AADAC (NM_001086) Human Tagged ORF Clone

CAT#: RC207111

AADAC (Myc-DDK-tagged)-Human arylacetamide deacetylase (esterase) (AADAC)

Product Images

Western blot validation of overexpression lysate (Cat# LY421330) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207111 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AADAC protein (Cat# TP307111). The protein was produced from HEK293T cells transfected with AADAC cDNA clone (Cat# RC207111) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AADAC
• Synonyms: CES5A1; DAC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207111 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAGAAAATCGCTGTACCTTCTGATTGTGGGGATCCTCATAGCATATTATATTTATACGCCTCTCC
  CAGATAACGTTGAGGAGCCATGGAGAATGATGTGGATAAACGCACATCTGAAAACTATACAAAATTTGGC
  TACATTTGTGGAGCTCCTGGGACTTCACCATTTTATGGATTCCTTTAAGGTTGTCGGGAGCTTTGATGAA
  GTCCCACCAACCTCAGATGAAAATGTCACTGTGACTGAGACAAAATTCAACAACATTCTTGTTCGGGTAT
  ATGTGCCAAAGAGAAAGTCTGAAGCACTAAGAAGGGGGTTGTTTTACATCCATGGTGGAGGCTGGTGCGT
  GGGAAGTGCTGCTCTAAGTGGTTATGACTTGCTGTCAAGATGGACAGCAGACAGACTTGATGCTGTCGTC
  GTATCAACCAACTACAGATTAGCACCTAAGTATCATTTCCCAATTCAATTTGAAGATGTATATAATGCCT
  TAAGGTGGTTCTTACGTAAAAAAGTTCTTGCAAAATATGGTGTGAACCCTGAGAGAATCGGTATTTCTGG
  AGATAGTGCAGGAGGGAATTTAGCTGCAGCAGTGACTCAACAGCTCCTTGATGACCCAGATGTCAAGATC
  AAACTCAAGATCCAGTCTTTAATTTATCCTGCCCTTCAGCCTCTTGATGTAGATTTACCGTCATATCAAG
  AAAATTCAAATTTTCTATTTCTATCCAAATCACTCATGGTCAGATTCTGGAGTGAATATTTTACCACTGA
  TAGATCACTTGAAAAAGCCATGCTTTCCAGACAACATGTACCTGTGGAATCAAGTCATCTCTTCAAATTT
  GTTAATTGGAGTTCCCTGCTCCCTGAGAGGTTTATAAAAGGACATGTTTATAACAATCCAAATTATGGCA
  GTTCTGAGCTGGCTAAAAAATATCCAGGGTTCCTAGATGTGAGGGCAGCCCCTTTGTTGGCTGATGACAA
  CAAATTACGTGGCTTACCCCTGACCTATGTCATCACCTGTCAATATGATCTCTTAAGAGATGATGGACTC
  ATGTATGTCACCCGACTTCGCAACACTGGGGTTCAGGTGACTCATAACCATGTTGAGGATGGATTCCATG
  GAGCATTTTCATTTCTGGGACTTAAAATTAGTCACAGACTTATAAATCAGTATATTGAGTGGCTAAAGGA
  AAATCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207111 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGRKSLYLLIVGILIAYYIYTPLPDNVEEPWRMMWINAHLKTIQNLATFVELLGLHHFMDSFKVVGSFDE
  VPPTSDENVTVTETKFNNILVRVYVPKRKSEALRRGLFYIHGGGWCVGSAALSGYDLLSRWTADRLDAVV
  VSTNYRLAPKYHFPIQFEDVYNALRWFLRKKVLAKYGVNPERIGISGDSAGGNLAAAVTQQLLDDPDVKI
  KLKIQSLIYPALQPLDVDLPSYQENSNFLFLSKSLMVRFWSEYFTTDRSLEKAMLSRQHVPVESSHLFKF
  VNWSSLLPERFIKGHVYNNPNYGSSELAKKYPGFLDVRAAPLLADDNKLRGLPLTYVITCQYDLLRDDGL
  MYVTRLRNTGVQVTHNHVEDGFHGAFSFLGLKISHRLINQYIEWLKENL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001086
• ORF Size: 1197 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001086.1, NM_001086.2, NP_001077.2
• RefSeq Size: 1725 bp
• RefSeq ORF: 1200 bp
• Locus ID: 13
• Protein Families: Druggable Genome, Transmembrane
• MW: 45.7 kDa
• Gene Summary: Microsomal arylacetamide deacetylase competes against the activity of cytosolic arylamine N-acetyltransferase, which catalyzes one of the initial biotransformation pathways for arylamine and heterocyclic amine carcinogens [provided by RefSeq, Jul 2008]