Metabotropic Glutamate Receptor 3 (GRM3) (NM_000840) Human Tagged ORF Clone

CAT#: RC207379

GRM3 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 3 (GRM3)

Product Images

Western blot validation of overexpression lysate (Cat# LY400297) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207379 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRM3
• Synonyms: GLUR3; GPRC1C; mGlu3; MGLUR3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207379 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATGTTGACAAGACTGCAAGTTCTTACCTTAGCTTTGTTTTCAAAGGGATTTTTACTCTCTTTAG
  GGGACCATAACTTTCTAAGGAGAGAGATTAAAATAGAAGGTGACCTTGTTTTAGGGGGCCTGTTTCCTAT
  TAACGAAAAAGGCACTGGAACTGAAGAATGTGGGCGAATCAATGAAGACCGAGGGATTCAACGCCTGGAA
  GCCATGTTGTTTGCTATTGATGAAATCAACAAAGATGATTACTTGCTACCAGGAGTGAAGTTGGGTGTTC
  ACATTTTTGATACATGTTCAAGGGATACCTATGCATTGGAGCAATCACTGGAGTTTGTCAGGGCATCTTT
  GACAAAAGTGGATGAAGCTGAGTATATGTGTCCTGATGGATCCTATGCCATTCAAGAAAACATCCCACTT
  CTCATTGCAGGGGTCATTGGTGGCTCTTATAGCAGTGTTTCCATACAGGTGGCAAACCTGCTGCGGCTCT
  TCCAGATCCCTCAGATCAGCTACGCATCCACCAGCGCCAAACTCAGTGATAAGTCGCGCTATGATTACTT
  TGCCAGGACCGTGCCCCCCGACTTCTACCAGGCCAAAGCCATGGCTGAGATCTTGCGCTTCTTCAACTGG
  ACCTACGTGTCCACAGTAGCCTCCGAGGGTGATTACGGGGAGACAGGGATCGAGGCCTTCGAGCAGGAAG
  CCCGCCTGCGCAACATCTGCATCGCTACGGCGGAGAAGGTGGGCCGCTCCAACATCCGCAAGTCCTACGA
  CAGCGTGATCCGAGAACTGTTGCAGAAGCCCAACGCGCGCGTCGTGGTCCTCTTCATGCGCAGCGACGAC
  TCGCGGGAGCTCATTGCAGCCGCCAGCCGCGCCAATGCCTCCTTCACCTGGGTGGCCAGCGACGGCTGGG
  GCGCGCAGGAGAGCATCATCAAGGGCAGCGAGCATGTGGCCTACGGCGCCATCACCCTGGAGCTGGCCTC
  CCAGCCTGTCCGCCAGTTCGACCGCTACTTCCAGAGCCTCAACCCCTACAACAACCACCGCAACCCCTGG
  TTCCGGGACTTCTGGGAGCAAAAGTTTCAGTGCAGCCTCCAGAACAAACGCAACCACAGGCGCGTCTGCG
  ACAAGCACCTGGCCATCGACAGCAGCAACTACGAGCAAGAGTCCAAGATCATGTTTGTGGTGAACGCGGT
  GTATGCCATGGCCCACGCTTTGCACAAAATGCAGCGCACCCTCTGTCCCAACACTACCAAGCTTTGTGAT
  GCTATGAAGATCCTGGATGGGAAGAAGTTGTACAAGGATTACTTGCTGAAAATCAACTTCACGGCTCCAT
  TCAACCCAAATAAAGATGCAGATAGCATAGTCAAGTTTGACACTTTTGGAGATGGAATGGGGCGATACAA
  CGTGTTCAATTTCCAAAATGTAGGTGGAAAGTATTCCTACTTGAAAGTTGGTCACTGGGCAGAAACCTTA
  TCGCTAGATGTCAACTCTATCCACTGGTCCCGGAACTCAGTCCCCACTTCCCAGTGCAGCGACCCCTGTG
  CCCCCAATGAAATGAAGAATATGCAACCAGGGGATGTCTGCTGCTGGATTTGCATCCCCTGTGAACCCTA
  CGAATACCTGGCTGATGAGTTTACCTGTATGGATTGTGGGTCTGGACAGTGGCCCACTGCAGACCTAACT
  GGATGCTATGACCTTCCTGAGGACTACATCAGGTGGGAAGACGCCTGGGCCATTGGCCCAGTCACCATTG
  CCTGTCTGGGTTTTATGTGTACATGCATGGTTGTAACTGTTTTTATCAAGCACAACAACACACCCTTGGT
  CAAAGCATCGGGCCGAGAACTCTGCTACATCTTATTGTTTGGGGTTGGCCTGTCATACTGCATGACATTC
  TTCTTCATTGCCAAGCCATCACCAGTCATCTGTGCATTGCGCCGACTCGGGCTGGGGAGTTCCTTCGCTA
  TCTGTTACTCAGCCCTGCTGACCAAGACAAACTGCATTGCCCGCATCTTCGATGGGGTCAAGAATGGCGC
  TCAGAGGCCAAAATTCATCAGCCCCAGTTCTCAGGTTTTCATCTGCCTGGGTCTGATCCTGGTGCAAATT
  GTGATGGTGTCTGTGTGGCTCATCCTGGAGGCCCCAGGCACCAGGAGGTATACCCTTGCAGAGAAGCGGG
  AAACAGTCATCCTAAAATGCAATGTCAAAGATTCCAGCATGTTGATCTCTCTTACCTACGATGTGATCCT
  GGTGATCTTATGCACTGTGTACGCCTTCAAAACGCGGAAGTGCCCAGAAAATTTCAACGAAGCTAAGTTC
  ATAGGTTTTACCATGTACACCACGTGCATCATCTGGTTGGCCTTCCTCCCTATATTTTATGTGACATCAA
  GTGACTACAGAGTGCAGACGACAACCATGTGCATCTCTGTCAGCCTGAGTGGCTTTGTGGTCTTGGGCTG
  TTTGTTTGCACCCAAGGTTCACATCATCCTGTTTCAACCCCAGAAGAATGTTGTCACACACAGACTGCAC
  CTCAACAGGTTCAGTGTCAGTGGAACTGGGACCACATACTCTCAGTCCTCTGCAAGCACGTATGTGCCAA
  CGGTGTGCAATGGGCGGGAAGTCCTCGACTCCACCACCTCATCTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207379 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKMLTRLQVLTLALFSKGFLLSLGDHNFLRREIKIEGDLVLGGLFPINEKGTGTEECGRINEDRGIQRLE
  AMLFAIDEINKDDYLLPGVKLGVHIFDTCSRDTYALEQSLEFVRASLTKVDEAEYMCPDGSYAIQENIPL
  LIAGVIGGSYSSVSIQVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFYQAKAMAEILRFFNW
  TYVSTVASEGDYGETGIEAFEQEARLRNICIATAEKVGRSNIRKSYDSVIRELLQKPNARVVVLFMRSDD
  SRELIAAASRANASFTWVASDGWGAQESIIKGSEHVAYGAITLELASQPVRQFDRYFQSLNPYNNHRNPW
  FRDFWEQKFQCSLQNKRNHRRVCDKHLAIDSSNYEQESKIMFVVNAVYAMAHALHKMQRTLCPNTTKLCD
  AMKILDGKKLYKDYLLKINFTAPFNPNKDADSIVKFDTFGDGMGRYNVFNFQNVGGKYSYLKVGHWAETL
  SLDVNSIHWSRNSVPTSQCSDPCAPNEMKNMQPGDVCCWICIPCEPYEYLADEFTCMDCGSGQWPTADLT
  GCYDLPEDYIRWEDAWAIGPVTIACLGFMCTCMVVTVFIKHNNTPLVKASGRELCYILLFGVGLSYCMTF
  FFIAKPSPVICALRRLGLGSSFAICYSALLTKTNCIARIFDGVKNGAQRPKFISPSSQVFICLGLILVQI
  VMVSVWLILEAPGTRRYTLAEKRETVILKCNVKDSSMLISLTYDVILVILCTVYAFKTRKCPENFNEAKF
  IGFTMYTTCIIWLAFLPIFYVTSSDYRVQTTTMCISVSLSGFVVLGCLFAPKVHIILFQPQKNVVTHRLH
  LNRFSVSGTGTTYSQSSASTYVPTVCNGREVLDSTTSSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000840
• ORF Size: 2637 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000840.2, NP_000831.2
• RefSeq Size: 4260 bp
• RefSeq ORF: 2640 bp
• Locus ID: 2913
• Cytogenetics: 7q21.11-q21.12
• Domains: 7tm_3, ANF_receptor
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 98.9 kDa
• Gene Summary: 'L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. [provided by RefSeq, Jul 2008]'