HPS5 (NM_181508) Human Tagged ORF Clone

CAT#: RC207552

HPS5 (Myc-DDK-tagged)-Human Hermansky-Pudlak syndrome 5 (HPS5), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY402113) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211614 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HPS5
• Synonyms: AIBP63; BLOC2S2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207552 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATGTGTCTTCAGAACACAAAGGCCGAAGAGTCACAGCTCTCTGCTGGGATACAGCTATTCTTAGAG
  TTTTTGTAGGTGATCATGCTGGGAAGGTTTCTGCTATCAAACTCAATACTTCTAAACAAGCAAAGGCAGC
  TGCTGCTTTTGTGATGTTTCCTGTTCAGACAATCACAACTGTTGACTCCTGTGTTGTACAGTTAGATTAT
  TTGGATGGAAGGCTACTTATATCTTCACTTACTCGATCCTTCTTGTGTGACACTGAGAGAGAAAAGTTTT
  GGAAAATTGGAAACAAGGAAAGAGATGGAGAATATGGAGCTTGTTTCTTTCCTGGAAGATGTTCTGGGGG
  CCAGCAACCTCTGATATATTGTGCTCGCCCAGGCTCTAGGATGTGGGAAGTGAACTTTGATGGAGAAGTT
  ATAAGTACACATCAGTTCAAGAAACTCCTCTCGTTGCCACCTCTCCCTGTGATTACTCTAAGATCAGAAC
  CTCAGTATGATCATACAGCTGGATCCTCCCAGTCTTTGTCTTTCCCCAAACTCTTACATCTTAGTGAGCA
  TTGTGTGCTGACTTGGACAGAAAGAGGAATTTATATTTTCATTCCTCAGAATGTTCAAGTTCTTCTTTGG
  AGTGAAGTCAAAGATATTCAGGATGTGGCTGTCTGTAGGAATGAATTGTTCTGTTTGCACCTAAATGGGA
  AAGTCTCACATCTCTCCCTGATATCTGTGGAGCGCTGTGTGGAACGCCTGCTAAGAAGAGGCCTATGGAA
  CTTGGCTGCTCGTACATGCTGTCTTTTCCAAAATTCTGTCATTGCCAGCAGAGCAAGAAAAACTTTGACT
  GCAGATAAATTGGAGCATTTGAAATCTCAGCTGGACCATGGCACCTACAATGATCTAATTTCTCAACTGG
  AAGAATTGATCTTAAAATTTGAACCTTTGGATTCAGCTTGTAGCAGTAGAAGAAGCTCCATTTCATCACA
  TGAAAGTTTCAGCATCTTGGACTCTGGTATTTATCGTATCATTAGTAGTAGAAGAGGCAGTCAGTCAGAT
  GAAGACTCTTGCTCCCTTCACAGCCAAACCCTCTCAGAAGATGAGAGATTTAAAGAATTCACCTCACAGC
  AGGAAGAGGACCTGCCAGATCAGTGTTGTGGCTCACACGGAAATGAAGACAATGTTTCTCATGCTCCAGT
  GATGTTTGAGACAGATAAGAATGAAACTTTTCTCCCGTTCGGCATTCCATTACCATTTCGTTCTCCATCT
  CCTCTTGTGTCTCTTCAGGCTGTCAAAGAAAGTGTTTCTAGCTTTGTGCGTAAAACTACTGAGAAGATTG
  GCACCCTTCACACGAGCCCTGATCTGAAAGTGAGACCAGAGCTCAGGGGTGATGAGCAATCATGTGAAGA
  GGATGTGAGTTCAGATACCTGCCCAAAGGAGGAAGACACTGAGGAGGAAAAAGAGGTAACTAGTCCACCT
  CCAGAAGAAGACAGGTTCCAGGAGCTTAAAGTAGCAACAGCAGAAGCAATGACCAAGCTACAGGACCCTC
  TGGTTTTATTTGAATCCGAGTCTCTGAGAATGGTTTTACAGGAGTGGCTTTCACATTTAGAAAAAACATT
  TGCCATGAAGGACTTTTCAGGTGTTTCAGATACTGACAACTCATCCATGAAATTGAACCAGGATGTGCTA
  TTAGTTAATGAATCAAAAAAGGGAATATTAGATGAAGATAATGAAAAAGAAAAAAGGGACTCTTTAGGCA
  ATGAAGAATCTGTTGATAAAACAGCATGTGAATGTGTAAGGAGTCCAAGGGAGTCTTTGGATGACCTGTT
  TCAAATATGTTCTCCATGCGCCATTGCAAGTGGTCTTCGGAACGACCTGGCTGAATTGACAACATTATGT
  TTGGAGTTGAATGTATTGAATTCTAAGATCAAAAGCACCAGTGGACATGTGGACCACACTTTGCAACAGT
  ACTCTCCTGAAATTCTGGCTTGCCAGTTCCTGAAGAAGTACTTTTTTCTCCTGAACTTGAAAAGAGCGAA
  GGAGAGTATCAAGCTTAGTTACAGTAATAGCCCTTCTGTTTGGGATACTTTTATTGAAGGATTGAAAGAA
  ATGGCAAGTTCCAATCCTGTGTATATGGAGATGGAAAAAGGAGATCTACCAACAAGGTTAAAGTTACTAG
  ATGACGAGGTTCCTTTTGATAGTCCGTTGTTGGTTGTTTATGCTACCCGGTTGTATGAAAAGTTTGGGGA
  GTCTGCTCTTCGATCCTTAATCAAGTTCTTTCCATCCATTTTGCCATCGGATATCATACAACTTTGTCAT
  CATCATCCTGCTGAGTTTTTGGCCTATTTAGACAGTCTGGTGAAATCAAGGCCTGAAGATCAGCGGTCAT
  CTTTTCTTGAGTCCCTTCTGCAACCAGAGTCTTTAAGGTTGGATTGGCTGCTTTTGGCAGTGTCCCTTGA
  TGCTCCACCAAGCACCAGCACAATGGATGATGAAGGTTATCCCAGGCCTCATTCACACTTGCTTTCCTGG
  GGTTACAGTCAGCTGATCCTTCATCTAATTAAACTTCCTGCAGATTTTATAACCAAAGAGAAAATGACAG
  ACATCTGCAGGTCTTGTGGTTTCTGGCCTGGATATCTAATTCTCTGTTTGGAGCTGGAGAGAAGAAGAGA
  GGCCTTCACCAATATTGTGTATCTGAATGATATGAGCCTGATGAAAGGGGACAATGGTTGGATCCCAGAG
  ACCGTGGAGGAATGGAAGCTTCTCCTTCATCTCATACAGAGCAAGAGCACGAGGCCAGCCCCCCAGGAGT
  CACTAAATGGGAGCCTCAGTGATGGGCCTTCCCCCATCAATGTGGAGAATGTGGCACTTCTGTTAGCTAA
  GGCCATGGGCCCAGATCGGGCTTGGTCACTGCTACAGGAATGTGGTCTGGCCCTTGAGTTGTCAGAGAAG
  TTTACCAGAACCTGCGATATCCTGAGGATTGCTGAGAAAAGGCAGAGGGCCTTGATACAAAGCATGCTTG
  AAAAATGCGATCGGTTTCTCTGGTCCCAGCAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207552 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYVSSEHKGRRVTALCWDTAILRVFVGDHAGKVSAIKLNTSKQAKAAAAFVMFPVQTITTVDSCVVQLDY
  LDGRLLISSLTRSFLCDTEREKFWKIGNKERDGEYGACFFPGRCSGGQQPLIYCARPGSRMWEVNFDGEV
  ISTHQFKKLLSLPPLPVITLRSEPQYDHTAGSSQSLSFPKLLHLSEHCVLTWTERGIYIFIPQNVQVLLW
  SEVKDIQDVAVCRNELFCLHLNGKVSHLSLISVERCVERLLRRGLWNLAARTCCLFQNSVIASRARKTLT
  ADKLEHLKSQLDHGTYNDLISQLEELILKFEPLDSACSSRRSSISSHESFSILDSGIYRIISSRRGSQSD
  EDSCSLHSQTLSEDERFKEFTSQQEEDLPDQCCGSHGNEDNVSHAPVMFETDKNETFLPFGIPLPFRSPS
  PLVSLQAVKESVSSFVRKTTEKIGTLHTSPDLKVRPELRGDEQSCEEDVSSDTCPKEEDTEEEKEVTSPP
  PEEDRFQELKVATAEAMTKLQDPLVLFESESLRMVLQEWLSHLEKTFAMKDFSGVSDTDNSSMKLNQDVL
  LVNESKKGILDEDNEKEKRDSLGNEESVDKTACECVRSPRESLDDLFQICSPCAIASGLRNDLAELTTLC
  LELNVLNSKIKSTSGHVDHTLQQYSPEILACQFLKKYFFLLNLKRAKESIKLSYSNSPSVWDTFIEGLKE
  MASSNPVYMEMEKGDLPTRLKLLDDEVPFDSPLLVVYATRLYEKFGESALRSLIKFFPSILPSDIIQLCH
  HHPAEFLAYLDSLVKSRPEDQRSSFLESLLQPESLRLDWLLLAVSLDAPPSTSTMDDEGYPRPHSHLLSW
  GYSQLILHLIKLPADFITKEKMTDICRSCGFWPGYLILCLELERRREAFTNIVYLNDMSLMKGDNGWIPE
  TVEEWKLLLHLIQSKSTRPAPQESLNGSLSDGPSPINVENVALLLAKAMGPDRAWSLLQECGLALELSEK
  FTRTCDILRIAEKRQRALIQSMLEKCDRFLWSQQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181508
• ORF Size: 3045 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181508.1, NP_852609.1
• RefSeq Size: 4536 bp
• RefSeq ORF: 3048 bp
• Locus ID: 11234
• MW: 114.8 kDa
• Gene Summary: This gene encodes a protein that may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. This protein interacts with Hermansky-Pudlak syndrome 6 protein and may interact with the cytoplasmic domain of integrin, alpha-3. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 5. Multiple transcript variants encoding two distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]