H963 (GPR171) (NM_013308) Human Tagged ORF Clone

CAT#: RC207757

GPR171 (Myc-DDK-tagged)-Human G protein-coupled receptor 171 (GPR171)

Product Images

Coomassie blue staining of purified GPR171 protein (Cat# TP307757). The protein was produced from HEK293T cells transfected with GPR171 cDNA clone (Cat# RC207757) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPR171
• Synonyms: H963
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207757 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAACAGTTCGTTCTTCTGCCCAGTTTATAAAGATCTGGAGCCATTCACGTATTTTTTTTATTTAG
  TTTTCCTTGTTGGAATTATTGGAAGTTGTTTTGCAACCTGGGCTTTTATACAGAAGAATACGAATCACAG
  GTGTGTGAGCATCTACTTAATTAATTTGCTTACAGCCGATTTCCTGCTTACTCTGGCATTACCAGTGAAA
  ATTGTTGTTGACTTGGGTGTGGCACCTTGGAAGCTGAAGATATTCCACTGCCAAGTAACAGCCTGCCTCA
  TCTATATCAATATGTATTTATCAATTATCTTCTTAGCATTTGTCAGCATTGACCGCTGTCTTCAGCTGAC
  ACACAGCTGCAAGATCTACCGAATACAAGAACCCGGATTTGCCAAAATGATATCAACCGTTGTGTGGCTA
  ATGGTCCTTCTTATAATGGTGCCAAATATGATGATTCCCATCAAAGACATCAAGGAAAAGTCAAATGTGG
  GTTGTATGGAGTTTAAAAAGGAATTTGGAAGAAATTGGCATTTGCTGACAAATTTCATATGTGTAGCAAT
  ATTTTTAAATTTCTCAGCCATCATTTTAATATCCAATTGCCTTGTAATTCGACAGCTCTACAGAAACAAA
  GATAATGAAAATTACCCAAATGTGAAAAAGGCTCTCATCAACATACTTTTAGTGACCACGGGCTACATCA
  TATGCTTTGTTCCTTACCACATTGTCCGAATCCCGTATACCCTCAGCCAGACAGAAGTCATAACTGATTG
  CTCAACCAGGATTTCACTCTTCAAAGCCAAAGAGGCTACACTGCTCCTGGCTGTGTCGAACCTGTGCTTT
  GATCCTGTCCTGTACTATCACCTCTCAAAAGCATTCCGCTCAAAGGTCACTGAGACTTTTGCCTCACCTA
  AAGAGACCAAGGCTCAGAAAGAAAAATTAAGATGTGAAAATAATGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207757 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTNSSFFCPVYKDLEPFTYFFYLVFLVGIIGSCFATWAFIQKNTNHRCVSIYLINLLTADFLLTLALPVK
  IVVDLGVAPWKLKIFHCQVTACLIYINMYLSIIFLAFVSIDRCLQLTHSCKIYRIQEPGFAKMISTVVWL
  MVLLIMVPNMMIPIKDIKEKSNVGCMEFKKEFGRNWHLLTNFICVAIFLNFSAIILISNCLVIRQLYRNK
  DNENYPNVKKALINILLVTTGYIICFVPYHIVRIPYTLSQTEVITDCSTRISLFKAKEATLLLAVSNLCF
  DPVLYYHLSKAFRSKVTETFASPKETKAQKEKLRCENNA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013308
• ORF Size: 957 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013308.1, NM_013308.2, NM_013308.3, NP_037440.2
• RefSeq Size: 1862 bp
• RefSeq ORF: 960 bp
• Locus ID: 29909
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 36.7 kDa
• Gene Summary: Orphan receptor. [UniProtKB/Swiss-Prot Function]