RC74 (INTS9) (NM_018250) Human Tagged ORF Clone

CAT#: RC206837

INTS9 (Myc-DDK-tagged)-Human integrator complex subunit 9 (INTS9), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY413191) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206837 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified INTS9 protein (Cat# TP306837). The protein was produced from HEK293T cells transfected with INTS9 cDNA clone (Cat# RC206837) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: INTS9
• Synonyms: CPSF2L; INT9; RC74
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206837 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACTGTATTGCCTGTCAGGGCACCCAACCTTACCATGCAATGTGCTCAAATTCAAATCAACCACCA
  TTATGTTGGACTGCGGACTGGACATGACTTCTACCCTCAATTTCCTTCCTTTGCCACTTGTTCAAAGTCC
  CAGGCTGTCCAATCTTCCTGGCTGGTCCCTGAAGGATGGAAATGCTTTCTTGGACAAGGAGCTAAAGGAG
  TGCTCGGGTCATGTATTTGTGGATTCTGTGCCGGAATTCTGTTTACCAGAGACGGAGCTAATAGATCTGT
  CTACAGTAGATGTGATTCTCATCTCTAACTATCACTGTATGATGGCGCTGCCATACATCACCGAGCACAC
  CGGCTTCACAGGCACAGTGTATGCCACGGAACCCACCGTCCAGATCGGCAGGCTTCTCATGGAAGAGCTG
  GTGAATTTCATTGAAAGAGTGCCAAAGGCTCAGTCTGCCTCCTTGTGGAAGAATAAGGACATTCAGAGGC
  TGTTACCTTCTCCTCTCAAGGATGCAGTGGAAGTCTCAACCTGGAGAAGATGCTATACAATGCAAGAGGT
  GAACTCTGCCCTTAGTAAAATCCAGCTGGTGGGATATTCTCAGAAAATTGAGCTTTTTGGTGCGGTCCAG
  GTGACTCCTCTGAGCTCTGGCTATGCCCTTGGGAGCTCCAACTGGATCATCCAGTCTCATTACGAGAAAG
  TGTCTTATGTCTCTGGATCCTCCTTGCTTACCACACACCCCCAGCCCATGGACCAAGCTTCTCTCAAAAA
  CAGCGATGTTCTTGTTCTGACAGGGCTTACCCAGATCCCCACTGCAAACCCAGATGGAATGGTGGGAGAG
  TTCTGCAGCAACCTAGCTCTGACAGTCCGGAATGGAGGAAACGTGTTGGTTCCCTGCTACCCTTCTGGAG
  TGATCTATGACCTCCTGGAGTGCCTATATCAGTACATCGACTCAGCCGGGCTTTCCAGCGTCCCCCTCTA
  CTTCATCTCCCCTGTGGCCAACAGTTCACTGGAGTTTTCCCAGATCTTTGCTGAGTGGCTTTGTCACAAC
  AAACAGAGTAAGGTGTATCTTCCAGAACCACCTTTTCCTCATGCAGAGCTCATTCAGACCAATAAGCTGA
  AGCACTACCCCAGCATCCACGGAGACTTCAGCAACGACTTTAGACAGCCCTGTGTGGTGTTCACCGGGCA
  CCCTTCCCTCCGCTTCGGGGACGTGGTCCACTTCATGGAGCTCTGGGGAAAATCTAGTCTCAATACCGTC
  ATATTCACGGAACCAGACTTCTCCTACCTGGAAGCCCTGGCTCCTTACCAGCCGCTGGCCATGAAATGCA
  TCTACTGCCCCATCGACACCCGGCTGAACTTCATCCAGGTGTCAAAGCTGCTTAAAGAAGTGCAGCCCCT
  GCACGTGGTGTGTCCTGAGCAGTACACTCAGCCGCCCCCAGCCCAGTCCCACAGGATGGACCTCATGATC
  GACTGCCAGCCCCCCGCCATGTCCTATCGGCGGGCTGAGGTTCTCGCCCTGCCCTTCAAACGTCGGTACG
  AGAAGATCGAGATCATGCCAGAGCTCGCAGATTCACTGGTGCCCATGGAGATCAAGCCTGGCATCTCCTT
  GGCAACTGTCTCGGCCGTGCTGCACACCAAAGATAACAAGCACTTGCTTCAGCCCCCTCCTCGGCCCGCC
  CAGCCCACGAGCGGGAAGAAGAGAAAGCGGGTGAGCGATGACGTACCAGACTGCAAAGTCCTGAAGCCTT
  TGTTGAGCGGTTCCATCCCTGTGGAGCAGTTCGTGCAGACCCTGGAGAAGCATGGCTTCAGTGATATTAA
  GGTGGAGGACACAGCCAAGGGCCATATCGTCCTGCTCCAGGAGGCTGAGACGCTCATCCAGATTGAAGAA
  GACTCGACCCATATCATCTGCGACAATGACGAGATGCTCAGAGTGCGACTGCGGGACCTTGTCCTCAAAT
  TCTTACAGAAGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206837 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKLYCLSGHPTLPCNVLKFKSTTIMLDCGLDMTSTLNFLPLPLVQSPRLSNLPGWSLKDGNAFLDKELKE
  CSGHVFVDSVPEFCLPETELIDLSTVDVILISNYHCMMALPYITEHTGFTGTVYATEPTVQIGRLLMEEL
  VNFIERVPKAQSASLWKNKDIQRLLPSPLKDAVEVSTWRRCYTMQEVNSALSKIQLVGYSQKIELFGAVQ
  VTPLSSGYALGSSNWIIQSHYEKVSYVSGSSLLTTHPQPMDQASLKNSDVLVLTGLTQIPTANPDGMVGE
  FCSNLALTVRNGGNVLVPCYPSGVIYDLLECLYQYIDSAGLSSVPLYFISPVANSSLEFSQIFAEWLCHN
  KQSKVYLPEPPFPHAELIQTNKLKHYPSIHGDFSNDFRQPCVVFTGHPSLRFGDVVHFMELWGKSSLNTV
  IFTEPDFSYLEALAPYQPLAMKCIYCPIDTRLNFIQVSKLLKEVQPLHVVCPEQYTQPPPAQSHRMDLMI
  DCQPPAMSYRRAEVLALPFKRRYEKIEIMPELADSLVPMEIKPGISLATVSAVLHTKDNKHLLQPPPRPA
  QPTSGKKRKRVSDDVPDCKVLKPLLSGSIPVEQFVQTLEKHGFSDIKVEDTAKGHIVLLQEAETLIQIEE
  DSTHIICDNDEMLRVRLRDLVLKFLQKF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018250
• ORF Size: 1974 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018250.1, NM_018250.2, NM_018250.3, NP_060720.1
• RefSeq Size: 2764 bp
• RefSeq ORF: 1977 bp
• Locus ID: 55756
• MW: 73.8 kDa
• Gene Summary: This gene encodes a subunit of the Integrator complex. This protein complex binds the C-terminal domain of RNA polymerase II and likely plays a role in small nuclear RNA processing. The encoded protein has similarities to the subunits of the cleavage and polyadenylation specificity factor complex. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010]