BRINP2 (NM_021165) Human Tagged ORF Clone

CAT#: RC207049

FAM5B (Myc-DDK-tagged)-Human family with sequence similarity 5, member B (FAM5B)

Product Images

Western blot validation of overexpression lysate (Cat# LY412054) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207049 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRINP2
• Synonyms: DBCCR1L2; FAM5B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207049 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTGGCAGTGTGGCACTCGGTTTAGAGGGCTTCGGCCGGCGGTGGCCCCATGGACAGCCCTGCTGG
  CACTGGGCCTGCCTGGCTGGGTGTTGGCTGTCTCAGCCACGGCGGCTGCTGTGGTCCCCGAGCAGCATGC
  CTCCGTAGCTGGCCAGCATCCCCTGGACTGGCTGCTCACAGACCGGGGCCCCTTCCACCGCGCTCAGGAG
  TATGCTGACTTCATGGAGCGGTACCGCCAGGGTTTCACCACCAGGTACAGGATTTATAGGGAGTTTGCCC
  GTTGGAAGGTGAACAACTTGGCTCTGGAAAGGAAGGACTTCTTCAGTTTGCCATTGCCTCTTGCCCCAGA
  GTTTATCCGGAACATTCGCCTCCTTGGAAGGAGACCCAATCTGCAACAGGTTACAGAAAATCTGATTAAA
  AAGTACGGCACTCATTTCTTACTTTCTGCCACCCTTGGAGGAGAAGAGTCCCTGACCATTTTTGTGGACA
  AGCAGAAACTGGGAAGAAAGACAGAGACAACAGGAGGTGCCTCTATAATCGGGGGCAGTGGGAACAGCAC
  AGCTGTGTCCCTGGAGACCCTGCACCAGCTGGCCGCCTCCTACTTCATCGACAGAGAGAGCACGCTGCGA
  CGGCTGCACCATATCCAGATAGCCACGGGGGCCATCAAGGTCACCGAGACCAGGACCGGTCCTCTGGGCT
  GCAGCAACTATGACAATCTGGACTCAGTCAGTTCTGTCTTGGTACAGAGTCCAGAGAACAAAGTACAGTT
  ACTTGGCCTTCAGGTGCTGCTGCCTGAGTATCTGCGTGAGCGCTTTGTAGCTGCAGCACTCAGCTACATC
  ACATGCAGCTCTGAGGGTGAGCTCGTCTGCAAGGAGAATGACTGCTGGTGCAAGTGCAGCCCCACCTTCC
  CTGAATGCAACTGCCCTGATGCTGACATCCAGGCCATGGAGGACAGCCTGCTGCAGATCCAGGACTCCTG
  GGCCACTCACAACCGGCAGTTTGAAGAGTCAGAAGAGTTCCAGGCCCTGCTGAAAAGGCTGCCCGATGAC
  CGGTTCCTGAACTCCACAGCTATCTCCCAGTTCTGGGCCATGGACACCAGCCTTCAGCACCGCTACCAGC
  AGCTGGGAGCTGGCTTGAAAGTGCTGTTCAAAAAGACCCATCGGATCCTACGCCGGCTCTTCAACCTCTG
  CAAGCGCTGCCATCGCCAGCCTCGCTTCCGCCTGCCCAAGGAGAGGTCCTTGTCCTACTGGTGGAACCGA
  ATCCAGTCCCTCCTCTACTGTGGGGAAAGCACCTTTCCTGGCACTTTCCTGGAACAGAGCCACAGCTGCA
  CCTGCCCCTATGACCAATCTTCCTGCCAGGGCCCCATCCCATGTGCCTTGGGCGAAGGGCCCGCGTGTGC
  CCACTGTGCTCCAGACAATAGCACACGCTGTGGGAGCTGCAACCCGGGCTATGTGCTGGCCCAGGGGCTG
  TGCCGGCCAGAGGTGGCCGAGTCCCTGGAAAACTTTCTTGGGCTGGAGACAGACTTGCAGGACCTGGAGC
  TAAAGTACCTGCTGCAGAAGCAGGATAGCCGCATTGAGGTACACTCCATCTTCATCAGCAATGACATGCG
  GCTGGGCAGCTGGTTTGACCCTTCCTGGAGGAAGCGCATGCTGCTCACCCTGAAGAGCAACAAGTACAAG
  CCTGGGCTGGTGCACGTGATGTTGGCCTTGTCCTTGCAGATCTGTCTCACCAAGAACAGCACCCTGGAGC
  CTGTCATGGCCATCTACGTCAACCCCTTTGGGGGCAGCCACTCTGAGAGCTGGTTCATGCCTGTGAATGA
  GGGCAGCTTTCCTGACTGGGAAAGGACTAACGTGGATGCAGCTGCCCAGTGCCAAAACTGGACTATCACC
  TTGGGGAATAGGTGGAAGACTTTCTTTGAGACAGTTCATGTTTACCTACGGAGCCGAATCAAGTCCCTGG
  ATGACAGCTCCAATGAGACAATCTACTATGAGCCCCTGGAGATGACTGATCCCTCTAAGAATTTGGGTTA
  CATGAAAATTAACACCTTGCAGGTCTTTGGCTACAGCCTGCCCTTTGACCCAGATGCTATCCGGGACTTA
  ATTCTCCAGTTGGACTACCCATATACTCAAGGTTCCCAGGACTCTGCACTCTTGCAGCTCATTGAGCTCA
  GGGACCGGGTGAACCAGCTTTCTCCACCTGGCAAAGTCCGACTTGACCTTTTCTCCTGCTTGCTCCGGCA
  TCGGCTTAAGCTGGCCAACAATGAGGTGGGCAGGATCCAGTCCTCCCTGAGGGCTTTCAATTCTAAGCTG
  CCAAACCCTGTGGAATATGAGACCGGCAAACTCTGTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207049 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRWQCGTRFRGLRPAVAPWTALLALGLPGWVLAVSATAAAVVPEQHASVAGQHPLDWLLTDRGPFHRAQE
  YADFMERYRQGFTTRYRIYREFARWKVNNLALERKDFFSLPLPLAPEFIRNIRLLGRRPNLQQVTENLIK
  KYGTHFLLSATLGGEESLTIFVDKQKLGRKTETTGGASIIGGSGNSTAVSLETLHQLAASYFIDRESTLR
  RLHHIQIATGAIKVTETRTGPLGCSNYDNLDSVSSVLVQSPENKVQLLGLQVLLPEYLRERFVAAALSYI
  TCSSEGELVCKENDCWCKCSPTFPECNCPDADIQAMEDSLLQIQDSWATHNRQFEESEEFQALLKRLPDD
  RFLNSTAISQFWAMDTSLQHRYQQLGAGLKVLFKKTHRILRRLFNLCKRCHRQPRFRLPKERSLSYWWNR
  IQSLLYCGESTFPGTFLEQSHSCTCPYDQSSCQGPIPCALGEGPACAHCAPDNSTRCGSCNPGYVLAQGL
  CRPEVAESLENFLGLETDLQDLELKYLLQKQDSRIEVHSIFISNDMRLGSWFDPSWRKRMLLTLKSNKYK
  PGLVHVMLALSLQICLTKNSTLEPVMAIYVNPFGGSHSESWFMPVNEGSFPDWERTNVDAAAQCQNWTIT
  LGNRWKTFFETVHVYLRSRIKSLDDSSNETIYYEPLEMTDPSKNLGYMKINTLQVFGYSLPFDPDAIRDL
  ILQLDYPYTQGSQDSALLQLIELRDRVNQLSPPGKVRLDLFSCLLRHRLKLANNEVGRIQSSLRAFNSKL
  PNPVEYETGKLCS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021165
• ORF Size: 2349 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021165.1, NM_021165.2, NM_021165.3, NP_066988.1
• RefSeq Size: 3617 bp
• RefSeq ORF: 2352 bp
• Locus ID: 57795
• Domains: MACPF
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• MW: 89 kDa