TCCR (IL27RA) (NM_004843) Human Tagged ORF Clone

CAT#: RC207012

IL27RA (Myc-DDK-tagged)-Human interleukin 27 receptor, alpha (IL27RA)

Product Images

Western blot validation of overexpression lysate (Cat# LY417709) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207012 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified IL27RA protein (Cat# TP307012). The protein was produced from HEK293T cells transfected with IL27RA cDNA clone (Cat# RC207012) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IL27RA
• Synonyms: CRL1; IL-27RA; IL27R; TCCR; WSX1; zcytor1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207012 representing NM_004843
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGGAGGCAGGGGCGCCCCTTTCTGGCTGTGGCCGCTGCCCAAGCTGGCGCTGCTGCCTCTGTTGT
  GGGTGCTTTTCCAGCGGACGCGTCCCCAGGGCAGCGCCGGGCCACTGCAGTGCTACGGAGTTGGACCCTT
  GGGCGACTTGAACTGCTCGTGGGAGCCTCTTGGGGACCTGGGAGCCCCCTCCGAGTTACACCTCCAGAGC
  CAAAAGTACCGTTCCAACAAAACCCAGACTGTGGCAGTGGCAGCCGGACGGAGCTGGGTGGCCATTCCTC
  GGGAACAGCTCACCATGTCTGACAAACTCCTTGTCTGGGGCACTAAGGCAGGCCAGCCTCTCTGGCCCCC
  CGTCTTCGTGAACCTAGAAACCCAAATGAAGCCAAACGCCCCCCGGCTGGGCCCTGACGTGGACTTTTCC
  GAGGATGACCCCCTGGAGGCCACTGTCCATTGGGCCCCACCTACATGGCCATCTCATAAAGTTCTGATCT
  GCCAGTTCCACTACCGAAGATGTCAGGAGGCGGCCTGGACCCTGCTGGAACCGGAGCTGAAGACCATACC
  CCTGACCCCTGTTGAGATCCAAGATTTGGAGCTAGCCACTGGCTACAAAGTGTATGGCCGCTGCCGGATG
  GAGAAAGAAGAGGATTTGTGGGGCGAGTGGAGCCCCATTTTGTCCTTCCAGACACCGCCTTCTGCTCCAA
  AAGATGTGTGGGTATCAGGGAACCTCTGTGGGACGCCTGGAGGAGAGGAACCTTTGCTTCTATGGAAGGC
  CCCAGGGCCCTGTGTGCAGGTGAGCTACAAAGTCTGGTTCTGGGTTGGAGGTCGTGAGCTGAGTCCAGAA
  GGAATTACCTGCTGCTGCTCCCTAATTCCCAGTGGGGCGGAGTGGGCCAGGGTGTCCGCTGTCAACGCCA
  CAAGCTGGGAGCCTCTCACCAACCTCTCTTTGGTCTGCTTGGATTCAGCCTCTGCCCCCCGTAGCGTGGC
  AGTCAGCAGCATCGCTGGGAGCACGGAGCTACTGGTGACCTGGCAACCGGGGCCTGGGGAACCACTGGAG
  CATGTAGTGGACTGGGCTCGAGATGGGGACCCCCTGGAGAAACTCAACTGGGTCCGGCTTCCCCCTGGGA
  ACCTCAGTGCTCTGTTACCAGGGAATTTCACTGTCGGGGTCCCCTATCGAATCACTGTGACCGCAGTCTC
  TGCTTCAGGCTTGGCCTCTGCATCCTCCGTCTGGGGGTTCAGGGAGGAATTAGCACCCCTAGTGGGGCCA
  ACGCTTTGGCGACTCCAAGATGCCCCTCCAGGGACCCCCGCCATAGCGTGGGGAGAGGTCCCAAGGCACC
  AGCTTCGAGGCCACCTCACCCACTACACCTTGTGTGCACAGAGTGGAACCAGCCCCTCCGTCTGCATGAA
  TGTGAGTGGCAACACACAGAGTGTCACCCTGCCTGACCTTCCTTGGGGTCCCTGTGAGCTGTGGGTGACA
  GCATCTACCATCGCTGGACAGGGCCCTCCTGGTCCCATCCTCCGGCTTCATCTACCAGATAACACCCTGA
  GGTGGAAAGTTCTGCCGGGCATCCTATTCTTGTGGGGCTTGTTCCTGTTGGGGTGTGGCCTGAGCCTGGC
  CACCTCTGGAAGGTGCTACCACCTAAGGCACAAAGTGCTGCCCCGCTGGGTCTGGGAGAAAGTTCCTGAT
  CCTGCCAACAGCAGTTCAGGCCAGCCCCACATGGAGCAAGTACCTGAGGCCCAGCCCCTTGGGGACTTGC
  CCATCCTGGAAGTGGAGGAGATGGAGCCCCCGCCGGTTATGGAGTCCTCCCAGCCCGCCCAGGCCACCGC
  CCCGCTTGACTCTGGGTATGAGAAGCACTTCCTGCCCACACCTGAGGAGCTGGGCCTTCTGGGGCCCCCC
  AGGCCACAGGTTCTGGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207012 representing NM_004843
  Red=Cloning site Green=Tags(s)
  
  MRGGRGAPFWLWPLPKLALLPLLWVLFQRTRPQGSAGPLQCYGVGPLGDLNCSWEPLGDLGAPSELHLQS
  QKYRSNKTQTVAVAAGRSWVAIPREQLTMSDKLLVWGTKAGQPLWPPVFVNLETQMKPNAPRLGPDVDFS
  EDDPLEATVHWAPPTWPSHKVLICQFHYRRCQEAAWTLLEPELKTIPLTPVEIQDLELATGYKVYGRCRM
  EKEEDLWGEWSPILSFQTPPSAPKDVWVSGNLCGTPGGEEPLLLWKAPGPCVQVSYKVWFWVGGRELSPE
  GITCCCSLIPSGAEWARVSAVNATSWEPLTNLSLVCLDSASAPRSVAVSSIAGSTELLVTWQPGPGEPLE
  HVVDWARDGDPLEKLNWVRLPPGNLSALLPGNFTVGVPYRITVTAVSASGLASASSVWGFREELAPLVGP
  TLWRLQDAPPGTPAIAWGEVPRHQLRGHLTHYTLCAQSGTSPSVCMNVSGNTQSVTLPDLPWGPCELWVT
  ASTIAGQGPPGPILRLHLPDNTLRWKVLPGILFLWGLFLLGCGLSLATSGRCYHLRHKVLPRWVWEKVPD
  PANSSSGQPHMEQVPEAQPLGDLPILEVEEMEPPPVMESSQPAQATAPLDSGYEKHFLPTPEELGLLGPP
  RPQVLA
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004843
• ORF Size: 1908 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004843.1, NM_004843.2, NM_004843.3, NP_004834.1
• RefSeq Size: 3258 bp
• RefSeq ORF: 1911 bp
• Locus ID: 9466
• Domains: FN3
• Protein Families: Druggable Genome, Transmembrane
• MW: 69.3 kDa
• Gene Summary: In mice, CD4+ helper T-cells differentiate into type 1 (Th1) cells, which are critical for cell-mediated immunity, predominantly under the influence of IL12. Also, IL4 influences their differentiation into type 2 (Th2) cells, which are critical for most antibody responses. Mice deficient in these cytokines, their receptors, or associated transcription factors have impaired, but are not absent of, Th1 or Th2 immune responses. This gene encodes a protein which is similar to the mouse T-cell cytokine receptor Tccr at the amino acid level, and is predicted to be a glycosylated transmembrane protein. [provided by RefSeq, Jul 2008]