Pirh2 (RCHY1) (NM_015436) Human Tagged ORF Clone
CAT#: RC207970
RCHY1 (Myc-DDK-tagged)-Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1
"NM_015436" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | RCHY1 |
Synonyms | ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY; ZNF363 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207970 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGACGGCCCGGGAAGATGGCGCCAGCGGTCAAGAGCGAGGTCAGCGGGGCTGCGAGCACTATG ACAGAGGATGTCTCCTAAAGGCACCTTGCTGTGACAAGCTTTATACTTGCCGCTTGTGTCATGATAACAA TGAAGATCATCAACTAGATCGCTTTAAAGTGAAGGAAGTGCAGTGCATAAACTGTGAAAAAATTCAACAT GCCCAACAGACTTGTGAAGAATGTAGCACATTGTTTGGAGAATATTATTGCGATATATGCCATTTGTTTG ACAAAGATAAGAAGCAGTATCACTGTGAAAACTGTGGAATTTGTAGGATTGGTCCAAAGGAAGATTTTTT CCATTGTTTGAAATGTAACTTATGCCTAGCTATGAATCTTCAAGGAAGACACAAGTGTATTGAAAATGTG TCCCAACAGAATTGTCCAATATGTTTGGAGGACATTCACACATCCCGTGTTGTTGCTCATGTCTTGCCAT GTGGACATCTTTTACATAGAACGTGTTATGAAGAAATGTTGAAAGAAGGCTACAGATGTCCATTATGTAT GCACTCTGCTTTAGATATGACCAGGTATTGGAGACAGCTGGATGATGAAGTAGCACAGACTCCTATGCCA TCAGAATATCAGAACATGACTGTGGATATTCTCTGCAATGACTGTAATGGACGATCCACTGTTCAGTTTC ATATATTAGGCATGAAATGTAAGATTTGTGAATCCTATAATACTGCTCAAGCTGGAGGACGTAGAATTTC ACTGGATCAGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207970 protein sequence
Red=Cloning site Green=Tags(s) MAATAREDGASGQERGQRGCEHYDRGCLLKAPCCDKLYTCRLCHDNNEDHQLDRFKVKEVQCINCEKIQH AQQTCEECSTLFGEYYCDICHLFDKDKKQYHCENCGICRIGPKEDFFHCLKCNLCLAMNLQGRHKCIENV SQQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLKEGYRCPLCMHSALDMTRYWRQLDDEVAQTPMP SEYQNMTVDILCNDCNGRSTVQFHILGMKCKICESYNTAQAGGRRISLDQQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015436 |
ORF Size | 783 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015436.1, NM_015436.2, NM_015436.3, NP_056251.2 |
RefSeq Size | 4447 bp |
RefSeq ORF | 786 bp |
Locus ID | 25898 |
Domains | RING |
Protein Families | Druggable Genome, Stem cell - Pluripotency |
Protein Pathways | p53 signaling pathway, Ubiquitin mediated proteolysis |
MW | 30.1 kDa |
Gene Summary | The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2013] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC107968 | RCHY1 (untagged)-Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1 |
USD 310.00 |
|
RG207970 | RCHY1 (GFP-tagged) - Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1 |
USD 460.00 |
|
RC207970L1 | Lenti ORF clone of Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC207970L2 | Lenti ORF clone of Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC207970L3 | Lenti ORF clone of Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC207970L4 | Lenti ORF clone of Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1, mGFP tagged |
USD 620.00 |
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