CACNB1 (NM_000723) Human Tagged ORF Clone

CAT#: RC207230

CACNB1 (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, beta 1 subunit (CACNB1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY424552) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207230 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CACNB1 protein (Cat# TP307230). The protein was produced from HEK293T cells transfected with CACNB1 cDNA clone (Cat# RC207230) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CACNB1
• Synonyms: CAB1; CACNLB1; CCHLB1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207230 representing NM_000723
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCAGAAGACCAGCATGTCCCGGGGCCCTTACCCACCCTCCCAGGAGATCCCCATGGAGGTCTTCG
  ACCCCAGCCCGCAGGGCAAATACAGCAAGAGGAAAGGGCGATTCAAACGGTCAGATGGGAGCACGTCCTC
  GGATACCACATCCAACAGCTTTGTCCGCCAGGGCTCAGCGGAGTCCTACACCAGCCGTCCATCAGACTCT
  GATGTATCTCTGGAGGAGGACCGGGAAGCCTTAAGGAAGGAAGCAGAGCGCCAGGCATTAGCGCAGCTCG
  AGAAGGCCAAGACCAAGCCAGTGGCATTTGCTGTGCGGACAAATGTTGGCTACAATCCGTCTCCAGGGGA
  TGAGGTGCCTGTGCAGGGAGTGGCCATCACCTTCGAGCCCAAAGACTTCCTGCACATCAAGGAGAAATAC
  AATAATGACTGGTGGATCGGGCGGCTGGTGAAGGAGGGCTGTGAGGTTGGCTTCATTCCCAGCCCCGTCA
  AACTGGACAGCCTTCGCCTGCTGCAGGAACAGAAGCTGCGCCAGAACCGCCTCGGCTCCAGCAAATCAGG
  CGATAACTCCAGTTCCAGTCTGGGAGATGTGGTGACTGGCACCCGCCGCCCCACACCCCCTGCCAGTGCC
  AAACAGAAGCAGAAGTCGACAGAGCATGTGCCCCCCTATGACGTGGTGCCTTCCATGAGGCCCATCATCC
  TGGTGGGACCGTCGCTCAAGGGCTACGAGGTTACAGACATGATGCAGAAAGCTTTATTTGACTTCTTGAA
  GCATCGGTTTGATGGCAGGACCTCCATCACTCGTGTGACGGCAGATATTTCCCTGGCTAAGCGCTCAGTT
  CTCAACAACCCCAGCAAACACATCATCATTGAGCGCTCCAACACACGCTCCAGCCTGGCTGAGGTGCAGA
  GTGAAATCGAGCGAATCTTCGAGCTGGCCCGGACCCTTCAGTTGGTCGCTCTGGATGCTGACACCATCAA
  TCACCCAGCCCAGCTGTCCAAGACCTCGCTGGCCCCCATCATTGTTTACATCAAGATCACCTCTCCCAAG
  GTACTTCAAAGGCTCATCAAGTCCCGAGGAAAGTCTCAGTCCAAACACCTCAATGTCCAAATAGCGGCCT
  CGGAAAAGCTGGCACAGTGCCACCCTGAAATGTTTGACATCATCCTGGATGAGAACCAATTGGAGGATGC
  CTGCGAGCATCTGGCGGAGTACTTGGAAGCCTATTGGAAGGCCACACACCCGCCCAGCAGCACGCCACCC
  AATCCGCTGCTGAACCGCACCATGGCTACCGCAGCCCTGGCTGCCAGCCCTGCCCCTGTCTCCAACCTCC
  AGGGACCCTACCTTGCTTCCGGGGACCAGCCACTGGAACGGGCCACCGGGGAGCACGCCAGCATGCACGA
  GTACCCAGGGGAGCTGGGCCAGCCCCCAGGCCTTTACCCCAGCAGCCACCCACCAGGCCGGGCAGGCACG
  CTACGGGCACTGTCCCGCCAAGACACTTTTGATGCCGACACCCCCGGCAGCCGAAACTCTGCCTACACGG
  AGCTGGGAGACTCATGTGTGGACATGGAGACTGACCCCTCAGAGGGGCCAGGGCTTGGAGACCCTGCAGG
  GGGCGGCACGCCCCCAGCCCGACAGGGATCCTGGGAGGACGAGGAAGAAGACTATGAGGAAGAGCTGACC
  GACAACCGGAACCGGGGCCGGAATAAGGCCCGCTACTGCGCTGAGGGTGGGGGTCCAGTTTTGGGGCGCA
  ACAAGAATGAGCTGGAGGGCTGGGGACGAGGCGTCTACATTCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207230 representing NM_000723
  Red=Cloning site Green=Tags(s)
  
  MVQKTSMSRGPYPPSQEIPMEVFDPSPQGKYSKRKGRFKRSDGSTSSDTTSNSFVRQGSAESYTSRPSDS
  DVSLEEDREALRKEAERQALAQLEKAKTKPVAFAVRTNVGYNPSPGDEVPVQGVAITFEPKDFLHIKEKY
  NNDWWIGRLVKEGCEVGFIPSPVKLDSLRLLQEQKLRQNRLGSSKSGDNSSSSLGDVVTGTRRPTPPASA
  KQKQKSTEHVPPYDVVPSMRPIILVGPSLKGYEVTDMMQKALFDFLKHRFDGRTSITRVTADISLAKRSV
  LNNPSKHIIIERSNTRSSLAEVQSEIERIFELARTLQLVALDADTINHPAQLSKTSLAPIIVYIKITSPK
  VLQRLIKSRGKSQSKHLNVQIAASEKLAQCHPEMFDIILDENQLEDACEHLAEYLEAYWKATHPPSSTPP
  NPLLNRTMATAALAASPAPVSNLQGPYLASGDQPLERATGEHASMHEYPGELGQPPGLYPSSHPPGRAGT
  LRALSRQDTFDADTPGSRNSAYTELGDSCVDMETDPSEGPGLGDPAGGGTPPARQGSWEDEEEDYEEELT
  DNRNRGRNKARYCAEGGGPVLGRNKNELEGWGRGVYIR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000723
• ORF Size: 1794 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000723.3, NM_000723.4, NP_000714.3
• RefSeq Size: 3687 bp
• RefSeq ORF: 1797 bp
• Locus ID: 782
• Cytogenetics: 17q12
• Domains: Ca_channel_B, SH3, GuKc
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway
• MW: 65.5 kDa
• Gene Summary: 'The protein encoded by this gene belongs to the calcium channel beta subunit family. It plays an important role in the calcium channel by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Alternative splicing occurs at this locus and three transcript variants encoding three distinct isoforms have been identified. [provided by RefSeq, Jul 2008]'