PDILT (NM_174924) Human Tagged ORF Clone

CAT#: RC206717

PDILT (Myc-DDK-tagged)-Human protein disulfide isomerase-like, testis expressed (PDILT)

Product Images

Western blot validation of overexpression lysate (Cat# LY406406) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206717 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PDILT protein (Cat# TP306717). The protein was produced from HEK293T cells transfected with PDILT cDNA clone (Cat# RC206717) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDILT
• Synonyms: PDIA7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206717 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTACTCTGGATGCCCCTGCTGCTGGTGGCCGCTTGTGTCTCTGCTGTCCACAGCTCACCAGAGG
  TTAACGCCGGTGTTTCCAGCATCCACATAACCAAGCCTGTGCACATCCTGGAGGAACGCAGTCTCCTAGT
  GCTAACGCCCGCTGGCCTGACCCAGATGCTGAACCAGACCCGCTTCCTCATGGTGCTTTTCCACAACCCA
  TCCTCAAAGCAATCCAGGAACTTGGCGGAAGAGCTGGGCAAAGCTGTGGAGATCATGGGCAAAGGCAAGA
  ATGGGATCGGCTTTGGCAAAGTGGACATTACCATAGAGAAGGAGCTTCAGCAGGAGTTTGGGATTACCAA
  GGCCCCGGAGTTGAAGCTGTTTTTTGAGGGCAACAGGTCAGAGCCCATCAGCTGCAAAGGAGTGGTTGAA
  TCTGCTGCCTTAGTCGTTTGGTTGAGACGACAAATTAGCCAGAAAGCATTTTTGTTCAACAGCAGCGAGC
  AGGTGGCAGAGTTTGTGATATCCAGGCCCTTGGTCATCGTTGGCTTCTTCCAGGATTTAGAGGAAGAAGT
  AGCAGAGTTGTTCTATGATGTGATCAAAGACTTTCCAGAGCTAACGTTTGGAGTCATAACGATTGGCAAT
  GTCATTGGGCGTTTCCACGTCACCCTTGACAGCGTCCTGGTGTTCAAAAAGGGAAAAATTGTGAACCGCC
  AAAAGCTTATTAATGACAGTACCAACAAACAGGAACTCAATCGTGTCATAAAACAGCACCTTACAGATTT
  TGTGATCGAATACAACACTGAGAATAAGGATCTGATTTCCGAGTTGCACATCATGAGTCACATGCTGCTG
  TTTGTCTCCAAAAGCTCCGAGTCATATGGTATCATAATTCAGCATTATAAGCTGGCATCAAAGGAATTCC
  AAAACAAGATCCTTTTCATCCTTGTGGATGCAGACGAACCCAGAAATGGACGTGTCTTCAAGTACTTCCG
  GGTCACAGAGGTCGATATCCCATCCGTCCAAATCCTAAACTTGAGCTCTGACGCCAGGTACAAAATGCCT
  TCAGATGACATAACCTACGAAAGCCTCAAGAAATTTGGCCGCAGCTTCCTGAGTAAAAATGCCACAAAAC
  ATCAATCCAGTGAAGAGATTCCAAAATACTGGGACCAGGGACTGGTTAAGCAGCTCGTGGGGAAGAACTT
  CAACGTAGTCGTCTTTGACAAAGAAAAGGACGTATTTGTGATGTTCTATGCACCCTGGTCTAAAAAGTGC
  AAGATGCTGTTCCCACTGTTGGAGGAATTGGGCAGAAAATATCAAAACCACTCCACAATTATCATTGCCA
  AGATCGATGTCACAGCAAATGACATTCAGCTGATGTACCTGGACCGGTACCCATTCTTCAGGCTGTTCCC
  CAGCGGCTCTCAACAAGCTGTCCTGTATAAGGGAGAACACACCCTGAAGGGCTTCTCTGACTTCCTGGAA
  AGCCACATCAAAACTAAGATTGAGGATGAGGATGAGCTGTTGTCTGTTGAGCAAAATGAAGTGATAGAAG
  AGGAAGTGCTAGCTGAGGAAAAGGAGGTGCCTATGATGAGGAAAGGGTTACCTGAACAGCAGTCGCCTGA
  GCTGGAGAACATGACCAAGTACGTATCCAAGCTGGAAGAGCCCGCTGGGAAGAAGAAAACATCTGAGGAG
  GTGGTGGTGGTGGTGGCTAAGCCAAAGGGACCTCCAGTGCAAAAGAAGAAACCAAAAGTCAAGGAAGAAC
  TT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206717 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLLWMPLLLVAACVSAVHSSPEVNAGVSSIHITKPVHILEERSLLVLTPAGLTQMLNQTRFLMVLFHNP
  SSKQSRNLAEELGKAVEIMGKGKNGIGFGKVDITIEKELQQEFGITKAPELKLFFEGNRSEPISCKGVVE
  SAALVVWLRRQISQKAFLFNSSEQVAEFVISRPLVIVGFFQDLEEEVAELFYDVIKDFPELTFGVITIGN
  VIGRFHVTLDSVLVFKKGKIVNRQKLINDSTNKQELNRVIKQHLTDFVIEYNTENKDLISELHIMSHMLL
  FVSKSSESYGIIIQHYKLASKEFQNKILFILVDADEPRNGRVFKYFRVTEVDIPSVQILNLSSDARYKMP
  SDDITYESLKKFGRSFLSKNATKHQSSEEIPKYWDQGLVKQLVGKNFNVVVFDKEKDVFVMFYAPWSKKC
  KMLFPLLEELGRKYQNHSTIIIAKIDVTANDIQLMYLDRYPFFRLFPSGSQQAVLYKGEHTLKGFSDFLE
  SHIKTKIEDEDELLSVEQNEVIEEEVLAEEKEVPMMRKGLPEQQSPELENMTKYVSKLEEPAGKKKTSEE
  VVVVVAKPKGPPVQKKKPKVKEEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_174924
• ORF Size: 1752 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_174924.1, NP_777584.1
• RefSeq Size: 2195
• RefSeq ORF: 1755
• Locus ID: 204474
• Protein Families: Druggable Genome
• MW: 66.7 kDa
• Gene Summary: This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has has an N-terminal ER-signal sequence, two thioredoxin (TRX) domains with non-classical Ser-Lys-Gln-Ser and Ser-Lys-Lys-Cys motifs, respectively, two TRX-like domains, and a C-terminal ER-retention sequence. The protein lacks oxidoreductase activity in vitro and probably functions as a chaperone. This gene's expression appears to be limited to the testis. [provided by RefSeq, Dec 2016]