CSTF2T (NM_015235) Human Tagged ORF Clone

CAT#: RC206973

CSTF2T (Myc-DDK-tagged)-Human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, tau variant (CSTF2T)

Product Images

Western blot validation of overexpression lysate (Cat# LY414706) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206973 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CSTF2T protein (Cat# TP306973). The protein was produced from HEK293T cells transfected with CSTF2T cDNA clone (Cat# RC206973) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CSTF2T
• Synonyms: CstF-64T
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206973 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAGTTTGGCGGTGAGAGACCCGGCAATGGATCGATCACTGCGTTCCGTGTTCGTGGGGAACATTC
  CATATGAGGCAACTGAGGAGCAGTTAAAGGACATTTTCTCGGAGGTTGGTTCTGTTGTCAGTTTCCGGCT
  GGTATACGATAGAGAGACGGGAAAACCCAAGGGCTATGGCTTCTGCGAATACCAAGACCAGGAGACCGCG
  CTTAGTGCCATGCGGAACCTCAATGGGCGGGAGTTCAGTGGGAGAGCGCTTCGGGTGGACAATGCTGCCA
  GTGAAAAGAATAAGGAGGAGTTAAAGAGCCTCGGGCCTGCAGCGCCCATTATTGACTCACCCTATGGGGA
  TCCCATCGATCCAGAAGATGCCCCTGAATCGATTACCAGAGCAGTAGCCAGTCTCCCCCCGGAGCAGATG
  TTTGAGCTGATGAAGCAGATGAAGCTCTGTGTCCAAAACAGCCACCAGGAAGCTCGAAACATGTTACTTC
  AAAATCCACAACTGGCTTATGCACTGTTGCAGGCACAAGTAGTGATGAGAATCATGGATCCAGAGATTGC
  TCTGAAAATTCTGCATCGGAAGATACATGTCACACCACTGATCCCAGGCAAATCTCAGTCTGTGTCTGTC
  TCTGGCCCTGGCCCTGGCCCTGGCCCTGGGCTCTGCCCAGGACCTAATGTTCTGCTGAACCAGCAGAATC
  CTCCAGCTCCTCAGCCTCAGCATTTGGCTAGAAGACCTGTGAAGGACATTCCTCCTCTGATGCAGACTCC
  TATCCAGGGTGGAATTCCAGCTCCAGGGCCAATACCAGCTGCAGTTCCCGGAGCTGGTCCTGGTTCCTTA
  ACTCCTGGAGGAGCAATGCAGCCCCAACTTGGAATGCCAGGGGTTGGCCCAGTGCCTTTAGAGCGGGGAC
  AAGTGCAGATGTCAGATCCTAGAGCTCCTATACCTCGCGGACCCGTGACTCCTGGTGGTCTGCCTCCTCG
  AGGACTGTTAGGAGATGCTCCAAATGACCCACGTGGAGGGACTTTGCTTTCAGTCACTGGAGAAGTGGAG
  CCCAGAGGTTATCTGGGTCCACCCCATCAGGGTCCCCCCATGCATCATGCCTCTGGTCATGACACTCGTG
  GCCCTTCCTCACATGAGATGAGGGGAGGGCCATTAGGAGATCCCAGACTGCTAATTGGAGAGCCCAGAGG
  CCCCATGATAGATCAAAGGGGTCTACCTATGGATGGTAGAGGTGGTAGAGATTCTCGAGCGATGGAGACT
  CGCGCCATGGAAACTGAGGTCTTAGAGACACGTGTAATGGAGAGGAGAGGAATGGAGACCTGTGCGATGG
  AAACCAGAGGGATGGAAGCAAGGGGCATGGATGCAAGAGGATTGGAGATGAGGGGCCCTGTCCCCAGTTC
  AAGAGGCCCTATGACTGGTGGAATTCAGGGTCCTGGTCCCATTAATATAGGGGCAGGTGGCCCTCCTCAG
  GGACCCAGACAGGTCCCAGGCATTTCAGGGGTGGGGAATCCTGGAGCTGGTATGCAGGGTACAGGCATAC
  AAGGAACAGGCATGCAGGGAGCAGGCATACAAGGAGGAGGGATGCAGGGGGCAGGCATACAAGGAGTCAG
  TATACAAGGAGGAGGTATACAAGGAGGAGGTATACAGGGGGCAAGCAGGCAAGGTGGAAGCCAGCCTAGC
  AGTTTTAGTCCTGGGCAGAGCCAGGTCACTCCACAGGATCAGGAGAAGGCAGCTTTGATCATGCAGGTTC
  TTCAACTGACTGCAGATCAGATTGCCATGCTGCCCCCTGAGCAAAGGCAGAGTATCCTGATTTTAAAGGA
  ACAAATCCAGAAATCCACTGGAGCGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206973 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSLAVRDPAMDRSLRSVFVGNIPYEATEEQLKDIFSEVGSVVSFRLVYDRETGKPKGYGFCEYQDQETA
  LSAMRNLNGREFSGRALRVDNAASEKNKEELKSLGPAAPIIDSPYGDPIDPEDAPESITRAVASLPPEQM
  FELMKQMKLCVQNSHQEARNMLLQNPQLAYALLQAQVVMRIMDPEIALKILHRKIHVTPLIPGKSQSVSV
  SGPGPGPGPGLCPGPNVLLNQQNPPAPQPQHLARRPVKDIPPLMQTPIQGGIPAPGPIPAAVPGAGPGSL
  TPGGAMQPQLGMPGVGPVPLERGQVQMSDPRAPIPRGPVTPGGLPPRGLLGDAPNDPRGGTLLSVTGEVE
  PRGYLGPPHQGPPMHHASGHDTRGPSSHEMRGGPLGDPRLLIGEPRGPMIDQRGLPMDGRGGRDSRAMET
  RAMETEVLETRVMERRGMETCAMETRGMEARGMDARGLEMRGPVPSSRGPMTGGIQGPGPINIGAGGPPQ
  GPRQVPGISGVGNPGAGMQGTGIQGTGMQGAGIQGGGMQGAGIQGVSIQGGGIQGGGIQGASRQGGSQPS
  SFSPGQSQVTPQDQEKAALIMQVLQLTADQIAMLPPEQRQSILILKEQIQKSTGAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015235
• ORF Size: 1848 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015235.1, NM_015235.2, NP_056050.1
• RefSeq Size: 4127
• RefSeq ORF: 1851
• Locus ID: 23283
• MW: 64.5 kDa