GPR6 (NM_005284) Human Tagged ORF Clone

CAT#: RC207204

GPR6 (Myc-DDK-tagged)-Human G protein-coupled receptor 6 (GPR6)

Product Images

Western blot validation of overexpression lysate (Cat# LY401626) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207204 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPR6
• Synonyms: G protein-coupled receptor 6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207204 representing NM_005284
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGCGAGCGCCGCCTCGCTCAACGACTCCCAGGTGGTGGTAGTGGCGGCCGAAGGAGCGGCGGCGG
  CGGCCACAGCAGCAGGGGGGCCGGACACGGGCGAATGGGGACCCCCTGCTGCGGCGGCTCTAGGAGCCGG
  CGGCGGAGCTAATGGGTCTCTGGAGCTGTCCTCGCAGCTGTCGGCTGGGCCACCGGGACTCCTGCTGCCA
  GCGGTGAATCCGTGGGACGTGCTCCTGTGCGTGTCGGGGACAGTGATCGCTGGAGAAAACGCGCTGGTGG
  TGGCGCTCATCGCGTCCACTCCGGCGCTGCGCACGCCCATGTTCGTGCTGGTAGGCAGCCTGGCCACCGC
  TGACCTGTTGGCGGGCTGTGGCCTCATCTTGCACTTTGTGTTCCAGTACTTGGTGCCCTCGGAGACTGTG
  AGTCTGCTCACGGTGGGCTTCCTCGTGGCCTCCTTCGCCGCCTCTGTCAGCAGCCTGCTGGCCATTACGG
  TGGACCGCTACCTGTCCCTGTATAACGCGCTCACCTATTACTCGCGCCGGACCCTGTTGGGCGTGCACCT
  CCTGCTTGCCGCCACTTGGACCGTGTCCCTAGGCCTGGGGCTGCTGCCCGTGCTGGGCTGGAACTGCCTG
  GCAGAGCGCGCCGCCTGCAGCGTGGTGCGCCCGCTGGCGCGCAGCCACGTGGCTCTGCTCTCCGCCGCCT
  TCTTCATGGTCTTCGGCATCATGCTGCACCTGTACGTGCGCATCTGCCAGGTGGTCTGGCGCCACGCGCA
  CCAGATCGCGCTGCAGCAGCACTGCCTGGCGCCACCCCATCTCGCTGCCACCAGAAAGGGTGTGGGTACA
  CTGGCTGTGGTGCTGGGCACTTTCGGCGCCAGCTGGCTGCCCTTCGCCATCTATTGCGTGGTGGGCAGCC
  ATGAGGACCCGGCGGTCTACACTTACGCCACCCTGCTGCCCGCCACCTACAACTCCATGATCAATCCCAT
  CATCTATGCCTTCCGCAACCAGGAGATCCAGCGCGCCCTGTGGCTCCTGCTCTGTGGCTGTTTCCAGTCC
  AAAGTGCCCTTTCGTTCCAGGTCTCCCAGCGAGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207204 representing NM_005284
  Red=Cloning site Green=Tags(s)
  
  MNASAASLNDSQVVVVAAEGAAAAATAAGGPDTGEWGPPAAAALGAGGGANGSLELSSQLSAGPPGLLLP
  AVNPWDVLLCVSGTVIAGENALVVALIASTPALRTPMFVLVGSLATADLLAGCGLILHFVFQYLVPSETV
  SLLTVGFLVASFAASVSSLLAITVDRYLSLYNALTYYSRRTLLGVHLLLAATWTVSLGLGLLPVLGWNCL
  AERAACSVVRPLARSHVALLSAAFFMVFGIMLHLYVRICQVVWRHAHQIALQQHCLAPPHLAATRKGVGT
  LAVVLGTFGASWLPFAIYCVVGSHEDPAVYTYATLLPATYNSMINPIIYAFRNQEIQRALWLLLCGCFQS
  KVPFRSRSPSEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005284
• ORF Size: 1086 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005284.1, NM_005284.2, NM_005284.3, NM_005284.4, NP_005275.1
• RefSeq Size: 1667 bp
• RefSeq ORF: 1089 bp
• Locus ID: 2830
• Cytogenetics: 6q21
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 37.7 kDa
• Gene Summary: ''