DYNC1LI2 (NM_006141) Human Tagged ORF Clone

CAT#: RC206851

DYNC1LI2 (Myc-DDK-tagged)-Human dynein, cytoplasmic 1, light intermediate chain 2 (DYNC1LI2)

Product Images

Western blot validation of overexpression lysate (Cat# LY416842) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206851 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DYNC1LI2 protein (Cat# TP306851). The protein was produced from HEK293T cells transfected with DYNC1LI2 cDNA clone (Cat# RC206851) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DYNC1LI2
• Synonyms: DNCLI2; LIC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206851 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGGTGGGGGTGGAGAAGAAGCTGCTGCTAGGTCCCAACGGGCCCGCGGTGGCGGCCGCCGGCG
  ACCTGACCAGTGAGGAGGAGGAAGGCCAGAGCCTATGGTCCTCCATTCTGAGCGAAGTGTCCACCCGCGC
  CAGGTCCAAGCTGCCGTCCGGCAAGAACATCCTGGTCTTCGGTGAAGATGGTTCTGGTAAAACAACCCTC
  ATGACTAAACTACAAGGAGCTGAGCATGGCAAAAAAGGAAGAGGCCTAGAATATCTCTACCTCAGTGTCC
  ATGATGAGGACCGAGATGATCACACGCGCTGCAACGTGTGGATTCTGGATGGAGACTTGTACCACAAAGG
  CCTGCTGAAATTTGCAGTTTCTGCTGAATCCTTGCCAGAGACCCTCGTCATTTTTGTTGCAGACATGTCT
  AGACCTTGGACTGTGATGGAATCTCTGCAGAAATGGGCTAGTGTTTTACGTGAGCACATTGATAAAATGA
  AAATTCCACCAGAAAAAATGAGGGAGCTGGAACGGAAGTTTGTGAAAGATTTTCAAGACTATATGGAACC
  TGAAGAAGGTTGTCAAGGTTCCCCACAGAGAAGAGGCCCTCTGACCTCAGGCTCCGATGAAGAAAATGTT
  GCCCTGCCTCTGGGTGACAATGTGCTGACTCATAACCTGGGGATCCCGGTGTTGGTGGTGTGCACAAAGT
  GTGATGCGGTGAGTGTCCTGGAGAAGGAGCACGATTACAGGGATGAGCATTTGGACTTTATCCAGTCACA
  CCTGCGGAGGTTCTGCCTTCAGTATGGAGCTGCCTTGATTTACACATCAGTGAAAGAAGAGAAAAACCTC
  GACTTGTTGTATAAGTATATTGTTCATAAAACATACGGTTTCCACTTCACCACACCTGCCTTAGTTGTGG
  AAAAGGATGCCGTTTTTATACCTGCAGGCTGGGACAATGAAAAGAAAATAGCTATTTTACATGAAAATTT
  TACAACCGTGAAGCCGGAAGATGCATATGAAGACTTTATTGTGAAACCTCCCGTGAGAAAGCTGGTCCAC
  GACAAAGAGTTGGCAGCAGAAGATGAGCAGGTGTTCCTAATGAAGCAACAGTCACTCCTTGCCAAGCAAC
  CAGCCACTCCCACGAGAGCTTCTGAATCTCCTGCAAGAGGACCCTCTGGCTCTCCAAGGACCCAGGGTCG
  GGGAGGGCCAGCCAGTGTGCCTAGCTCCTCCCCAGGCACGTCAGTAAAAAAGCCAGACCCAAACATCAAA
  AATAATGCAGCAAGTGAAGGGGTGTTGGCCAGCTTCTTCAACAGTCTGTTGAGTAAAAAGACAGGCTCTC
  CTGGAAGTCCTGGTGCTGGTGGGGTGCAGAGCACAGCCAAGAACTCAGGACAAAAGACTGTGTTGTCAAA
  TGTTCAGGAAGAACTGGATAGAATGACTCGAAAGCCAGACTCTATGGTAACAAACTCTTCAACAGAAAAT
  GAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206851 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPVGVEKKLLLGPNGPAVAAAGDLTSEEEEGQSLWSSILSEVSTRARSKLPSGKNILVFGEDGSGKTTL
  MTKLQGAEHGKKGRGLEYLYLSVHDEDRDDHTRCNVWILDGDLYHKGLLKFAVSAESLPETLVIFVADMS
  RPWTVMESLQKWASVLREHIDKMKIPPEKMRELERKFVKDFQDYMEPEEGCQGSPQRRGPLTSGSDEENV
  ALPLGDNVLTHNLGIPVLVVCTKCDAVSVLEKEHDYRDEHLDFIQSHLRRFCLQYGAALIYTSVKEEKNL
  DLLYKYIVHKTYGFHFTTPALVVEKDAVFIPAGWDNEKKIAILHENFTTVKPEDAYEDFIVKPPVRKLVH
  DKELAAEDEQVFLMKQQSLLAKQPATPTRASESPARGPSGSPRTQGRGGPASVPSSSPGTSVKKPDPNIK
  NNAASEGVLASFFNSLLSKKTGSPGSPGAGGVQSTAKNSGQKTVLSNVQEELDRMTRKPDSMVTNSSTEN
  EA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006141
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006141.1, NM_006141.2, NP_006132.1
• RefSeq Size: 4352 bp
• RefSeq ORF: 1479 bp
• Locus ID: 1783
• Cytogenetics: 16q22.1
• Protein Families: Druggable Genome
• MW: 54.1 kDa
• Gene Summary: 'Cytoplasmic dynein is a microtubule-associated motor protein (Hughes et al., 1995 [PubMed 7738094]). See DYNC1H1 (MIM 600112) for general information about dyneins.[supplied by OMIM, Mar 2008]'