Phosphorylase B (PHKB) (NM_001031835) Human Tagged ORF Clone

CAT#: RC207611

PHKB (Myc-DDK-tagged)-Human phosphorylase kinase, beta (PHKB), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY422202) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207611 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PHKB protein (Cat# TP307611). The protein was produced from HEK293T cells transfected with PHKB cDNA clone (Cat# RC207611) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PHKB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207611 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGCTCACCTGATGCAGTCGTCTCTCCGTCTTCCGCTTTCTTAAGGTCTGGCTCAGTTTATGAAC
  CTCTTAAAAGCATTAATCTTCCAAGACCTGATAATGAAACTCTCTGGGATAAGTTGGACCATTATTACAG
  AATTGTCAAGTCAACATTGCTGCTGTATCAAAGTCCAACTACCGGTCTCTTTCCCACTAAAACATGCGGT
  GGTGACCAGAAGGCCAAGATCCAGGACAGCCTATACTGCGCTGCTGGGGCCTGGGCTTTGGCTCTTGCAT
  ACAGGCGAATTGATGACGACAAGGGAAGGACCCATGAGCTGGAGCACTCAGCTATAAAATGCATGAGAGG
  AATTCTCTACTGCTATATGCGTCAGGCCGATAAGGTCCAGCAGTTTAAGCAGGATCCACGCCCAACAACA
  TGTCTTCACTCTGTTTTCAATGTGCATACAGGAGATGAGTTGCTTTCCTATGAGGAATATGGTCATCTTC
  AGATAAATGCAGTGTCACTTTATCTCCTTTACCTTGTGGAAATGATTTCCTCAGGACTCCAGATTATCTA
  CAACACTGATGAGGTCTCTTTTATTCAAAACCTTGTATTTTGTGTGGAAAGAGTTTACCGTGTGCCTGAC
  TTTGGTGTCTGGGAAAGAGGAAGCAAATATAATAATGGCAGCACAGAGCTACATTCGAGCTCGGTTGGTT
  TAGCAAAAGCAGCTCTAGAAGCAATTAATGGATTCAACCTTTTTGGCAACCAGGGCTGTTCGTGGTCAGT
  TATATTTGTGGATCTCGATGCTCACAATCGCAACAGGCAAACTTTGTGCTCGCTGTTACCCAGAGAATCA
  AGATCACATAATACAGATGCTGCCCTGCTCCCCTGCATCAGTTATCCTGCATTTGCCCTGGATGATGAAG
  TTCTTTTTAGCCAGACACTTGATAAAGTGGTTAGAAAATTAAAAGGAAAATATGGATTTAAACGTTTCTT
  GAGAGATGGGTATAGAACATCATTGGAAGATCCCAACAGATGCTACTACAAGCCAGCTGAAATTAAGCTA
  TTTGATGGCATTGAATGTGAATTTCCCATATTTTTCCTTTATATGATGATTGATGGAGTTTTTAGAGGCA
  ATCCTAAGCAAGTACAGGAATATCAGGATCTTTTGACTCCAGTACTTCATCATACCACAGAAGGATATCC
  TGTTGTACCAAAGTACTATTATGTGCCAGCTGACTTTGTAGAATATGAAAAAAATAACCCTGGTAGTCAA
  AAACGATTTCCTAGCAACTGTGGCCGTGATGGAAAACTGTTTCTTTGGGGACAAGCACTTTATATCATCG
  CAAAACTCCTGGCTGATGAACTTATTAGTCCTAAAGACATTGATCCTGTCCAGCGCTATGTCCCACTAAA
  GGATCAACGTAACGTGAGCATGAGGTTTTCCAATCAGGGCCCACTGGAAAATGACTTGGTAGTTCATGTG
  GCACTTATAGCAGAAAGCCAAAGACTTCAAGTTTTTCTGAACACATATGGTATTCAAACTCAAACTCCTC
  AACAAGTAGAACCCATTCAGATATGGCCTCAGCAGGAGCTTGTGAAAGCTTATTTGCAGCTGGGTATCAA
  TGAAAAGTTAGGACTCTCTGGAAGGCCAGACAGGCCCATTGGCTGCCTCGGGACATCAAAGATTTATCGC
  ATTCTAGGAAAGACTGTGGTTTGTTACCCGATTATTTTCGACCTAAGTGATTTCTACATGTCTCAGGATG
  TTTTCCTGCTGATAGATGACATAAAGAATGCGCTGCAGTTCATTAAACAATATTGGAAAATGCATGGACG
  TCCACTTTTCCTTGTTCTCATCCGGGAAGACAATATAAGAGGTAGCCGGTTCAACCCCATATTAGATATG
  CTGGCAGCCCTTAAAAAAGGAATAATTGGAGGAGTCAAAGTTCATGTGGATCGTCTACAGACACTAATAT
  CTGGAGCTGTGGTAGAACAACTTGATTTCCTACGAATCAGTGACACAGAAGAGCTTCCAGAATTTAAGAG
  TTTTGAGGAACTAGAACCTCCCAAACATTCAAAAGTCAAACGGCAAAGCAGCACCCCTAGTGCTCCTGAA
  CTGGGACAGCAGCCGGATGTCAACATTAGTGAATGGAAGGACAAACCCACCCATGAAATTCTTCAAAAAC
  TGAATGATTGCAGTTGTCTGGCTAGCCAAGCCATCCTGCTGGGTATACTGCTCAAAAGAGAAGGCCCCAA
  CTTCATCACAAAGGAAGGTACCGTTTCTGATCACATTGAGAGAGTCTATAGAAGAGCTGGCAGCCAAAAA
  CTTTGGTCGGTTGTACGCCGTGCAGCAAGTCTTTTAAGTAAAGTAGTGGACAGCCTGGCCCCATCCATTA
  CTAATGTTTTAGTGCAGGGCAAACAGGTAACTCTGGGTGCCTTTGGGCATGAAGAAGAAGTTATCTCTAA
  TCCTTTGTCTCCAAGAGTGATTCAAAACATCATCTATTATAAGTGTAACACCCATGATGAGAGGGAAGCG
  GTCATTCAGCAAGAACTGGTCATCCATATTGGCTGGATCATCTCCAATAACCCTGAGTTATTCAGTGGCA
  TGCTGAAAATACGAATCGGGTGGATCATCCATGCCATGGAGTATGAACTTCAGATCCGTGGCGGAGACAA
  GCCAGCCTTGGACTTGTATCAGCTGTCACCTAGTGAAGTTAAACAGCTTCTGCTGGATATTCTGCAGCCT
  CAACAGAATGGAAGATGTTGGCTGAACAGGCGTCAGATCGATGGGTCTTTGAATAGAACTCCCACCGGGT
  TCTATGACCGAGTGTGGCAGATTCTGGAGCGCACGCCCAATGGGATCATTGTTGCTGGGAAGCATTTGCC
  TCAGCAACCAACCCTGTCAGATATGACCATGTATGAGATGAATTTCTCTCTCCTTGTTGAAGACACGTTG
  GGAAATATTGACCAGCCACAGTACAGACAGATCGTTGTAGAGTTACTTATGGTTGTATCCATTGTACTGG
  AAAGAAACCCCGAGCTAGAATTTCAAGACAAAGTAGATCTAGACAGACTGGTCAAAGAAGCATTTAATGA
  ATTTCAAAAAGATCAGAGTCGGCTAAAGGAAATTGAAAAACAAGATGACATGACTTCCTTTTACAACACT
  CCTCCCCTGGGAAAAAGAGGAACATGCAGCTATTTGACAAAGGCGGTGATGAATCTGCTGCTGGAAGGAG
  AAGTCAAGCCAAACAATGATGACCCGTGTCTGATTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207611 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MACSPDAVVSPSSAFLRSGSVYEPLKSINLPRPDNETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCG
  GDQKAKIQDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTT
  CLHSVFNVHTGDELLSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPD
  FGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRES
  RSHNTDAALLPCISYPAFALDDEVLFSQTLDKVVRKLKGKYGFKRFLRDGYRTSLEDPNRCYYKPAEIKL
  FDGIECEFPIFFLYMMIDGVFRGNPKQVQEYQDLLTPVLHHTTEGYPVVPKYYYVPADFVEYEKNNPGSQ
  KRFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVQRYVPLKDQRNVSMRFSNQGPLENDLVVHV
  ALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLQLGINEKLGLSGRPDRPIGCLGTSKIYR
  ILGKTVVCYPIIFDLSDFYMSQDVFLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDM
  LAALKKGIIGGVKVHVDRLQTLISGAVVEQLDFLRISDTEELPEFKSFEELEPPKHSKVKRQSSTPSAPE
  LGQQPDVNISEWKDKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKEGTVSDHIERVYRRAGSQK
  LWSVVRRAASLLSKVVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPRVIQNIIYYKCNTHDEREA
  VIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPALDLYQLSPSEVKQLLLDILQP
  QQNGRCWLNRRQIDGSLNRTPTGFYDRVWQILERTPNGIIVAGKHLPQQPTLSDMTMYEMNFSLLVEDTL
  GNIDQPQYRQIVVELLMVVSIVLERNPELEFQDKVDLDRLVKEAFNEFQKDQSRLKEIEKQDDMTSFYNT
  PPLGKRGTCSYLTKAVMNLLLEGEVKPNNDDPCLIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031835
• ORF Size: 3258 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031835.1, NM_001031835.2, NP_001027005.1
• RefSeq Size: 5603 bp
• RefSeq ORF: 3261 bp
• Locus ID: 5257
• Cytogenetics: 16q12.1
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Insulin signaling pathway
• MW: 123.8 kDa
• Gene Summary: 'Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, encoded by this gene, which is a member of the phosphorylase b kinase regulatory subunit family. The gamma subunit also includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9B, also known as phosphorylase kinase deficiency of liver and muscle. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. Two pseudogenes have been found on chromosomes 14 and 20, respectively.[provided by RefSeq, Feb 2010]'