GCLC (NM_001498) Human Tagged ORF Clone

CAT#: RC207915

GCLC (Myc-DDK-tagged)-Human glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY419901) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207915 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified GCLC protein (Cat# TP307915). The protein was produced from HEK293T cells transfected with GCLC cDNA clone (Cat# RC207915) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GCLC
• Synonyms: GCL; GCS; GLCL; GLCLC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207915 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGCTGTCCCAGGGCTCGCCGCTGAGCTGGGAGGAAACCAAGCGCCATGCCGACCACGTGCGGC
  GGCACGGGATCCTCCAGTTCCTGCACATCTACCACGCCGTCAAGGACCGGCACAAGGACGTTCTCAAGTG
  GGGCGATGAGGTGGAATACATGTTGGTATCTTTTGATCATGAAAATAAAAAAGTCCGGTTGGTCCTGTCT
  GGGGAGAAAGTTCTTGAAACTCTGCAAGAGAAGGGGGAAAGGACAAACCCAAACCATCCTACCCTTTGGA
  GACCAGAGTATGGGAGTTACATGATTGAAGGGACACCAGGACAGCCCTACGGAGGAACAATGTCCGAGTT
  CAATACAGTTGAGGCCAACATGCGAAAACGCCGGAAGGAGGCTACTTCTATATTAGAAGAAAATCAGGCT
  CTTTGCACAATAACTTCATTTCCCAGATTAGGCTGTCCTGGGTTCACACTGCCCGAGGTCAAACCCAACC
  CAGTGGAAGGAGGAGCTTCCAAGTCCCTCTTCTTTCCAGATGAAGCAATAAACAAGCACCCTCGCTTCAG
  TACCTTAACAAGAAATATCCGACATAGGAGAGGAGAAAAGGTTGTCATCAATGTACCAATATTTAAGGAC
  AAGAATACACCATCTCCATTTATAGAAACATTTACTGAGGATGATGAAGCTTCAAGGGCTTCTAAGCCGG
  ATCATATTTACATGGATGCCATGGGATTTGGAATGGGCAATTGCTGTCTCCAGGTGACATTCCAAGCCTG
  CAGTATATCTGAGGCCAGATACCTTTATGATCAGTTGGCTACTATCTGTCCAATTGTTATGGCTTTGAGT
  GCTGCATCTCCCTTTTACCGAGGCTATGTGTCAGACATTGATTGTCGCTGGGGAGTGATTTCTGCATCTG
  TAGATGATAGAACTCGGGAGGAGCGAGGACTGGAGCCATTGAAGAACAATAACTATAGGATCAGTAAATC
  CCGATATGACTCAATAGACAGCTATTTATCTAAGTGTGGTGAGAAATATAATGACATCGACTTGACGATA
  GATAAAGAGATCTACGAACAGCTGTTGCAGGAAGGCATTGATCATCTCCTGGCCCAGCATGTTGCTCATC
  TCTTTATTAGAGACCCACTGACACTGTTTGAAGAGAAAATACACCTGGATGATGCTAATGAGTCTGACCA
  TTTTGAGAATATTCAGTCCACAAATTGGCAGACAATGAGATTTAAGCCCCCTCCTCCAAACTCAGACATT
  GGATGGAGAGTAGAATTTCGACCCATGGAGGTGCAATTAACAGACTTTGAGAACTCTGCCTATGTGGTGT
  TTGTGGTACTGCTCACCAGAGTGATCCTTTCCTACAAATTGGATTTTCTCATTCCACTGTCAAAGGTTGA
  TGAGAACATGAAGGTAGCACAGAAAAGAGATGCTGTCTTGCAGGGAATGTTTTATTTCAGGAAAGATATT
  TGCAAAGGTGGCAATGCAGTGGTGGATGGTTGTGGCAAGGCCCAGAACAGCACGGAGCTCGCTGCAGAGG
  AGTACACCCTCATGAGCATAGACACCATCATCAATGGGAAGGAAGGTGTGTTTCCTGGACTGATCCCAAT
  TCTGAACTCTTACCTTGAAAACATGGAAGTGGATGTGGACACCAGATGTAGTATTCTGAACTACCTAAAG
  CTAATTAAGAAGAGAGCATCTGGAGAACTAATGACAGTTGCCAGATGGATGAGGGAGTTTATCGCAAACC
  ATCCTGACTACAAGCAAGACAGTGTCATAACTGATGAAATGAATTATAGCCTTATTTTGAAGTGTAACCA
  AATTGCAAATGAATTATGTGAATGCCCAGAGTTACTTGGATCAGCATTTAGGAAAGTAAAATATAGTGGA
  AGTAAAACTGACTCATCCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207915 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVRLVLS
  GEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQA
  LCTITSFPRLGCPGFTLPEVKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKD
  KNTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALS
  AASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNNYRISKSRYDSIDSYLSKCGEKYNDIDLTI
  DKEIYEQLLQEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDI
  GWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQGMFYFRKDI
  CKGGNAVVDGCGKAQNSTELAAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLK
  LIKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELLGSAFRKVKYSG
  SKTDSSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001498
• ORF Size: 1911 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001498.1, NM_001498.2, NM_001498.3, NP_001489.1
• RefSeq Size: 3823 bp
• RefSeq ORF: 1914 bp
• Locus ID: 2729
• Cytogenetics: 6p12.1
• Domains: GCS
• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism, Metabolic pathways
• MW: 72.8 kDa
• Gene Summary: 'Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]'