GPR65 (NM_003608) Human Tagged ORF Clone

CAT#: RC207600

GPR65 (Myc-DDK-tagged)-Human G protein-coupled receptor 65 (GPR65)

Product Images

Western blot validation of overexpression lysate (Cat# LY401195) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207600 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPR65
• Synonyms: hTDAG8; TDAG8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207600 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGCACATGTATTGAAGAACAGCATGACCTGGATCACTATTTGTTTCCCATTGTTTACATCTTTG
  TGATTATAGTCAGCATTCCAGCCAATATTGGATCTCTGTGTGTGTCTTTCCTGCAAGCAAAGAAGGAAAG
  TGAACTAGGAATTTACCTCTTCAGTTTGTCACTATCAGATTTACTCTATGCATTAACTCTCCCTTTATGG
  ATTGATTATACTTGGAATAAAGACAACTGGACTTTCTCTCCTGCCTTGTGCAAAGGGAGTGCTTTTCTCA
  TGTACATGAATTTTTACAGCAGCACAGCATTCCTCACCTGCATTGCCGTTGATCGGTATTTGGCTGTTGT
  CTACCCTTTGAAGTTTTTTTTCCTAAGGACAAGAAGATTTGCACTCATGGTCAGCCTGTCCATCTGGATA
  TTGGAAACCATCTTCAATGCTGTCATGTTGTGGGAAGATGAAACAGTTGTTGAATATTGCGATGCCGAAA
  AGTCTAATTTTACTTTATGCTATGACAAATACCCTTTAGAGAAATGGCAAATCAACCTCAACTTGTTCAG
  GACGTGTACAGGCTATGCAATACCTTTGGTCACCATCCTGATCTGCAACCGGAAAGTCTACCAAGCTGTG
  CGGCACAATAAAGCCACGGAAAACAAGGAAAAGAAGAGAATCATAAAACTACTTGTCAGCATCACAGTTA
  CTTTTGTCTTATGCTTTACTCCCTTTCATGTGATGTTGCTGATTCGCTGCATTTTAGAGCATGCTGTGAA
  CTTCGAAGACCACAGCAATTCTGGGAAGCGAACTTACACAATGTATAGAATCACGGTTGCATTAACAAGT
  TTAAATTGTGTTGCTGATCCAATTCTGTACTGTTTTGTAACCGAAACAGGAAGATATGATATGTGGAATA
  TATTAAAATTCTGCACTGGGAGGTGTAATACATCACAAAGACAAAGAAAACGCATACTTTCTGTGTCTAC
  AAAAGATACTATGGAATTAGAGGTCCTTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207600 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNSTCIEEQHDLDHYLFPIVYIFVIIVSIPANIGSLCVSFLQAKKESELGIYLFSLSLSDLLYALTLPLW
  IDYTWNKDNWTFSPALCKGSAFLMYMNFYSSTAFLTCIAVDRYLAVVYPLKFFFLRTRRFALMVSLSIWI
  LETIFNAVMLWEDETVVEYCDAEKSNFTLCYDKYPLEKWQINLNLFRTCTGYAIPLVTILICNRKVYQAV
  RHNKATENKEKKRIIKLLVSITVTFVLCFTPFHVMLLIRCILEHAVNFEDHSNSGKRTYTMYRITVALTS
  LNCVADPILYCFVTETGRYDMWNILKFCTGRCNTSQRQRKRILSVSTKDTMELEVLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003608
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003608.1, NM_003608.2, NM_003608.3, NP_003599.2
• RefSeq Size: 4522 bp
• RefSeq ORF: 1014 bp
• Locus ID: 8477
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 39.3 kDa