FSTL5 (NM_020116) Human Tagged ORF Clone

CAT#: RC207327

FSTL5 (Myc-DDK-tagged)-Human follistatin-like 5 (FSTL5), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY412657) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207327 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FSTL5
• Synonyms: DKFZp566D234; KIAA1263
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207327 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTAAGTGCTGGTCAGTTGTCTTGGTTCTCGGATTCATTTTTCTGGAGTCGGAAGGAAGGCCAACCA
  AAGAAGGAGGATATGGCCTTAAATCCTATCAGCCTCTAATGAGATTGCGACATAAGCAGGAAAAAAATCA
  AGAAAGTTCAAGAGTCAAAGGATTTATGATTCAGGATGGCCCTTTTGGATCTTGTGAAAATAAGTACTGT
  GGTTTGGGAAGACACTGTGTTACCAGCAGAGAGACAGGGCAAGCAGAATGTGCCTGTATGGACCTTTGCA
  AACGTCACTACAAACCTGTGTGTGGATCTGACGGAGAATTCTATGAAAACCACTGTGAAGTGCACAGAGC
  TGCTTGCCTGAAAAAACAAAAGATTACCATTGTTCACAATGAAGACTGCTTCTTTAAAGGAGATAAGTGC
  AAGACTACTGAATACAGCAAGATGAAAAATATGCTATTAGATTTACAAAATCAAAAATATATTATGCAAG
  AAAATGAAAATCCTAATGGCGACGACATATCTCGGAAGAAGCTATTGGTGGATCAAATGTTTAAATATTT
  TGATGCAGACAGTAATGGACTTGTAGATATTAATGAACTAACTCAGGTGATAAAACAGGAAGAACTTGGC
  AAGGATCTCTTTGATTGTACTTTGTATGTTCTATTGAAATATGATGATTTTAATGCTGACAAGCACCTGG
  CTCTTGAAGAATTTTATAGAGCATTCCAAGTGATCCAGTTGAGTCTGCCAGAAGATCAGAAACTAAGCAT
  CACTGCAGCAACTGTGGGACAAAGTGCTGTTCTGAGCTGTGCCATTCAAGGAACCCTGAGACCTCCCATT
  ATCTGGAAAAGGAACAATATTATTCTAAATAATTTAGATTTGGAAGACATCAATGACTTTGGAGATGATG
  GGTCCTTGTATATTACTAAGGTTACCACAACTCACGTTGGCAATTACACCTGCTATGCAGATGGCTATGA
  ACAAGTCTATCAGACTCACATCTTCCAAGTGAATGTTCCTCCAGTCATCCGGGTGTATCCAGAGAGTCAG
  GCTAGAGAGCCTGGGGTAACTGCCAGTCTTAGGTGCCATGCAGAGGGCATACCAAAGCCTCAGCTTGGCT
  GGTTGAAGAATGGAATTGATATTACACCAAAGCTTTCCAAACAACTCACGCTTCAAGCAAATGGCAGTGA
  GGTTCACATAAGCAATGTGCGCTATGAAGATACTGGAGCATACACTTGTATCGCAAAGAATGAAGCAGGA
  GTGGATGAAGACATCTCTTCTCTTTTTGTGGAAGACTCTGCTAGAAAGACCCTAGCTAACATATTATGGA
  GAGAAGAAGGTCTGGGAATTGGGAACATGTTCTATGTTTTTTATGAAGATGGAATCAAAGTGATACAACC
  CATAGAATGTGAATTTCAGAGGCACATTAAGCCTAGTGAAAAGCTCCTTGGATTTCAGGATGAAGTCTGT
  CCCAAAGCTGAGGGAGATGAAGTTCAGAGGTGTGTGTGGGCATCAGCTGTTAATGTCAAAGACAAGTTCA
  TTTATGTTGCACAGCCAACTTTGGACAGAGTCCTTATTGTTGATGTGCAGTCCCAAAAAGTTGTTCAGGC
  AGTGAGCACAGACCCTGTCCCAGTTAAATTACACTATGACAAATCACATGATCAGGTCTGGGTGCTAAGC
  TGGGGTACCTTGGAGAAGTCATCACCAACACTACAGGTAATTACCCTGGCCAGTGGGAATGTGCCTCACC
  ACACGATCCACACCCAACCAGTGGGAAAGCAATTTGACAGAGTGGATGATTTTTTCATTCCCACCACAAC
  ACTCATTATCACCCATATGAGGTTTGGATTTATTCTTCATAAAGATGAAGCTGCACTACAAAAAATTGAT
  ATTGAAACCATGTCATACATCAAGACAATTAACTTGAAGGACTATAAGTGCGTTCCTCAGTCATTGGCAT
  ATACACACTTGGGAGGCTACTACTTCATTGGCTGCAAACCTGACAGCACCGGAGCAGTTTCCCCACAGGT
  CATGGTGGACGGTGTAACTGACTCAGTCATTGGGTTCAATAGTGATGTGACGGGCACTCCATATGTCTCT
  CCAGATGGCCACTACCTTGTCAGCATTAATGATGTGAAAGGTCTTGTAAGGGTTCAGTACATTACCATCA
  GAGGAGAAATACAGGAGGCTTTTGATATTTACACAAATCTGCACATATCTGATCTGGCATTTCAACCATC
  CTTTACTGAAGCCCACCAATATAACATCTACGGTAGTTCAAGCACACAAACTGATGTGCTCTTTGTGGAG
  CTCTCTTCTGGGAAGGTCAAGATGATAAAGAGTCTCAAGGAACCACTCAAGGCAGAAGAATGGCCTTGGA
  ACCGGAAAAACAGGCAAATCCAGGACAGTGGCTTGTTTGGTCAATACCTGATGACACCTTCCAAGGACTC
  TCTCTTCATCCTAGATGGACGACTCAATAAATTAAACTGTGAGATCACTGAAGTTGAAAAAGGAAATACA
  GTCATTTGGGTTGGAGATGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207327 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFKCWSVVLVLGFIFLESEGRPTKEGGYGLKSYQPLMRLRHKQEKNQESSRVKGFMIQDGPFGSCENKYC
  GLGRHCVTSRETGQAECACMDLCKRHYKPVCGSDGEFYENHCEVHRAACLKKQKITIVHNEDCFFKGDKC
  KTTEYSKMKNMLLDLQNQKYIMQENENPNGDDISRKKLLVDQMFKYFDADSNGLVDINELTQVIKQEELG
  KDLFDCTLYVLLKYDDFNADKHLALEEFYRAFQVIQLSLPEDQKLSITAATVGQSAVLSCAIQGTLRPPI
  IWKRNNIILNNLDLEDINDFGDDGSLYITKVTTTHVGNYTCYADGYEQVYQTHIFQVNVPPVIRVYPESQ
  AREPGVTASLRCHAEGIPKPQLGWLKNGIDITPKLSKQLTLQANGSEVHISNVRYEDTGAYTCIAKNEAG
  VDEDISSLFVEDSARKTLANILWREEGLGIGNMFYVFYEDGIKVIQPIECEFQRHIKPSEKLLGFQDEVC
  PKAEGDEVQRCVWASAVNVKDKFIYVAQPTLDRVLIVDVQSQKVVQAVSTDPVPVKLHYDKSHDQVWVLS
  WGTLEKSSPTLQVITLASGNVPHHTIHTQPVGKQFDRVDDFFIPTTTLIITHMRFGFILHKDEAALQKID
  IETMSYIKTINLKDYKCVPQSLAYTHLGGYYFIGCKPDSTGAVSPQVMVDGVTDSVIGFNSDVTGTPYVS
  PDGHYLVSINDVKGLVRVQYITIRGEIQEAFDIYTNLHISDLAFQPSFTEAHQYNIYGSSSTQTDVLFVE
  LSSGKVKMIKSLKEPLKAEEWPWNRKNRQIQDSGLFGQYLMTPSKDSLFILDGRLNKLNCEITEVEKGNT
  VIWVGDA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020116
• ORF Size: 2541 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020116.1, NM_020116.2, NM_020116.3, NM_020116.4, NP_064501.1
• RefSeq Size: 4835 bp
• RefSeq ORF: 2544 bp
• Locus ID: 56884
• Protein Families: Secreted Protein
• MW: 95.7 kDa