TAF7 (NM_005642) Human Tagged ORF Clone

CAT#: RC207437

TAF7 (Myc-DDK-tagged)-Human TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa (TAF7)

Product Images

Western blot validation of overexpression lysate (Cat# LY417173) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207437 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TAF7 protein (Cat# TP307437). The protein was produced from HEK293T cells transfected with TAF7 cDNA clone (Cat# RC207437) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAF7
• Synonyms: TAF2F; TAFII55
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207437 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAAAGCAAAGATGATGCTCCTCACGAACTGGAGAGCCAGTTTATCTTACGTCTGCCTCCAGAAT
  ATGCCTCTACTGTGAGAAGGGCAGTACAGTCTGGTCATGTCAACCTCAAGGACAGACTGACAATTGAGTT
  ACATCCTGATGGGCGTCATGGAATCGTCAGAGTGGACCGTGTTCCATTGGCCTCAAAATTAGTAGACCTG
  CCCTGTGTTATGGAAAGCTTGAAAACCATTGATAAAAAAACTTTTTACAAGACAGCTGATATCTGTCAGA
  TGCTTGTATCCACAGTTGATGGTGATCTCTATCCTCCTGTGGAGGAGCCAGTTGCTAGCACTGATCCTAA
  AGCAAGCAAGAAAAAGGATAAGGACAAAGAGAAAAAGTTTATCTGGAACCACGGAATTACTCTGCCTCTA
  AAGAATGTCAGGAAGAGAAGGTTCCGGAAGACAGCAAAGAAGAAATATATTGAATCTCCAGATGTTGAAA
  AAGAAGTGAAACGATTGCTGAGTACAGATGCTGAAGCTGTTAGTACTCGGTGGGAAATAATTGCCGAAGA
  TGAAACAAAGGAGGCAGAAAATCAAGGCCTGGATATCTCTTCTCCAGGAATGTCTGGTCACAGGCAGGGC
  CATGACTCATTAGAACATGATGAGCTTCGGGAGATATTCAATGACCTCAGCAGCAGCAGTGAGGATGAAG
  ATGAGACCCAGCATCAAGATGAAGAAGATATAAACATCATTGACACGGAGGAAGATCTGGAGAGACAGCT
  ACAGGACAAGCTAAATGAATCAGATGAACAGCACCAGGAAAATGAAGGAACCAATCAGCTGGTTATGGGA
  ATTCAGAAGCAGATTGACAACATGAAAGGCAAGCTCCAAGAGACCCAGGACAGGGCAAAACGACAAGAGG
  ATCTCATCATGAAAGTGGAAAATCTGGCTCTCAAGAACAGATTTCAGGCTGTACTGGATGAGCTCAAACA
  AAAGGAAGACCGAGAAAAGGAGCAACTCAGCTCTTTGCAAGAGGAGCTAGAATCACTCCTAGAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207437 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSKSKDDAPHELESQFILRLPPEYASTVRRAVQSGHVNLKDRLTIELHPDGRHGIVRVDRVPLASKLVDL
  PCVMESLKTIDKKTFYKTADICQMLVSTVDGDLYPPVEEPVASTDPKASKKKDKDKEKKFIWNHGITLPL
  KNVRKRRFRKTAKKKYIESPDVEKEVKRLLSTDAEAVSTRWEIIAEDETKEAENQGLDISSPGMSGHRQG
  HDSLEHDELREIFNDLSSSSEDEDETQHQDEEDINIIDTEEDLERQLQDKLNESDEQHQENEGTNQLVMG
  IQKQIDNMKGKLQETQDRAKRQEDLIMKVENLALKNRFQAVLDELKQKEDREKEQLSSLQEELESLLEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005642
• ORF Size: 1047 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005642.1, NM_005642.2, NP_005633.2
• RefSeq Size: 2310 bp
• RefSeq ORF: 1050 bp
• Locus ID: 6879
• Cytogenetics: 5q31.3
• Domains: TAFII55_N
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• MW: 40.3 kDa
• Gene Summary: 'The intronless gene for this transcription coactivator is located between the protocadherin beta and gamma gene clusters on chromosome 5. The protein encoded by this gene is a component of the TFIID protein complex, a complex which binds to the TATA box in class II promoters and recruits RNA polymerase II and other factors. This particular subunit interacts with the largest TFIID subunit, as well as multiple transcription activators. The protein is required for transcription by promoters targeted by RNA polymerase II. [provided by RefSeq, Jul 2008]'