EXTL2 (NM_001439) Human Tagged ORF Clone

CAT#: RC207406

EXTL2 (Myc-DDK-tagged)-Human exostoses (multiple)-like 2 (EXTL2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY419935) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207406 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified EXTL2 protein (Cat# TP307406). The protein was produced from HEK293T cells transfected with EXTL2 cDNA clone (Cat# RC207406) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EXTL2
• Synonyms: EXTR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207406 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTGTTGCCACATCTGCAAACTTCCTGGGAGAGTAATGGGGATTCGAGTGCTTCGATTATCTTTGG
  TGGTCATCCTCGTATTATTACTGGTAGCTGGTGCTTTGACTGCCTTACTTCCCAGTGTTAAAGAAGACAA
  GATGCTCATGTTGCGTAGGGAAATAAAATCCCAGGGCAAGTCCACCATGGACTCCTTTACTCTCATAATG
  CAGACGTACAACAGAACAGATCTCTTATTGAAACTTTTAAATCATTATCAGGCTGTACCAAATCTGCACA
  AAGTGATTGTGGTATGGAACAATATTGGAGAGAAGGCACCAGATGAATTATGGAATTCTCTAGGGCCCCA
  CCCTATCCCTGTGATCTTCAAACAACAGACAGCAAACAGGATGAGAAATCGACTCCAGGTCTTTCCTGAA
  CTGGAAACCAATGCAGTGTTGATGGTAGATGATGACACACTCATCAGCACCACAGACCTTGTTTTTGCTT
  TCTCAGTTTGGCAGCAATTTCCTGATCAAATTGTAGGATTTGTTCCTAGAAAGCACGTCTCTACTTCATC
  AGGTATCTACAGTTATGGAAGTTTTGAAATGCAAGCACCAGGGTCTGGAAATGGTGACCAGTACTCTATG
  GTGCTGATTGGAGCCTCATTCTTCAATAGCAAATATCTTGAATTATTTCAGAGGCAACCTGCAGCTGTCC
  ATGCTTTGATAGATGATACTCAAAACTGTGATGATATTGCCATGAATTTTATCATTGCCAAGCATATTGG
  CAAGACTTCAGGGATATTTGTGAAGCCTGTAAACATGGACAATTTGGAAAAAGAAACCAACAGTGGCTAT
  TCTGGAATGTGGCATCGAGCTGAGCACGCTCTGCAGAGGTCTTATTGTATAAATAAGCTTGTTAATATCT
  ATGATAGCATGCCCTTAAGATACTCCAACATTATGATTTCCCAGTTTGGTTTTCCATATGCCAACTACAA
  AAGAAAAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207406 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRCCHICKLPGRVMGIRVLRLSLVVILVLLLVAGALTALLPSVKEDKMLMLRREIKSQGKSTMDSFTLIM
  QTYNRTDLLLKLLNHYQAVPNLHKVIVVWNNIGEKAPDELWNSLGPHPIPVIFKQQTANRMRNRLQVFPE
  LETNAVLMVDDDTLISTTDLVFAFSVWQQFPDQIVGFVPRKHVSTSSGIYSYGSFEMQAPGSGNGDQYSM
  VLIGASFFNSKYLELFQRQPAAVHALIDDTQNCDDIAMNFIIAKHIGKTSGIFVKPVNMDNLEKETNSGY
  SGMWHRAEHALQRSYCINKLVNIYDSMPLRYSNIMISQFGFPYANYKRKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001439
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001439.1, NM_001439.2, NM_001439.3, NP_001430.1
• RefSeq Size: 3217 bp
• RefSeq ORF: 993 bp
• Locus ID: 2135
• Cytogenetics: 1p21.2
• Protein Families: Transmembrane
• Protein Pathways: Heparan sulfate biosynthesis
• MW: 37.5 kDa
• Gene Summary: ''