq Size: 2523 bp
• RefSeq ORF: 1656 bp
• Locus ID: 8778
• Domains: ig
• Protein Families: Druggable Genome, Transmembrane
• MW: 60.7 kDa
• Gene Summary: This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012]