SLC25A34 (NM_207348) Human Tagged ORF Clone

CAT#: RC207061

SLC25A34 (Myc-DDK-tagged)-Human solute carrier family 25, member 34 (SLC25A34)

Product Images

Western blot validation of overexpression lysate (Cat# LY403973) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207061 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC25A34
• Synonyms: RP11-169K16.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207061 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACGGTGCCCCCAGCAGTGGACCTGGTGCTGGGTGCTTCTGCCTGCTGCCTGGCCTGTGTCTTCA
  CCAACCCCCTGGAGGTGGTGAAGACGCGGCTGCAGCTGCAGGGGGAGCTGCAGGCCCGGGGCACCTACCC
  ACGGCCCTACCATGGCTTCATAGCCTCTGTCGCTGCTGTGGCCCGAGCAGACGGGCTGTGGGGCCTGCAG
  AAGGGGCTGGCTGCCGGCCTTCTGTACCAAGGCCTCATGAATGGCGTTCGTTTCTACTGCTACAGCCTGG
  CGTGCCAGGCTGGCCTCACGCAGCAACCAGGTGGCACCGTGGTTGCGGGAGCCGTGGCGGGGGCACTGGG
  AGCCTTCGTGGGGAGCCCTGCTTACCTGATCAAAACGCAGCTGCAAGCTCAGACAGTGGCCGCAGTGGCC
  GTGGGACACCAGCACAATCACCAGACTGTCCTGGGTGCCTTGGAGACCATCTGGCGGCAGCAAGGGCTCT
  TGGGGCTGTGGCAGGGCGTTGGTGGGGCTGTGCCCCGAGTCATGGTGGGCTCAGCTGCCCAGCTGGCCAC
  CTTCGCCTCTGCCAAGGCCTGGGTACAGAAGCAACAGTGGCTCCCTGAGGACAGCTGGCTGGTGGCCCTG
  GCTGGGGGCATGATCAGCAGCATAGCCGTGGTTGTCGTCATGACTCCCTTCGATGTGGTCAGCACGCGGC
  TATACAATCAGCCGGTGGACACAGCTGGCAGGGGCCAGCTCTATGGGGGCCTCACCGACTGCATGGTGAA
  GATCTGGCGGCAGGAGGGCCCCCTGGCACTCTACAAGGGCCTGGGCCCCGCCTACCTGCGCCTGGGCCCC
  CACACCATCCTCAGCATGCTCTTCTGGGACGAGCTTCGGAAACTGGCTGGGCGGGCCCAGCACAAGGGCA
  CC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207061 protein sequence
  Red=Cloning site Green=Tags(s)
  
  METVPPAVDLVLGASACCLACVFTNPLEVVKTRLQLQGELQARGTYPRPYHGFIASVAAVARADGLWGLQ
  KGLAAGLLYQGLMNGVRFYCYSLACQAGLTQQPGGTVVAGAVAGALGAFVGSPAYLIKTQLQAQTVAAVA
  VGHQHNHQTVLGALETIWRQQGLLGLWQGVGGAVPRVMVGSAAQLATFASAKAWVQKQQWLPEDSWLVAL
  AGGMISSIAVVVVMTPFDVVSTRLYNQPVDTAGRGQLYGGLTDCMVKIWRQEGPLALYKGLGPAYLRLGP
  HTILSMLFWDELRKLAGRAQHKGT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207348
• ORF Size: 912 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207348.1, NM_207348.2, NP_997231.1
• RefSeq Size: 3172 bp
• RefSeq ORF: 915 bp
• Locus ID: 284723
• Protein Families: Transmembrane
• MW: 32.2 kDa
• Gene Summary: SLC25A34 belongs to the SLC25 family of mitochondrial carrier proteins (Haitina et al., 2006 [PubMed 16949250]). [supplied by OMIM, Mar 2008]