ZDHHC23 (NM_173570) Human Tagged ORF Clone

CAT#: RC207272

ZDHHC23 (Myc-DDK-tagged)-Human zinc finger, DHHC-type containing 23 (ZDHHC23)

Product Images

Western blot validation of overexpression lysate (Cat# LY406481) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207272 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZDHHC23
• Synonyms: DHHC-23; NIDD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207272 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACACAGAAGGGCAGTATGAAGCCTGTGAAGAAAAAGAAAACCGAAGAACCTGAATTGGAGCCCCTGT
  GCTGCTGCGAGTACATAGATCGGAATGGGGAAAAGAACCACGTGGCTACTTGTTTGTGTGATTGTCAAGA
  TCTGGATGAAGGGTGTGATCGATGGATTACATGTAAATCTTTACAGCCAGAGACTTGTGAAAGAATCATG
  GATACAATTTCTGATCGCCTCCGAATTCCTTGGCTTAGGGGAGCCAAAAAAGTGAACATCAGCATCATCC
  CTCCGCTTGTCCTGCTGCCTGTCTTCCTTCATGTGGCTTCCTGGCATTTCCTCCTGGGGGTGGTGGTTTT
  GACCTCCCTTCCTGTGCTGGCACTGTGGTACTACTACCTCACTAACAGAAGGAAAGAACAGACCCTGTTT
  TTCCTGAGCCTTGGACTGTTCTCTCTGGGCTACATGTACTATGTGTTCCTGCAGGAAGTGGTCCCCAAAG
  GGCGTGTGGGTCCCGTTCAGCTGGCGGTTCTTACCTGCGGGTTATTTCTGATACTCTTAGCCTTGCACAG
  AGCCAAGAAGAATCCAGGCTACCTCAGCAATCCAGCAAGCGGTGACAGATCTCTAAGCAGCAGCCAGCTG
  GAGTGCCTGAGCAGAAAAGGGCAGGAGAAGGCCAAAGGGTTCCCTGGGGCAGACATGTCGGGCAGTCTCA
  ACAATCGCACAACAAAGGATGACCCCAAGGGCTCTTCCAAGATGCCAGCTGGAAGCCCCACCAAAGCGAA
  GGAGGACTGGTGTGCCAAGTGCCAGCTGGTGCGACCAGCCCGGGCATGGCGCTGCCGGATATGTGGCATC
  TGTGTGAGGAGAATGGATCATCATTGTGTCTGGATAAATAGCTGCGTTGGAGAATCAAATCATCAAGCAT
  TTATACTTGCCCTTTTGATCTTCTTGCTCACCTCGGTGTATGGGATCACACTGACCTTGGACACCATTTG
  TAGAGACAGAAGTGTCTTCACAGCTCTTTTCTATTGTCCTGGAGTTTATGCAAATTACAGCTCGGCTCTG
  TCCTTCACCTGCGTGTGGTACTCTGTGATCATCACAGCAGGCATGGCCTACATCTTCCTGATCCAGCTGA
  TCAACATCAGCTACAATGTGACTGAGCGGGAAGTCCAGCAGGCCCTCCGACAGAAGACTGGGCGCCGGCT
  CCTCTGCGGGCTCATCGTGGACACAGGGTTACTTGGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207272 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTQKGSMKPVKKKKTEEPELEPLCCCEYIDRNGEKNHVATCLCDCQDLDEGCDRWITCKSLQPETCERIM
  DTISDRLRIPWLRGAKKVNISIIPPLVLLPVFLHVASWHFLLGVVVLTSLPVLALWYYYLTNRRKEQTLF
  FLSLGLFSLGYMYYVFLQEVVPKGRVGPVQLAVLTCGLFLILLALHRAKKNPGYLSNPASGDRSLSSSQL
  ECLSRKGQEKAKGFPGADMSGSLNNRTTKDDPKGSSKMPAGSPTKAKEDWCAKCQLVRPARAWRCRICGI
  CVRRMDHHCVWINSCVGESNHQAFILALLIFLLTSVYGITLTLDTICRDRSVFTALFYCPGVYANYSSAL
  SFTCVWYSVIITAGMAYIFLIQLINISYNVTEREVQQALRQKTGRRLLCGLIVDTGLLG
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173570
• ORF Size: 1227 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173570.2, NM_173570.3, NM_173570.4, NP_775841.2
• RefSeq Size: 3778 bp
• RefSeq ORF: 1230 bp
• Locus ID: 254887
• Protein Families: Transmembrane
• MW: 45.9 kDa