DNAJC16 (NM_015291) Human Tagged ORF Clone

CAT#: RC207983

DNAJC16 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 16 (DNAJC16)

Product Images

Western blot validation of overexpression lysate (Cat# LY414676) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207983 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DNAJC16
• Synonyms: RP4-680D5.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207983 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTGAGAAAGTTGAGCATTTCCTGGCAGTTCTTGATAGTTCTGGTTCTGATCCTGCAAATTCTGT
  CTGCGTTGGATTTTGACCCATACAGAGTCCTAGGGGTCAGCCGAACAGCCAGTCAGGCTGATATTAAAAA
  GGCTTATAAGAAGCTCGCCCGGGAATGGCATCCTGACAAAAACAAAGATCCTGGAGCAGAAGACAAGTTC
  ATTCAAATCAGTAAGGCTTACGAGATTCTTTCAAATGAAGAAAAGAGATCAAATTATGATCAATATGGAG
  ACGCTGGAGAGAACCAGGGCTACCAGAAGCAGCAACAGCAGCGAGAGTATCGCTTCCGCCATTTCCATGA
  AAATTTTTATTTTGATGAATCCTTTTTTCACTTCCCTTTTAATTCTGAACGGCGGGACTCAATTGACGAA
  AAGTATTTATTGCACTTTTCACATTATGTGAATGAAGTGGTTCCAGATAGCTTCAAGAAACCCTACCTCA
  TCAAGATCACCTCCGATTGGTGCTTTAGCTGCATTCATATCGAGCCTGTGTGGAAAGAAGTCATTCAAGA
  ACTGGAAGAATTGGGTGTAGGAATTGGCGTGGTCCATGCTGGGTATGAGAGACGCCTGGCCCATCACCTA
  GGGGCACACAGCACGCCCTCTATCCTAGGAATCATTAACGGGAAAATCTCCTTCTTCCACAATGCAGTTG
  TCCGTGAAAATCTGCGACAATTTGTAGAAAGTCTTCTTCCAGGGAACTTGGTGGAGAAAGTTACAAATAA
  AAATTACGTCAGATTCCTCTCTGGCTGGCAGCAAGAGAATAAGCCTCATGTCCTTCTGTTTGACCAAACG
  CCCATTGTGCCACTGTTATACAAGTTGACTGCCTTTGCATACAAAGATTATTTATCATTTGGATATGTAT
  ATGTGGGTTTGAGAGGGACGGAAGAGATGACAAGGCGGTACAACATCAATATCTACGCCCCTACCCTCTT
  GGTCTTTAAAGAACATATAAACAGGCCTGCCGATGTTATCCAGGCCCGAGGTATGAAGAAGCAAATCATT
  GACGACTTCATCACCCGAAACAAATATCTATTGGCAGCCAGGCTCACCAGCCAGAAGTTGTTCCATGAAC
  TCTGCCCTGTGAAACGGTCGCATCGACAGAGGAAGTACTGTGTGGTTTTATTGACTGCTGAGACTACCAA
  GTTGAGCAAACCCTTTGAGGCTTTCCTGTCCTTTGCCCTGGCAAACACTCAAGACACAGTGAGATTTGTG
  CATGTCTACAGCAATCGGCAGCAGGAGTTTGCCGACACCTTACTACCAGACAGTGAGGCGTTTCAAGGGA
  AATCAGCGGTGTCTATTTTAGAAAGGCGCAACACAGCAGGAAGGGTGGTGTATAAAACCCTGGAAGACCC
  TTGGATTGGGAGTGAGAGTGACAAATTTATCCTCTTGGGCTATCTCGACCAGCTGCGTAAAGATCCAGCT
  CTTCTGTCCTCTGAAGCAGTGCTTCCTGACCTGACCGATGAACTTGCCCCTGTTTTTCTCCTTCGATGGT
  TCTACTCTGCTTCTGACTACATCTCAGACTGCTGGGATAGCATTTTTCACAACAACTGGAGGGAAATGAT
  GCCCCTGCTGTCCCTGATCTTCTCTGCCCTCTTCATCCTCTTCGGCACTGTCATCGTTCAGGCTTTCAGC
  GACTCTAATGATGAGCGAGAGTCAAGCCCTCCAGAAAAAGAGGAAGCCCAAGAGAAGACTGGGAAAACTG
  AGCCAAGCTTCACCAAAGAAAACAGCAGCAAGATTCCTAAAAAAGGCTTTGTGGAGGTAACTGAACTCAC
  AGATGTAACATACACCAGTAACTTGGTACGTCTGAGGCCAGGCCACATGAATGTGGTCCTCATCCTGTCG
  AATTCTACCAAGACCAGCCTACTACAGAAATTTGCTTTGGAGGTCTACACATTTACTGGGAGCAGCTGCC
  TACACTTCTCCTTCCTGAGTCTAGATAAACACAGAGAATGGCTAGAATACTTACTAGAATTTGCTCAAGA
  TGCAGCTCCAATCCCAAACCAATATGATAAGCATTTCATGGAGCGTGACTACACTGGTTATGTACTGGCT
  CTGAATGGCCACAAGAAATACTTCTGCCTCTTCAAGCCCCAAAAGACAGTCGAAGAGGAGGAAGCCATAG
  GGTCGTGCAGTGATGTTGACTCTTCCCTCTACCTGGGTGAATCTCGAGGGAAACCTTCCTGTGGCCTTGG
  ATCCAGGCCCATCAAAGGAAAGTTGAGCAAGCTCTCTTTATGGATGGAACGCCTGCTGGAGGGCTCCTTA
  CAGAGGTTTTATATCCCATCATGGCCTGAACTAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207983 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEVRKLSISWQFLIVLVLILQILSALDFDPYRVLGVSRTASQADIKKAYKKLAREWHPDKNKDPGAEDKF
  IQISKAYEILSNEEKRSNYDQYGDAGENQGYQKQQQQREYRFRHFHENFYFDESFFHFPFNSERRDSIDE
  KYLLHFSHYVNEVVPDSFKKPYLIKITSDWCFSCIHIEPVWKEVIQELEELGVGIGVVHAGYERRLAHHL
  GAHSTPSILGIINGKISFFHNAVVRENLRQFVESLLPGNLVEKVTNKNYVRFLSGWQQENKPHVLLFDQT
  PIVPLLYKLTAFAYKDYLSFGYVYVGLRGTEEMTRRYNINIYAPTLLVFKEHINRPADVIQARGMKKQII
  DDFITRNKYLLAARLTSQKLFHELCPVKRSHRQRKYCVVLLTAETTKLSKPFEAFLSFALANTQDTVRFV
  HVYSNRQQEFADTLLPDSEAFQGKSAVSILERRNTAGRVVYKTLEDPWIGSESDKFILLGYLDQLRKDPA
  LLSSEAVLPDLTDELAPVFLLRWFYSASDYISDCWDSIFHNNWREMMPLLSLIFSALFILFGTVIVQAFS
  DSNDERESSPPEKEEAQEKTGKTEPSFTKENSSKIPKKGFVEVTELTDVTYTSNLVRLRPGHMNVVLILS
  NSTKTSLLQKFALEVYTFTGSSCLHFSFLSLDKHREWLEYLLEFAQDAAPIPNQYDKHFMERDYTGYVLA
  LNGHKKYFCLFKPQKTVEEEEAIGSCSDVDSSLYLGESRGKPSCGLGSRPIKGKLSKLSLWMERLLEGSL
  QRFYIPSWPELD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015291
• ORF Size: 2346 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015291.1, NM_015291.2, NM_015291.3, NP_056106.1
• RefSeq Size: 6078 bp
• RefSeq ORF: 2349 bp
• Locus ID: 23341
• Protein Families: Druggable Genome, Transmembrane
• MW: 90.6 kDa