GRHL3 (NM_198174) Human Tagged ORF Clone
CAT#: RC207857
GRHL3 (Myc-DDK-tagged)-Human grainyhead-like 3 (Drosophila) (GRHL3), transcript variant 3
"NM_198174" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | GRHL3 |
Synonyms | SOM; TFCP2L4; VWS2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207857 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGTCAATGGAGATGATGACAGTGTTGCGGCCTTGAGCTTCCTCTATGATTACTACATGGGTCCCA AGGAGAAGCGGATATTGTCCTCCAGCACTGGGGGCAGGAATGACCAAGGAAAGAGGTACTACCATGGCAT GGAATATGAGACGGACCTCACTCCCCTTGAAAGCCCCACACACCTCATGAAATTCCTGACAGAGAACGTG TCTGGAACCCCAGAGTACCCAGATTTGCTCAAGAAGAATAACCTGATGAGCTTGGAGGGGGCCTTGCCCA CCCCTGGCAAGGCAGCTCCCCTCCCTGCAGGCCCCAGCAAGCTGGAGGCCGGCTCTGTGGACAGCTACCT GTTACCCACCACTGATATGTATGATAATGGCTCCCTCAACTCCTTGTTTGAGAGCATTCATGGGGTGCCG CCCACACAGCGCTGGCAGCCAGACAGCACCTTCAAAGATGACCCACAGGAGTCGATGCTCTTCCCAGATA TCCTGAAAACCTCCCCGGAACCCCCATGTCCAGAGGACTACCCCAGCCTCAAAAGTGACTTTGAATACAC CCTGGGCTCCCCCAAAGCCATCCACATCAAGTCAGGCGAGTCACCCATGGCCTACCTCAACAAAGGCCAG TTCTACCCCGTCACCCTGCGGACCCCAGCAGGTGGCAAAGGCCTTGCCTTGTCCTCCAACAAAGTCAAGA GTGTGGTGATGGTTGTCTTCGACAATGAGAAGGTCCCAGTAGAGCAGCTGCGCTTCTGGAAGCACTGGCA TTCCCGGCAACCCACTGCCAAGCAGCGGGTCATTGACGTGGCTGACTGCAAAGAAAACTTCAACACTGTG GAGCACATTGAGGAGGTGGCCTATAATGCACTGTCCTTTGTGTGGAACGTGAATGAAGAGGCCAAGGTGT TCATCGGCGTAAACTGTCTGAGCACAGACTTTTCCTCACAAAAGGGGGTGAAGGGTGTCCCCCTGAACCT GCAGATTGACACCTATGACTGTGGCTTGGGCACTGAGCGCCTGGTACACCGTGCTGTCTGCCAGATCAAG ATCTTCTGTGACAAGGGAGCTGAGAGGAAGATGCGCGATGACGAGCGGAAGCAGTTCCGGAGGAAGGTCA AGTGCCCTGACTCCAGCAACAGTGGCGTCAAGGGCTGCCTGCTGTCGGGCTTCAGGGGCAATGAGACGAC CTACCTTCGGCCAGAGACTGACCTGGAGACGCCACCCGTGCTGTTCATCCCCAATGTGCACTTCTCCAGC CTGCAGCGCTCTGGAGGGGCAGCCCCCTCGGCAGGACCCAGCAGCTCCAACAGGCTGCCTCTGAAGCGTA CCTGCTCGCCCTTCACTGAGGAGTTTGAGCCTCTGCCCTCCAAGCAGGCCAAGGAAGGCGACCTTCAGAG AGTTCTGCTGTATGTGCGGAGGGAGACTGAGGAGGTGTTTGACGCGCTCATGTTGAAGACCCCAGACCTG AAGGGGCTGAGGAATGCGATCTCTGAGAAGTATGGGTTCCCTGAAGAGAACATTTACAAAGTCTACAAGA AATGCAAGCGAGGAATCTTAGTCAACATGGACAACAACATCATTCAGCATTACAGCAACCACGTCGCCTT CCTGCTGGACATGGGGGAGCTGGACGGCAAAATTCAGATCATCCTTAAGGAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207857 protein sequence
Red=Cloning site Green=Tags(s) MRVNGDDDSVAALSFLYDYYMGPKEKRILSSSTGGRNDQGKRYYHGMEYETDLTPLESPTHLMKFLTENV SGTPEYPDLLKKNNLMSLEGALPTPGKAAPLPAGPSKLEAGSVDSYLLPTTDMYDNGSLNSLFESIHGVP PTQRWQPDSTFKDDPQESMLFPDILKTSPEPPCPEDYPSLKSDFEYTLGSPKAIHIKSGESPMAYLNKGQ FYPVTLRTPAGGKGLALSSNKVKSVVMVVFDNEKVPVEQLRFWKHWHSRQPTAKQRVIDVADCKENFNTV EHIEEVAYNALSFVWNVNEEAKVFIGVNCLSTDFSSQKGVKGVPLNLQIDTYDCGLGTERLVHRAVCQIK IFCDKGAERKMRDDERKQFRRKVKCPDSSNSGVKGCLLSGFRGNETTYLRPETDLETPPVLFIPNVHFSS LQRSGGAAPSAGPSSSNRLPLKRTCSPFTEEFEPLPSKQAKEGDLQRVLLYVRRETEEVFDALMLKTPDL KGLRNAISEKYGFPEENIYKVYKKCKRGILVNMDNNIIQHYSNHVAFLLDMGELDGKIQIILKEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_198174 |
ORF Size | 1668 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 2232 bp |
RefSeq ORF | 1881 bp |
Locus ID | 57822 |
MW | 62.2 kDa |
Gene Summary | This gene encodes a member of the grainyhead family of transcription factors. The encoded protein may function as a transcription factor during development, and has been shown to stimulate migration of endothelial cells. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2010] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC321864 | GRHL3 (untagged)-Human grainyhead-like 3 (Drosophila) (GRHL3), transcript variant 3 |
USD 760.00 |
|
RG207857 | GRHL3 (GFP-tagged) - Human grainyhead-like 3 (Drosophila) (GRHL3), transcript variant 3 |
USD 530.00 |
|
RC207857L3 | Lenti ORF clone of Human grainyhead-like 3 (Drosophila) (GRHL3), transcript variant 3, Myc-DDK-tagged |
USD 680.00 |
|
RC207857L4 | Lenti ORF clone of Human grainyhead-like 3 (Drosophila) (GRHL3), transcript variant 3, mGFP tagged |
USD 680.00 |
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