CYP51A1 (NM_000786) Human Tagged ORF Clone

CAT#: RC207078

CYP51A1 (Myc-DDK-tagged)-Human cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 1

Product Images

Coomassie blue staining of purified CYP51A1 protein (Cat# TP307078). The protein was produced from HEK293T cells transfected with CYP51A1 cDNA clone (Cat# RC207078) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CYP51A1
• Synonyms: CP51; CYP51; CYPL1; LDM; P450-14DM; P450L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207078 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCTGGGATGCTGCTGCTGGGCTTGCTGCAGGCGGGTGGGTCGGTGCTGGGCCAGGCGA
  TGGAGAAGGTGACAGGCGGCAACCTCTTGTCCATGCTGCTGATCGCCTGCGCCTTCACCCTCAGCCTGGT
  CTACCTGATCCGTCTGGCCGCCGGCCACCTGGTCCAGCTGCCCGCAGGGGTGAAAAGTCCTCCATACATT
  TTCTCCCCAATTCCATTCCTTGGGCATGCCATAGCATTTGGGAAAAGTCCAATTGAATTTCTAGAAAATG
  CATATGAGAAGTATGGACCTGTATTTAGTTTTACCATGGTAGGCAAGACATTTACTTACCTTCTGGGGAG
  TGATGCTGCTGCACTGCTTTTTAATAGTAAAAATGAAGACCTGAATGCAGAAGATGTCTACAGTCGCCTG
  ACAACACCTGTGTTTGGGAAGGGAGTTGCATACGATGTGCCTAATCCAGTTTTCTTGGAGCAGAAGAAAA
  TGTTAAAAAGTGGCCTTAACATAGCCCACTTTAAACAGCATGTTTCTATAATTGAAAAAGAAACAAAGGA
  ATACTTTGAGAGTTGGGGAGAAAGTGGAGAAAAAAATGTGTTTGAAGCTCTTTCTGAGCTCATAATTTTA
  ACAGCTAGCCATTGTTTGCATGGAAAGGAAATCAGAAGTCAACTCAATGAAAAGGTAGCACAGCTGTATG
  CAGATTTGGATGGAGGTTTCAGCCATGCAGCCTGGCTCTTACCAGGTTGGCTGCCTTTGCCTAGTTTCAG
  ACGCAGGGACAGAGCTCATCGGGAAATCAAGGATATTTTCTATAAGGCAATCCAGAAACGCAGACAGTCT
  CAAGAAAAAATTGATGACATTCTCCAAACTTTACTAGATGCTACATACAAGGATGGGCGTCCTTTGACTG
  ATGATGAAGTAGCAGGGATGCTTATTGGATTACTCTTGGCAGGGCAGCATACATCCTCAACTACTAGTGC
  TTGGATGGGCTTCTTTTTGGCCAGAGACAAAACACTTCAAAAAAAATGTTATTTAGAACAGAAAACAGTC
  TGTGGAGAGAATCTGCCTCCTTTAACTTATGACCAGCTCAAGGATCTAAATTTACTTGATCGCTGTATAA
  AAGAAACATTAAGACTTAGACCTCCTGTAATGATCATGATGAGAATGGCCAGAACTCCTCAGACTGTGGC
  AGGGTATACCATTCCTCCAGGACATCAGGTGTGTGTTTCTCCCACTGTCAATCAAAGACTTAAAGACTCA
  TGGGTAGAACGCCTGGACTTTAATCCTGATCGCTACTTACAGGATAACCCAGCATCAGGGGAAAAGTTTG
  CCTATGTGCCATTTGGAGCTGGGCGTCATCGTTGTATTGGGGAAAATTTTGCCTATGTTCAAATTAAGAC
  AATTTGGTCCACTATGCTTCGTTTATATGAATTTGATCTCATTGATGGATACTTTCCCACTGTGAATTAT
  ACAACTATGATTCACACCCCTGAAAACCCAGTTATCCGTTACAAACGAAGATCAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207078 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAAGMLLLGLLQAGGSVLGQAMEKVTGGNLLSMLLIACAFTLSLVYLIRLAAGHLVQLPAGVKSPPYI
  FSPIPFLGHAIAFGKSPIEFLENAYEKYGPVFSFTMVGKTFTYLLGSDAAALLFNSKNEDLNAEDVYSRL
  TTPVFGKGVAYDVPNPVFLEQKKMLKSGLNIAHFKQHVSIIEKETKEYFESWGESGEKNVFEALSELIIL
  TASHCLHGKEIRSQLNEKVAQLYADLDGGFSHAAWLLPGWLPLPSFRRRDRAHREIKDIFYKAIQKRRQS
  QEKIDDILQTLLDATYKDGRPLTDDEVAGMLIGLLLAGQHTSSTTSAWMGFFLARDKTLQKKCYLEQKTV
  CGENLPPLTYDQLKDLNLLDRCIKETLRLRPPVMIMMRMARTPQTVAGYTIPPGHQVCVSPTVNQRLKDS
  WVERLDFNPDRYLQDNPASGEKFAYVPFGAGRHRCIGENFAYVQIKTIWSTMLRLYEFDLIDGYFPTVNY
  TTMIHTPENPVIRYKRRSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000786
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000786.1, NM_000786.2, NM_000786.3, NP_000777.1
• RefSeq Size: 3208 bp
• RefSeq ORF: 1530 bp
• Locus ID: 1595
• Cytogenetics: 7q21.2
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• MW: 57.3 kDa
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]'