P2X1 (P2RX1) (NM_002558) Human Tagged ORF Clone

CAT#: RC207924

P2RX1 (Myc-DDK-tagged)-Human purinergic receptor P2X, ligand-gated ion channel, 1 (P2RX1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: P2RX1
• Synonyms: P2X1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207924 representing NM_002558
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACGGCGGTTCCAGGAGGAGCTGGCCGCCTTCCTCTTCGAGTATGACACCCCCCGCATGGTGCTGG
  TGCGTAATAAGAAGGTGGGCGTTATCTTCCGACTGATCCAGCTGGTGGTCCTGGTCTACGTCATCGGGTG
  GGTGTTTCTCTATGAGAAGGGCTACCAGACCTCGAGCGGCCTCATCAGCAGTGTCTCTGTGAAACTCAAG
  GGCCTGGCCGTGACCCAGCTCCCTGGCCTCGGCCCCCAGGTCTGGGATGTGGCTGACTACGTCTTCCCAG
  CCCAGGGGGACAACTCCTTCGTGGTCATGACCAATTTCATCGTGACCCCGAAGCAGACTCAAGGCTACTG
  CGCAGAGCACCCAGAAGGGGGCATATGCAAGGAAGACAGTGGCTGTACCCCTGGGAAGGCCAAGAGGAAG
  GCCCAAGGCATCCGCACGGGCAAGTGTGTGGCCTTCAACGACACTGTGAAGACGTGTGAGATCTTTGGCT
  GGTGCCCCGTGGAGGTGGATGACGACATCCCGCGCCCTGCCCTTCTCCGAGAGGCCGAGAACTTCACTCT
  TTTCATCAAGAACAGCATCAGCTTTCCACGCTTCAAGGTCAACAGGCGCAACCTGGTGGAGGAGGTGAAT
  GCTGCCCACATGAAGACCTGCCTCTTTCACAAGACCCTGCACCCCCTGTGCCCAGTCTTCCAGCTTGGCT
  ACGTGGTGCAAGAGTCAGGCCAGAACTTCAGCACCCTGGCTGAGAAGGGTGGAGTGGTTGGCATCACCAT
  CGACTGGCACTGTGACCTGGACTGGCACGTACGGCACTGCAGACCCATCTATGAGTTCCATGGGCTGTAC
  GAAGAGAAAAATCTCTCCCCAGGCTTCAACTTCAGGTTTGCCAGGCACTTTGTGGAGAACGGGACCAACT
  ACCGTCACCTCTTCAAGGTGTTTGGGATTCGCTTTGACATCCTGGTGGACGGCAAGGCCGGGAAGTTTGA
  CATCATCCCTACAATGACCACCATCGGCTCTGGAATTGGCATCTTTGGGGTGGCCACAGTTCTCTGTGAC
  CTGCTGCTGCTTCACATCCTGCCTAAGAGGCACTACTACAAGCAGAAGAAGTTCAAATACGCTGAGGACA
  TGGGGCCAGGGGCGGCTGAGCGTGACCTCGCAGCTACCAGCTCCACCCTGGGCCTGCAGGAGAACATGAG
  GACATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207924 representing NM_002558
  Red=Cloning site Green=Tags(s)
  
  MARRFQEELAAFLFEYDTPRMVLVRNKKVGVIFRLIQLVVLVYVIGWVFLYEKGYQTSSGLISSVSVKLK
  GLAVTQLPGLGPQVWDVADYVFPAQGDNSFVVMTNFIVTPKQTQGYCAEHPEGGICKEDSGCTPGKAKRK
  AQGIRTGKCVAFNDTVKTCEIFGWCPVEVDDDIPRPALLREAENFTLFIKNSISFPRFKVNRRNLVEEVN
  AAHMKTCLFHKTLHPLCPVFQLGYVVQESGQNFSTLAEKGGVVGITIDWHCDLDWHVRHCRPIYEFHGLY
  EEKNLSPGFNFRFARHFVENGTNYRHLFKVFGIRFDILVDGKAGKFDIIPTMTTIGSGIGIFGVATVLCD
  LLLLHILPKRHYYKQKKFKYAEDMGPGAAERDLAATSSTLGLQENMRTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002558
• ORF Size: 1197 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_002558.1, NM_002558.2, NM_002558.3, NP_002549.1
• RefSeq Size: 2885
• RefSeq ORF: 1200
• Locus ID: 5023
• Domains: P2X_receptor
• Protein Families: Druggable Genome, Ion Channels: ATP Receptors, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• MW: 45.4 kDa
• Gene Summary: The protein encoded by this gene belongs to the P2X family of G-protein-coupled receptors. These proteins can form homo-and heterotimers and function as ATP-gated ion channels and mediate rapid and selective permeability to cations. This protein is primarily localized to smooth muscle where binds ATP and mediates synaptic transmission between neurons and from neurons to smooth muscle and may being responsible for sympathetic vasoconstriction in small arteries, arterioles and vas deferens. Mouse studies suggest that this receptor is essential for normal male reproductive function. This protein may also be involved in promoting apoptosis. [provided by RefSeq, Jun 2013]