COG6 (NM_020751) Human Tagged ORF Clone

CAT#: RC207981

COG6 (Myc-DDK-tagged)-Human component of oligomeric golgi complex 6 (COG6), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY412375) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207981 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified COG6 protein (Cat# TP307981). The protein was produced from HEK293T cells transfected with COG6 cDNA clone (Cat# RC207981) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: COG6
• Synonyms: CDG2L; COD2; SHNS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207981 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGGGCAGCGGGGAAGTGGTCGCAGTGTCTGCGACCGGGGCTGCCAACGGCCTCAACAATGGGG
  CAGGCGGGACCTCGGCGACGACCTGCAACCCGCTGTCGCGCAAGCTGCATAAGATCCTGGAGACGCGGCT
  GGACAACGACAAGGAGATGTTAGAAGCTCTCAAGGCACTTTCAACCTTTTTTGTTGAAAATAGTCTGCGG
  ACTCGAAGAAATTTACGTGGAGATATTGAACGTAAAAGTTTAGCCATCAATGAAGAATTTGTAAGCATTT
  TCAAGGAAGTGAAGGAGGAACTTGAAAGCATAAGCGAAGATGTTCAAGCAATGAGCAACTGTTGTCAAGA
  TATGACAAGTCGCCTACAGGCAGCAAAGGAACAGACTCAAGATTTAATAGTAAAAACCACTAAGCTTCAA
  TCTGAAAGCCAAAAATTAGAGATAAGAGCTCAAGTTGCAGATGCCTTCTTATCCAAGTTCCAACTGACTT
  CTGATGAAATGAGTCTTCTCCGAGGTACAAGAGAAGGACCCATTACTGAGGATTTTTTCAAGGCACTGGG
  AAGAGTAAAACAGATTCATAATGATGTCAAAGTTCTCTTGCGTACAAATCAACAAACGGCAGGTTTAGAA
  ATTATGGAACAGATGGCCTTACTTCAAGAAACGGCTTATGAAAGACTTTACCGATGGGCTCAAAGTGAAT
  GCAGAACATTGACACAAGAATCATGTGACGTATCTCCGGTATTGACACAGGCAATGGAAGCCCTGCAGGA
  CAGACCTGTCTTATATAAATATACCTTAGATGAATTTGGAACAGCCAGAAGAAGTACAGTTGTTCGTGGA
  TTTATTGATGCGCTCACAAGAGGGGGCCCCGGAGGTACACCTAGACCAATTGAAATGCATTCTCATGACC
  CTTTGAGGTATGTAGGAGATATGTTGGCTTGGCTCCATCAAGCTACTGCTTCTGAAAAGGAACACCTTGA
  AGCTCTCTTAAAGCATGTAACTACACAAGGTGTTGAAGAAAATATTCAAGAAGTTGTTGGGCATATCACT
  GAAGGTGTGTGCAGGCCTCTAAAGGTTCGAATTGAGCAAGTAATAGTTGCTGAACCTGGGGCAGTTTTAT
  TATATAAAATTTCTAATCTCCTCAAATTTTATCACCATACAATCAGTGGTATTGTTGGAAATAGTGCAAC
  TGCATTATTGACTACCATTGAAGAAATGCATTTGCTAAGCAAAAAAATATTCTTCAATAGCTTGAGTCTT
  CATGCAAGTAAATTAATGGACAAGGTTGAACTCCCACCACCTGATCTTGGACCAAGTTCTGCACTAAATC
  AGACACTCATGTTGCTGCGTGAAGTTTTAGCATCTCACGATTCTTCAGTTGTACCATTAGATGCTCGTCA
  AGCTGATTTTGTGCAGGTTTTATCATGTGTCTTGGATCCTCTCCTACAGATGTGTACTGTATCAGCCAGC
  AATTTAGGCACAGCTGACATGGCCACTTTCATGGTCAATTCACTATATATGATGAAGACAACATTAGCTC
  TATTTGAATTCACTGACAGACGTCTGGAAATGCTACAGTTTCAGATCGAAGCACATTTGGACACACTTAT
  AAATGAGCAAGCCTCTTATGTTTTAACTAGGGTAGGCTTGAGTTACATCTATAACACTGTACAGCAACAT
  AAACCTGAACAGGGCTCTTTAGCTAATATGCCCAACCTAGATTCTGTGACACTGAAGGCTGCAATGGTTC
  AGTTTGATCGTTATCTGTCAGCCCCAGACAACCTATTGATACCACAGCTGAACTTTCTTCTAAGTGCCAC
  AGTGAAAGAGCAGATCGTAAAACAATCTACAGAATTAGTCTGCAGAGCCTATGGTGAAGTGTATGCAGCC
  GTGATGAATCCAATCAATGAATACAAAGATCCAGAGAACATTCTTCACCGATCGCCGCAGCAAGTGCAGA
  CGCTTCTTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207981 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEGSGEVVAVSATGAANGLNNGAGGTSATTCNPLSRKLHKILETRLDNDKEMLEALKALSTFFVENSLR
  TRRNLRGDIERKSLAINEEFVSIFKEVKEELESISEDVQAMSNCCQDMTSRLQAAKEQTQDLIVKTTKLQ
  SESQKLEIRAQVADAFLSKFQLTSDEMSLLRGTREGPITEDFFKALGRVKQIHNDVKVLLRTNQQTAGLE
  IMEQMALLQETAYERLYRWAQSECRTLTQESCDVSPVLTQAMEALQDRPVLYKYTLDEFGTARRSTVVRG
  FIDALTRGGPGGTPRPIEMHSHDPLRYVGDMLAWLHQATASEKEHLEALLKHVTTQGVEENIQEVVGHIT
  EGVCRPLKVRIEQVIVAEPGAVLLYKISNLLKFYHHTISGIVGNSATALLTTIEEMHLLSKKIFFNSLSL
  HASKLMDKVELPPPDLGPSSALNQTLMLLREVLASHDSSVVPLDARQADFVQVLSCVLDPLLQMCTVSAS
  NLGTADMATFMVNSLYMMKTTLALFEFTDRRLEMLQFQIEAHLDTLINEQASYVLTRVGLSYIYNTVQQH
  KPEQGSLANMPNLDSVTLKAAMVQFDRYLSAPDNLLIPQLNFLLSATVKEQIVKQSTELVCRAYGEVYAA
  VMNPINEYKDPENILHRSPQQVQTLLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020751
• ORF Size: 1971 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020751.1, NM_020751.2, NP_065802.1
• RefSeq Size: 3627 bp
• RefSeq ORF: 1974 bp
• Locus ID: 57511
• MW: 73.3 kDa
• Gene Summary: This gene encodes a subunit of the conserved oligomeric Golgi complex that is required for maintaining normal structure and activity of the Golgi apparatus. The encoded protein is organized with conserved oligomeric Golgi complex components 5, 7 and 8 into a sub-complex referred to as lobe B. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009]