ZFP30 (NM_014898) Human Tagged ORF Clone

CAT#: RC207525

ZFP30 (Myc-DDK-tagged)-Human zinc finger protein 30 homolog (mouse) (ZFP30)

Product Images

Western blot validation of overexpression lysate (Cat# LY414949) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207525 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZFP30
• Synonyms: ZNF745
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207525 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCGTGATTTGGTGATGTTCAGAGATGTGGCTGTAGACTTTTCTCAGGAAGAATGGGAATGCCTGA
  ACTCATATCAGAGGAATTTGTACAGAGATGTGATATTAGAGAACTATAGCAACTTGGTGTCACTGGCAGG
  ATGTTCTATTTCTAAGCCAGATGTGATCACCCTATTGGAACAAGGGAAAGAGCCCTGGATGGTTGTGAGG
  GATGAGAAAAGAAGATGGACTCTAGATTTGGAATCCAGATATGACACTAAAAAGTTATTTCAAGGAAAGG
  ATATTTATGAAATGAACTTATCTCAGTGGAAGGTAATGGAAAGAATTAAAAGCTGTGGACTTGAAGAACA
  AGAAAGTCCTCATGAGGTGTGTTTCAGGCAAGTGACAAAAACCACCTCTGAAAAAATGCCTACTTACAGA
  AAACTCACATCTCTTCCTCTGTATCAGAAAAGTCATAACAGAGAGAAACCCTACGAATGTGGGGAATGTG
  GGAAGGCTTTTAGAGTACGACAACAACTTACTTTCCATCAAAGAATTCATACTGGTGAGAAACCCTATGA
  ATGTAAAGAATGTGGAAAAGCCTTTAGACAGTGTGCCCACCTCAGTCGACATCAGAGAATTCATACTTCT
  GACAAACTCTATGAATGTAAAAAATGTGGAAAGATCTTCACATGTGGCTCAGACCTTCGAGTACATCAGA
  GAATTCATATTGGTGAGAAGCCGTATGAATGTAAAGAATGTGGGAAAGCCTTTAGAGTTAGAGGACAACT
  TAATCTCCATCAAAGGATTCACACGGGTGAGAAACCCTATGAATGTAAAGAATGTGGGAAGGCCTTTAGG
  CAGTATGCACACCTTACTCGACATCAGAGACTGAACATTGCTGAGAAGTGCTATGAGTGTAAGGAATGTG
  GTCAGGCCTTTCTGTGTAGTACAGGCCTTCGACTACATCACAAACTTCATACTGGAGAAAAACCCTATGA
  ATGTAAGGAGTGTGGAAAGGCCTTTAGAGTGCGGCAGCAACTTACTCTCCATCAGAGAATTCACACTGGT
  GAGAAGCCTTATGATTGTAAGGAATGTGGGAAGACTTTCAGTCGTGGCTATCATCTAACTCTCCATCAGA
  GAATACATACTGGTGAAAAGCCCTATGAATGTAAGGAATGTCAGAAGTTCTTTCGTCGTTACTCAGAACT
  TATTTCACATCAGGGTATTCACATTGGAGAGAAACCTTATGAATGTAAGGAATGTGGGAAGGCATTTAGG
  CTGTTCTCACAGCTTACTCAACATCAGAGTATTCATTTTGGTGAGAAACCCTTTAAGTGTAAGGAATGTG
  AGAAGACTTTTAGACTGCTTTCACAACTTACCCAACATCAAAGTATTCATACTGGTGAAAAGCCCTATGA
  CTGTAAGGAATGTGGAAAGGCCTTTAGACTTCATTCATCACTGATTCAACATCAGAGAATTCATTCTGGT
  GAGAAACCATACAAATGTAAGGAATGTAAAAAGGCATTTAGACAACATTCACATCTTACTTACCATCAGC
  GAATTCATAATGTAACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207525 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MARDLVMFRDVAVDFSQEEWECLNSYQRNLYRDVILENYSNLVSLAGCSISKPDVITLLEQGKEPWMVVR
  DEKRRWTLDLESRYDTKKLFQGKDIYEMNLSQWKVMERIKSCGLEEQESPHEVCFRQVTKTTSEKMPTYR
  KLTSLPLYQKSHNREKPYECGECGKAFRVRQQLTFHQRIHTGEKPYECKECGKAFRQCAHLSRHQRIHTS
  DKLYECKKCGKIFTCGSDLRVHQRIHIGEKPYECKECGKAFRVRGQLNLHQRIHTGEKPYECKECGKAFR
  QYAHLTRHQRLNIAEKCYECKECGQAFLCSTGLRLHHKLHTGEKPYECKECGKAFRVRQQLTLHQRIHTG
  EKPYDCKECGKTFSRGYHLTLHQRIHTGEKPYECKECQKFFRRYSELISHQGIHIGEKPYECKECGKAFR
  LFSQLTQHQSIHFGEKPFKCKECEKTFRLLSQLTQHQSIHTGEKPYDCKECGKAFRLHSSLIQHQRIHSG
  EKPYKCKECKKAFRQHSHLTYHQRIHNVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014898
• ORF Size: 1557 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014898.1, NM_014898.2, NP_055713.1
• RefSeq Size: 4611 bp
• RefSeq ORF: 1560 bp
• Locus ID: 22835
• Domains: KRAB, zf-C2H2
• Protein Families: Transcription Factors
• MW: 61.6 kDa
• Gene Summary: May be involved in transcriptional regulation. [UniProtKB/Swiss-Prot Function]