Host cell factor C2 (HCFC2) (NM_013320) Human Tagged ORF Clone
CAT#: RC207543
HCFC2 (Myc-DDK-tagged)-Human host cell factor C2 (HCFC2)
"NM_013320" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | HCFC2 |
Synonyms | HCF-2; HCF2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207543 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTCCCAGCCTCCTCAACTGGAGGCGAGTTTCTTCCTTCACGGGGCCGGTCCCCCGCGCCCGGC ACGGACACCGAGCGGTGGCCATCCGGGAGCTGATGATCATCTTTGGAGGGGGAAATGAGGGCATCGCGGA TGAGCTGCACGTCTACAACACGGCTACGAATCAGTGGTTTCTGCCAGCTGTTAGAGGAGATATCCCTCCA GGCTGTGCTGCCCATGGATTTGTCTGTGATGGTACCAGAATATTAGTATTTGGGGGAATGGTTGAATATG GAAGATACAGCAATGAGTTATATGAGTTACAAGCAAGTCGTTGGTTATGGAAAAAAGTGAAACCCCATCC CCCTCCTTCTGGTTTACCTCCTTGTCCTCGGCTTGGACATAGCTTCTCTTTATATGGTAACAAATGCTAT TTGTTTGGTGGCCTGGCAAACGAAAGCGAAGATTCAAACAATAATGTTCCCAGATATTTAAATGATTTTT ATGAGTTGGAGCTACAGCATGGCTCTGGTGTTGTGGGTTGGAGCATTCCAGTGACTAAAGGGGTTGTGCC TTCTCCAAGAGAATCCCACACAGCTGTTATATATTGCAAAAAAGATTCTGGAAGTCCTAAAATGTATGTT TTTGGTGGAATGTGTGGTGCTCGCCTGGATGACCTATGGCAGCTTGACTTAGAAACTATGTCATGGTCAA AACCAGAAACTAAAGGGACAGTGCCACTTCCACGAAGCCTTCATACAGCCAGTGTTATAGGAAACAAGAT GTACATTTTTGGTGGATGGGTCCCACATAAGGGGGAAAATACTGAGACTTCACCTCATGATTGTGAATGG AGATGTACCAGTTCATTTTCTTACCTAAATCTGGATACAACAGAGTGGACCACCCTAGTATCAGATTCTC AGGAAGATAAAAAAAATTCAAGACCAAGACCAAGAGCTGGCCACTGTGCTGTTGCAATCGGCACTCGATT GTATTTTTGGAGTGGAAGAGATGGCTACAAAAAAGCACTGAATAGTCAAGTTTGCTGCAAGGATCTTTGG TATCTTGATACTGAGAAACCACCGGCACCATCTCAAGTACAGCTGATCAAAGCCACTACCAACTCCTTTC ATGTCAAGTGGGATGAAGTGTCTACAGTTGAGGGCTATCTTTTGCAGTTGAGTACAGACTTGCCATACCA AGCTGCATCATCAGATTCTTCAGCAGCACCAAATATGCAAGGAGTCAGGATGGACCCTCACAGACAAGGC AGTAATAACATCGTTCCTAACAGTATCAATGATACAATAAACAGCACAAAAACTGAACAGCCAGCCACAA AAGAAACTTCAATGAAAAACAAACCAGACTTTAAAGCACTGACGGATTCTAATGCCATTTTATATCCATC TTTGGCATCAAATGCTTCTAATCATAATAGTCATGTGGTGGATATGCTAAGGAAAAATGAAGGTCCTCAC ACTTCAGCAAATGTAGGTGTTCTAAGTAGTTGCCTGGATGTAAGAACAGTAATTCCTGAAACATCTGTAT CCAGTACTGTTTCCAGCACACAAACTATGGTAACCCAGCAGACCATTAAAACTGAATCATCCAGTACAAA TGGGGCAGTTGTTAAAGATGAAACTTCACTAACAACATTCAGTACCAAATCTGAAGTTGATGAAACATAT GCACTGCCTGCAACGAAGATCAGCCGTGTAGAGACACATGCTACAGCAACGCCGTTTTCTAAAGAGACTC CTTCAAATCCAGTGGCCACAGTGAAAGCGGGAGAACGACAATGGTGTGATGTGGGAATTTTTAAAAATAA TACAGCTTTGGTGAGCCAGTTTTATTTGCTGCCAAAAGGGAAGCAAAGCATCTCAAAGGTAGGAAATGCA GATGTACCTGACTACAGCTTGCTTAAGAAACAAGATCTTGTTCCAGGCACAGGATACAGATTCAGGGTTG CTGCAATCAATGGTTGTGGGATAGGTCCTTTCAGCAAAATCAGTGAATTTAAAACTTGTATTCCTGGTTT TCCTGGAGCTCCTTCTGCAGTCAGAATTTCAAAGAATGTTGAAGGTATCCACCTTTCCTGGGAACCTCCA ACCTCACCTTCTGGAAATATTTTGGAATATTCAGCCTACTTGGCTATCCGCACAGCACAGATACAAGATA ATCCAAGTCAACTTGTGTTCATGAGGATTTATTGTGGTCTTAAGACATCATGTATAGTAACTGCTGGGCA ACTTGCAAATGCACATATTGATTATACATCCAGGCCTGCCATTGTGTTCAGGATATCAGCAAAGAATGAA AAGGGATATGGACCAGCTACACAAGTTCGGTGGCTTCAAGGTAACAATAAGAAAGCACCTTTAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207543 protein sequence
Red=Cloning site Green=Tags(s) MAAPSLLNWRRVSSFTGPVPRARHGHRAVAIRELMIIFGGGNEGIADELHVYNTATNQWFLPAVRGDIPP GCAAHGFVCDGTRILVFGGMVEYGRYSNELYELQASRWLWKKVKPHPPPSGLPPCPRLGHSFSLYGNKCY LFGGLANESEDSNNNVPRYLNDFYELELQHGSGVVGWSIPVTKGVVPSPRESHTAVIYCKKDSGSPKMYV FGGMCGARLDDLWQLDLETMSWSKPETKGTVPLPRSLHTASVIGNKMYIFGGWVPHKGENTETSPHDCEW RCTSSFSYLNLDTTEWTTLVSDSQEDKKNSRPRPRAGHCAVAIGTRLYFWSGRDGYKKALNSQVCCKDLW YLDTEKPPAPSQVQLIKATTNSFHVKWDEVSTVEGYLLQLSTDLPYQAASSDSSAAPNMQGVRMDPHRQG SNNIVPNSINDTINSTKTEQPATKETSMKNKPDFKALTDSNAILYPSLASNASNHNSHVVDMLRKNEGPH TSANVGVLSSCLDVRTVIPETSVSSTVSSTQTMVTQQTIKTESSSTNGAVVKDETSLTTFSTKSEVDETY ALPATKISRVETHATATPFSKETPSNPVATVKAGERQWCDVGIFKNNTALVSQFYLLPKGKQSISKVGNA DVPDYSLLKKQDLVPGTGYRFRVAAINGCGIGPFSKISEFKTCIPGFPGAPSAVRISKNVEGIHLSWEPP TSPSGNILEYSAYLAIRTAQIQDNPSQLVFMRIYCGLKTSCIVTAGQLANAHIDYTSRPAIVFRISAKNE KGYGPATQVRWLQGNNKKAPLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013320 |
ORF Size | 2376 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013320.1, NM_013320.2, NP_037452.1 |
RefSeq Size | 5743 bp |
RefSeq ORF | 2379 bp |
Locus ID | 29915 |
Domains | FN3, Kelch |
Protein Families | Transcription Factors |
MW | 86.8 kDa |
Gene Summary | This gene encodes one of two proteins which interact with VP16, a herpes simplex virus protein that initiates virus infection. Both the encoded protein and the original Herpes host cell factor interact with VP16 through a beta-propeller domain. The original Herpes host cell factor, however, is effective at initiating viral infection while the encoded protein is not. Transcripts of varying length due to alternative polyadenylation signals have been described. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC108617 | HCFC2 (untagged)-Human host cell factor C2 (HCFC2) |
USD 310.00 |
|
RG207543 | HCFC2 (GFP-tagged) - Human host cell factor C2 (HCFC2) |
USD 970.00 |
|
RC207543L3 | Lenti ORF clone of Human host cell factor C2 (HCFC2), Myc-DDK-tagged |
USD 1,080.00 |
|
RC207543L4 | Lenti ORF clone of Human host cell factor C2 (HCFC2), mGFP tagged |
USD 1,080.00 |
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