HIRA (NM_003325) Human Tagged ORF Clone

CAT#: RC208049

HIRA (Myc-DDK-tagged)-Human HIR histone cell cycle regulation defective homolog A (S. cerevisiae) (HIRA)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HIRA
• Synonyms: DGCR1; TUP1; TUPLE1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208049 representing NM_003325
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTCCTGAAGCCGACCTGGGTCAACCACAATGGCAAGCCGATTTTTTCAGTTGATATTCACCCTG
  ACGGGACCAAGTTCGCAACTGGAGGACAAGGGCAGGATTCTGGGAAGGTTGTGATCTGGAATATGTCTCC
  AGTCCTCCAGGAGGATGACGAGAAGGATGAAAATATTCCCAAGATGCTTTGCCAGATGGACAATCACTTA
  GCATGTGTGAACTGTGTGCGGTGGTCAAACAGTGGGATGTATTTAGCTTCTGGGGGAGATGACAAACTGA
  TTATGGTGTGGAAGCGGGCTACGTACATCGGCCCCAGCACCGTGTTCGGCTCCAGTGGTAAGCTTGCCAA
  TGTGGAGCAGTGGCGGTGTGTCTCTATCCTCCGGAATCATTCAGGCGATGTGATGGATGTAGCATGGTCT
  CCCCACGATGCCTGGCTAGCCTCATGCAGCGTGGATAACACTGTCGTCATCTGGAATGCTGTAAAGTTCC
  CAGAAATTCTAGCTACTCTGAGAGGTCATTCTGGCTTGGTCAAAGGGTTGACATGGGACCCTGTTGGTAA
  ATACATAGCTTCTCAAGCTGATGACCGCAGCCTAAAGGTGTGGAGGACGCTGGACTGGCAGTTGGAGACC
  AGCATCACCAAGCCTTTTGATGAGTGTGGAGGAACGACCCATGTGTTGCGGCTCAGCTGGTCACCTGATG
  GGCATTACCTGGTGTCTGCCCATGCCATGAACAACTCAGGCCCCACTGCCCAGATCATCGAACGGGAGGG
  ATGGAAGACCAACATGGACTTTGTTGGGCACCGGAAAGCTGTGACTGTCGTGAAATTCAACCCAAAAATC
  TTCAAAAAGAAGCAGAAGAATGGGAGTTCTGCGAAGCCTAGCTGCCCGTACTGCTGCTGTGCTGTTGGCA
  GCAAGGACCGCTCGCTTTCTGTCTGGCTCACATGTCTGAAACGGCCGCTGGTGGTCATCCATGAACTGTT
  TGACAAATCCATCATGGATATTTCCTGGACTCTGAATGGGCTGGGCATCTTGGTATGCTCTATGGACGGC
  TCTGTGGCATTCCTCGACTTCTCCCAGGATGAGCTTGGCGATCCCCTGAGCGAGGAGGAGAAGAGCCGCA
  TTCACCAGTCCACCTATGGCAAGAGCCTAGCCATCATGACCGAGGCCCAGCTCTCCACAGCCGTCATTGA
  GAACCCTGAGATGCTCAAGTACCAGCGAAGGCAGCAGCAGCAGCAGCTGGACCAGAAGAGTGCTGCGACC
  AGGGAGATGGGCTCAGCCACCTCAGTCGCAGGCGTTGTCAACGGGGAGAGTCTTGAAGATATCAGGAAGA
  ATCTTTTGAAGAAACAAGTTGAGACTCGGACAGCAGATGGCCGGAGAAGAATCACGCCTCTCTGCATAGC
  ACAGCTGGACACTGGGGACTTCTCCACGGCATTCTTTAACAGCATCCCCCTCTCGGGCTCCCTGGCGGGC
  ACCATGCTCTCTTCTCATAGCAGTCCACAGCTACTGCCACTGGACTCCAGTACCCCTAACTCCTTCGGCG
  CCTCGAAGCCTTGCACAGAGCCTGTGGTGGCTGCCAGTGCCAGACCTGCAGGCGATTCTGTCAATAAAGA
  CAGTATGAATGCTACCTCTACTCCTGCTGCATTGTCACCTTCTGTGTTAACGACCCCGTCCAAGATCGAA
  CCCATGAAAGCGTTTGACTCCCGGTTCACAGAGCGGTCCAAAGCCACACCAGGTGCTCCTGCCCTGACCA
  GCATGACTCCGACAGCTGTGGAAAGGTTAAAAGAGCAGAACCTTGTGAAAGAGCTGAGGCCCCGAGACCT
  CCTGGAGAGCAGCAGTGACAGCGATGAGAAAGTCCCTTTGGCTAAGGCTTCCTCACTGTCCAAGCGAAAA
  CTTGAGCTTGAGGTAGAGACAGTAGAGAAGAAGAAGAAAGGGCGGCCTCGGAAGGACTCTCGTCTCATGC
  CTGTGTCTCTGTCTGTCCAGTCTCCAGCTGCCCTAACCGCAGAGAAGGAGGCCATGTGTCTGTCTGCACC
  AGCACTTGCACTGAAGCTGCCAATTCCAAGCCCCCAGAGAGCATTCACCCTCCAGGTCAGCTCCGATCCT
  TCCATGTACATTGAGGTGGAGAATGAAGTGACAGTGGTGGGGGGCGTGAAGCTGAGCCGCCTGAAGTGCA
  ACCGGGAAGGGAAGGAGTGGGAGACGGTACTCACCAGCCGGATCCTCACTGCTGCGGGCAGCTGTGACGT
  GGTGTGTGTCGCCTGTGAAAAAAGGATGCTGTCAGTGTTCTCCACCTGTGGTCGCCGTCTCCTCTCTCCC
  ATCCTCCTGCCATCCCCGATCTCTACTTTGCATTGCACAGGCTCCTACGTCATGGCGCTCACCGCTGCAG
  CCACACTCTCTGTCTGGGATGTTCACAGACAGGTGGTTGTGGTGAAAGAAGAGTCTCTACACTCCATCCT
  GGCAGGAAGTGATATGACGGTATCACAGATCTTGCTGACGCAGCATGGAATCCCAGTAATGAACCTGTCC
  GATGGGAAGGCGTACTGCTTTAATCCGTCACTTTCCACATGGAACCTGGTTTCTGACAAGCAGGACTCAC
  TGGCTCAGTGTGCAGACTTTAGGAGCAGCCTGCCATCCCAGGACGCCATGCTGTGCTCAGGACCGTTAGC
  CATAATCCAGGGCCGCACCTCCAACTCGGGAAGGCAGGCTGCCCGGCTCTTCTCCGTGCCTCATGTGGTG
  CAGCAAGAGACCACCCTGGCCTACCTAGAGAACCAGGTGGCAGCAGCACTCACCCTGCAGTCCAGCCACG
  AGTACCGCCATTGGCTCCTCGTCTACGCACGGTACCTCGTAAACGAAGGGTTTGAATACCGACTTCGAGA
  AATATGCAAGGACTTACTGGGTCCGGTTCACTACTCCACTGGAAGCCAGTGGGAGTCAACAGTAGTGGGT
  CTGCGGAAGAGGGAGCTGCTGAAGGAGCTGCTACCAGTCATCGGGCAGAACCTCCGATTCCAGCGCCTCT
  TCACCGAGTGTCAGGAACAGCTCGACATCCTGAGGGACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208049 representing NM_003325
  Red=Cloning site Green=Tags(s)
  
  MKLLKPTWVNHNGKPIFSVDIHPDGTKFATGGQGQDSGKVVIWNMSPVLQEDDEKDENIPKMLCQMDNHL
  ACVNCVRWSNSGMYLASGGDDKLIMVWKRATYIGPSTVFGSSGKLANVEQWRCVSILRNHSGDVMDVAWS
  PHDAWLASCSVDNTVVIWNAVKFPEILATLRGHSGLVKGLTWDPVGKYIASQADDRSLKVWRTLDWQLET
  SITKPFDECGGTTHVLRLSWSPDGHYLVSAHAMNNSGPTAQIIEREGWKTNMDFVGHRKAVTVVKFNPKI
  FKKKQKNGSSAKPSCPYCCCAVGSKDRSLSVWLTCLKRPLVVIHELFDKSIMDISWTLNGLGILVCSMDG
  SVAFLDFSQDELGDPLSEEEKSRIHQSTYGKSLAIMTEAQLSTAVIENPEMLKYQRRQQQQQLDQKSAAT
  REMGSATSVAGVVNGESLEDIRKNLLKKQVETRTADGRRRITPLCIAQLDTGDFSTAFFNSIPLSGSLAG
  TMLSSHSSPQLLPLDSSTPNSFGASKPCTEPVVAASARPAGDSVNKDSMNATSTPAALSPSVLTTPSKIE
  PMKAFDSRFTERSKATPGAPALTSMTPTAVERLKEQNLVKELRPRDLLESSSDSDEKVPLAKASSLSKRK
  LELEVETVEKKKKGRPRKDSRLMPVSLSVQSPAALTAEKEAMCLSAPALALKLPIPSPQRAFTLQVSSDP
  SMYIEVENEVTVVGGVKLSRLKCNREGKEWETVLTSRILTAAGSCDVVCVACEKRMLSVFSTCGRRLLSP
  ILLPSPISTLHCTGSYVMALTAAATLSVWDVHRQVVVVKEESLHSILAGSDMTVSQILLTQHGIPVMNLS
  DGKAYCFNPSLSTWNLVSDKQDSLAQCADFRSSLPSQDAMLCSGPLAIIQGRTSNSGRQAARLFSVPHVV
  QQETTLAYLENQVAAALTLQSSHEYRHWLLVYARYLVNEGFEYRLREICKDLLGPVHYSTGSQWESTVVG
  LRKRELLKELLPVIGQNLRFQRLFTECQEQLDILRDK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003325
• ORF Size: 3051 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003325.2, NM_003325.3, NP_003316.3
• RefSeq Size: 4013 bp
• RefSeq ORF: 3054 bp
• Locus ID: 7290
• Cytogenetics: 22q11.21
• Domains: WD40
• Protein Families: Transcription Factors
• MW: 111.7 kDa
• Gene Summary: 'This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq, Jul 2008]'