DBC2 (RHOBTB2) (NM_015178) Human Tagged ORF Clone

CAT#: RC207678

RHOBTB2 (Myc-DDK-tagged)-Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RHOBTB2
• Synonyms: DBC2; EIEE64
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207678 representing NM_015178
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTCTGACATGGATTATGAAAGGCCAAACGTAGAGACCATCAAGTGCGTTGTGGTGGGGGACAACG
  CCGTGGGTAAGACCAGGCTCATCTGTGCCCGCGCTTGCAATGCCACCCTCACCCAGTACCAGCTGCTGGC
  CACGCATGTGCCCACAGTATGGGCCATCGACCAATATCGTGTGTGCCAGGAGGTGCTGGAACGCTCCCGA
  GACGTGGTAGATGATGTCAGCGTCTCTCTGCGCCTCTGGGACACCTTTGGAGACCACCACAAAGACCGTC
  GCTTTGCTTATGGGAGATCTGATGTGGTGGTTCTGTGCTTCTCCATTGCCAACCCCAATTCCCTCCACCA
  TGTCAAGACCATGTGGTACCCAGAAATCAAGCACTTCTGCCCCCGAGCACCTGTCATCTTGGTGGGCTGC
  CAGTTGGACCTGCGCTACGCTGACCTGGAGGCTGTCAACAGGGCTAGGCGACCCTTGGCTAGGCCCATCA
  AACCTAATGAAATCCTGCCCCCAGAGAAGGGTCGGGAGGTGGCCAAGGAGCTGGGCATCCCCTACTATGA
  GACCAGCGTGGTGGCCCAGTTCGGCATCAAGGACGTCTTTGACAACGCCATCCGAGCTGCACTCATCTCC
  CGCCGCCACCTGCAGTTCTGGAAGTCCCACCTCCGCAATGTGCAGCGGCCTCTGCTGCAGGCACCCTTCC
  TACCCCCCAAGCCACCGCCCCCGATCATCGTGGTGCCCGACCCTCCCTCCAGCAGCGAGGAGTGCCCCGC
  CCACCTCCTGGAGGACCCGCTCTGCGCGGACGTCATCCTGGTGCTGCAGGAGCGGGTGCGCATCTTTGCC
  CACAAGATCTACCTCTCCACCTCTTCCTCCAAGTTCTATGACCTGTTCCTCATGGACCTGAGTGAGGGGG
  AGCTGGGGGGCCCCTCGGAGCCAGGGGGCACCCACCCAGAGGACCACCAGGGCCACTCTGATCAACACCA
  CCACCATCACCACCACCACCATGGGCGAGACTTCCTGCTCCGAGCAGCCAGCTTTGACGTGTGCGAGAGC
  GTGGATGAGGCTGGGGGCTCCGGTCCTGCTGGCCTCCGTGCTTCCACCAGCGACGGGATCTTACGGGGCA
  ACGGAACAGGGTACCTACCGGGCAGGGGTCGTGTGCTGTCTTCCTGGAGCCGAGCTTTTGTGAGCATCCA
  GGAAGAGATGGCAGAAGATCCTCTCACCTACAAATCCCGGCTGATGGTGGTGGTGAAGATGGACAGTTCC
  ATCCAGCCGGGGCCCTTCCGGGCTGTCCTCAAGTACCTGTACACGGGGGAGCTAGATGAGAACGAGCGTG
  ACCTCATGCACATTGCCCACATTGCTGAGCTGCTCGAGGTCTTTGATCTGCGCATGATGGTGGCCAACAT
  TCTCAACAATGAGGCCTTCATGAACCAGGAGATCACCAAGGCCTTCCACGTCCGCCGGACCAACCGGGTT
  AAGGAGTGCTTGGCAAAAGGCACCTTCTCAGATGTGACCTTCATCCTGGATGATGGCACCATCAGCGCCC
  ACAAGCCCCTGTTGATTTCCAGCTGTGACTGGATGGCTGCCATGTTTGGGGGGCCATTTGTGGAGAGCTC
  CACCCGGGAGGTGGTGTTTCCCTACACAAGCAAGAGCTGCATGCGGGCCGTGCTGGAATACCTCTACACC
  GGCATGTTCACCTCCAGCCCCGACCTGGATGACATGAAGCTCATCATTCTAGCCAACCGCCTCTGCCTGC
  CACACCTGGTTGCCCTCACAGAGCAGTACACAGTGACCGGGCTGATGGAAGCGACCCAGATGATGGTGGA
  CATCGATGGGGACGTCCTTGTGTTCCTGGAACTGGCTCAGTTCCACTGTGCGTACCAGCTGGCCGACTGG
  TGTCTCCACCACATCTGCACCAACTACAACAACGTGTGCCGCAAGTTCCCCCGAGACATGAAGGCCATGT
  CCCCAGAAAACCAGGAGTATTTCGAGAAGCATCGGTGGCCACCTGTGTGGTACCTGAAGGAGGAAGATCA
  TTACCAGCGGGCACGGAAGGAGCGTGAGAAGGAGGACTACCTCCACCTCAAGCGGCAGCCCAAACGGCGT
  TGGCTCTTCTGGAACAGTCCATCCTCCCCGTCTTCCTCGGCAGCCTCCTCCTCATCCCCATCTTCCTCCT
  CGGCTGTGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207678 representing NM_015178
  Red=Cloning site Green=Tags(s)
  
  MDSDMDYERPNVETIKCVVVGDNAVGKTRLICARACNATLTQYQLLATHVPTVWAIDQYRVCQEVLERSR
  DVVDDVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLHHVKTMWYPEIKHFCPRAPVILVGC
  QLDLRYADLEAVNRARRPLARPIKPNEILPPEKGREVAKELGIPYYETSVVAQFGIKDVFDNAIRAALIS
  RRHLQFWKSHLRNVQRPLLQAPFLPPKPPPPIIVVPDPPSSSEECPAHLLEDPLCADVILVLQERVRIFA
  HKIYLSTSSSKFYDLFLMDLSEGELGGPSEPGGTHPEDHQGHSDQHHHHHHHHHGRDFLLRAASFDVCES
  VDEAGGSGPAGLRASTSDGILRGNGTGYLPGRGRVLSSWSRAFVSIQEEMAEDPLTYKSRLMVVVKMDSS
  IQPGPFRAVLKYLYTGELDENERDLMHIAHIAELLEVFDLRMMVANILNNEAFMNQEITKAFHVRRTNRV
  KECLAKGTFSDVTFILDDGTISAHKPLLISSCDWMAAMFGGPFVESSTREVVFPYTSKSCMRAVLEYLYT
  GMFTSSPDLDDMKLIILANRLCLPHLVALTEQYTVTGLMEATQMMVDIDGDVLVFLELAQFHCAYQLADW
  CLHHICTNYNNVCRKFPRDMKAMSPENQEYFEKHRWPPVWYLKEEDHYQRARKEREKEDYLHLKRQPKRR
  WLFWNSPSSPSSSAASSSSPSSSSAVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015178
• ORF Size: 2181 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015178.1, NM_015178.2, NP_055993.1, NP_055993.2
• RefSeq Size: 5449
• RefSeq ORF: 2184
• Locus ID: 23221
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 82.6 kDa
• Gene Summary: The protein encoded by this gene is a small Rho GTPase and a candidate tumor suppressor. The encoded protein interacts with the cullin-3 protein, a ubiquitin E3 ligase necessary for mitotic cell division. This protein inhibits the growth and spread of some types of breast cancer. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]