AXUD1 (CSRNP1) (NM_033027) Human Tagged ORF Clone

CAT#: RC207875

CSRNP1 (Myc-DDK-tagged)-Human cysteine-serine-rich nuclear protein 1 (CSRNP1)

Product Images

Western blot validation of overexpression lysate (Cat# LY409787) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207875 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CSRNP1
• Synonyms: AXUD1; CSRNP-1; FAM130B; TAIP-3; URAX1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207875 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGGGCTGTTGAAGAGGAAATTTGACCAGCTGGATGAGGACAACTCCTCGGTCTCCTCCTCCTCCT
  CTTCCTCTGGGTGCCAGTCTCGCTCCTGCTCCCCAAGCTCTTCTGTCTCCCGTGCCTGGGACTCAGAGGA
  GGAAGGCCCCTGGGATCAGATGCCCCTGCCTGACCGTGACTTCTGCGGCCCCAGAAGTTTCACCCCCCTG
  TCTATCCTGAAGCGAGCTCGCCGGGAGCGCCCAGGCCGTGTAGCCTTTGATGGGATCACCGTCTTCTACT
  TCCCCCGCTGCCAGGGCTTCACCAGTGTGCCCAGCCGTGGTGGCTGTACTCTGGGTATGGCCCTTCGCCA
  CAGTGCTTGCCGTCGCTTCTCTTTGGCTGAGTTTGCGCAGGAGCAAGCCCGTGCACGGCACGAGAAGCTC
  CGCCAGCGCTTGAAAGAGGAGAAGTTGGAGATGCTGCAGTGGAAGCTTTCGGCAGCTGGGGTACCCCAGG
  CAGAGGCAGGGCTGCCACCTGTGGTGGATGCCATTGATGACGCCTCTGTGGAGGAGGACTTGGCAGTCGC
  TGTGGCAGGTGGCCGGTTGGAAGAAGTGAGCTTCCTACAGCCCTACCCAGCCCGGCGACGTCGAGCTCTG
  CTGAGGGCTTCAGGTGTGCGAAGGATCGATCGGGAGGAGAAGCGGGAGCTGCAGGCACTGCGCCAATCCC
  GGGAGGATTGTGGCTGTCACTGCGATAGGATCTGCGACCCTGAGACCTGCAGCTGCAGCCTGGCAGGCAT
  CAAGTGCCAGATGGACCACACAGCATTCCCCTGTGGCTGCTGCAGGGAGGGCTGTGAGAACCCCATGGGC
  CGTGTGGAATTTAATCAGGCAAGAGTTCAGACCCATTTCATCCACACACTCACCCGCCTGCAGTTGGAAC
  AGGAGGCTGAGAGCTTTAGGGAGCTGGAGGCCCCTGCCCAGGGCAGCCCACCCAGCCCTGGTGAGGAGGC
  CCTGGTCCCTACTTTCCCACTGGCCAAGCCCCCCATGAACAATGAGCTGGGAGACAACAGCTGCAGCAGC
  GACATGACTGATTCTTCCACAGCATCTTCATCAGCATCGGGCACTAGTGAGGCTCCTGACTGCCCCACCC
  ACCCAGGCCTGCCTGGCCCTGGCTTCCAGCCTGGCGTTGATGATGACAGCCTGGCACGCATCTTGAGTTT
  CAGTGACTCTGACTTCGGTGGGGAGGAGGAGGAAGAGGAGGAAGGGAGTGTGGGGAACCTGGACAACCTC
  AGCTGCTTCCATCCAGCTGACATCTTTGGTACTAGTGACCCTGGTGGCCTGGCCAGCTGGACCCACAGCT
  ATTCTGGCTGTAGCTTCACATCAGGCATCCTGGATGAGAATGCCAACCTGGATGCCAGCTGCTTCCTAAA
  TGGTGGCCTTGAAGGGTCAAGGGAAGGCAGCCTTCCTGGCACCTCAGTGCCACCCAGCATGGACGCTGGC
  CGGAGTAGCTCAGTGGATCTCAGCTTGTCTTCTTGTGACTCCTTTGAGTTACTCCAGGCTCTGCCAGATT
  ATAGTCTGGGGCCTCACTACACATCACAGAAGGTGTCTGACAGCCTGGACAACATCGAGGCACCTCACTT
  CCCCCTGCCTGGCCTGTCTCCACCTGGGGATGCCAGCAGTTGCTTCCTGGAGTCCCTCATGGGCTTCTCC
  GAGCCAGCCGCCGAAGCCCTAGATCCCTTTATTGACAGCCAGTTTGAGGACACTGTCCCAGCATCTCTAA
  TGGAGCCTGTGCCGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207875 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTGLLKRKFDQLDEDNSSVSSSSSSSGCQSRSCSPSSSVSRAWDSEEEGPWDQMPLPDRDFCGPRSFTPL
  SILKRARRERPGRVAFDGITVFYFPRCQGFTSVPSRGGCTLGMALRHSACRRFSLAEFAQEQARARHEKL
  RQRLKEEKLEMLQWKLSAAGVPQAEAGLPPVVDAIDDASVEEDLAVAVAGGRLEEVSFLQPYPARRRRAL
  LRASGVRRIDREEKRELQALRQSREDCGCHCDRICDPETCSCSLAGIKCQMDHTAFPCGCCREGCENPMG
  RVEFNQARVQTHFIHTLTRLQLEQEAESFRELEAPAQGSPPSPGEEALVPTFPLAKPPMNNELGDNSCSS
  DMTDSSTASSSASGTSEAPDCPTHPGLPGPGFQPGVDDDSLARILSFSDSDFGGEEEEEEEGSVGNLDNL
  SCFHPADIFGTSDPGGLASWTHSYSGCSFTSGILDENANLDASCFLNGGLEGSREGSLPGTSVPPSMDAG
  RSSSVDLSLSSCDSFELLQALPDYSLGPHYTSQKVSDSLDNIEAPHFPLPGLSPPGDASSCFLESLMGFS
  EPAAEALDPFIDSQFEDTVPASLMEPVPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033027
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033027.1, NM_033027.2, NM_033027.3, NP_149016.1
• RefSeq Size: 3188 bp
• RefSeq ORF: 1770 bp
• Locus ID: 64651
• MW: 63.5 kDa
• Gene Summary: This gene encodes a protein that localizes to the nucleus and expression of this gene is induced in response to elevated levels of axin. The Wnt signalling pathway, which is negatively regulated by axin, is important in axis formation in early development and impaired regulation of this signalling pathway is often involved in tumors. A decreased level of expression of this gene in tumors compared to the level of expression in their corresponding normal tissues suggests that this gene product has a tumor suppressor function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]