RTL6 (NM_032287) Human Tagged ORF Clone

CAT#: RC206668

LDOC1L (Myc-DDK-tagged)-Human leucine zipper, down-regulated in cancer 1-like (LDOC1L)

Product Images

Western blot validation of overexpression lysate (Cat# LY410230) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206668 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RTL6 protein (Cat# TP306668). The protein was produced from HEK293T cells transfected with RTL6 cDNA clone (Cat# RC206668) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RTL6
• Synonyms: dJ1033E15.2; LDOC1L; Mar6; Mart6; SIRH3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206668 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGCCGCAGACGTCCAAAGCTGAAAGCCCAGCCTTGGCAGCGTCTCCGAATGCCCAGATGGATG
  ACGTTATTGACACCCTGACCTCCCTGCGCCTCACCAACTCGGCGCTGAGGCGGGAGGCTTCCACCCTGCG
  GGCGGAGAAGGCCAATCTCACCAACATGCTGGAGAGCGTGATGGCAGAGCTGACCTTGTTACGCACCAGG
  GCGCGGATCCCGGGGGCTCTGCAGATCACCCCGCCCATCTCCTCAATTACTTCAAACGGGACTCGACCCA
  TGACCACACCTCCAACCTCTCTGCCCGAGCCCTTTTCCGGGGACCCAGGCCGGTTGGCGGGGTTCCTGAT
  GCAGATGGACAGATTCATGATCTTCCAGGCCTCCCGCTTCCCGGGTGAGGCCGAGCGTGTGGCCTTCCTT
  GTGTCTCGACTGACTGGGGAGGCGGAGAAGTGGGCTATCCCCCACATGCAACCTGACAGCCCCTTGCGCA
  ACAACTATCAGGGGTTCCTGGCAGAGTTGCGGAGAACCTACAAGTCTCCGCTCCGGCATGCGCGGCGCGC
  CCAAATCAGGAAGACTTCTGCCTCTAATAGGGCTGTGCGAGAGAGGCAGATGCTCTGCCGCCAGCTGGCC
  TCTGCGGGCACGGGGCCTTGCCCAGTGCATCCAGCTTCCAACGGGACTAGTCCAGCGCCAGCCCTGCCTG
  CCCGAGCACGGAATCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206668 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVQPQTSKAESPALAASPNAQMDDVIDTLTSLRLTNSALRREASTLRAEKANLTNMLESVMAELTLLRTR
  ARIPGALQITPPISSITSNGTRPMTTPPTSLPEPFSGDPGRLAGFLMQMDRFMIFQASRFPGEAERVAFL
  VSRLTGEAEKWAIPHMQPDSPLRNNYQGFLAELRRTYKSPLRHARRAQIRKTSASNRAVRERQMLCRQLA
  SAGTGPCPVHPASNGTSPAPALPARARNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032287
• ORF Size: 717 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032287.2, NP_115663.2
• RefSeq Size: 5355 bp
• RefSeq ORF: 720 bp
• Locus ID: 84247
• MW: 26.2 kDa