EMID1 (NM_133455) Human Tagged ORF Clone

CAT#: RC208157

EMID1 (Myc-DDK-tagged)-Human EMI domain containing 1 (EMID1)

Product Images

Western blot validation of overexpression lysate (Cat# LY408793) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208157 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EMID1
• Synonyms: EMI5; EMU1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208157 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGGCCCGCGGGCTTGGGCGCTGCTCTGCCTCGGGCTCCTGCTCCCGGGAGGCGGCGCTGCGTGGA
  GCATCGGGGCAGCTCCGTTCTCCGGACGCAGGAACTGGTGCTCCTATGTGGTGACCCGCACCATCTCATG
  CCATGTGCAGAATGGCACCTACCTTCAGCGAGTGCTGCAGAACTGCCCCTGGCCCATGAGCTGTCCGGGG
  AGCAGCTACAGAACTGTGGTGAGACCCACATACAAGGTGATGTACAAGATAGTGACCGCCCGTGAGTGGA
  GGTGCTGCCCTGGGCACTCAGGAGTGAGCTGCGAGGAAGTTGCAGGTTCCTCTGCCTCCTTGGAGCCCAT
  GTGGTCGGGCAGTACCATGCGGCGGATGGCGCTTCGGCCCACAGCCTTCTCAGGTTGTCTCAACTGCAGC
  AAAGTGTCAGAGCTGACAGAGCGGCTGAAGGTGCTGGAGGCCAAGATGACCATGCTGACTGTCATAGAGC
  AGCCAGTACCTCCAACACCAGCTACCCCTGAGGACCCTGCCCCGCTCTGGGGTCCCCCTCCTGCCCAGGG
  CAGCCCCGGAGATGGAGGCCTCCAGGACCAAGTCGGTGCTTGGGGGCTTCCCGGGCCCACCGGCCCCAAG
  GGAGATGCCGGCAGTCGGGGCCCAATGGGGATGAGAGGCCCACCAGGTCCACAGGGCCCCCCAGGGAGCC
  CTGGCCGGGCTGGAGCTGTGGGCACCCCTGGAGAGAGGGGACCTCCTGGGCCACCAGGGCCTCCTGGCCC
  CCCTGGGCCCCCAGCCCCTGTTGGGCCACCCCATGCCCGGATCTCCCAGCATGGAGACCCATTGCTGTCC
  AACACCTTCACTGAGACCAACAACCACTGGCCCCAGGGACCCACTGGGCCTCCAGGCCCTCCAGGGCCCA
  TGGGTCCCCCTGGGCCTCCTGGCCCCACAGGTGTCCCTGGGAGTCCTGGTCACATAGGACCCCCAGGCCC
  CACTGGACCCAAAGGAATCTCTGGCCACCCAGGAGAGAAGGGCGAGAGAGGACTGCGTGGGGAGCCTGGC
  CCCCAAGGCTCTGCTGGGCAGCGGGGGGAACCTGGCCCTAAGGGAGACCCTGGTGAGAAGAGCCACTGGG
  GGGAGGGGTTGCACCAGCTACGCGAGGCTTTGAAGATTTTAGCTGAGAGGGTTTTAATCTTGGAAACAAT
  GATTGGGCTCTATGAGCCAGAGCTGGGGTCTGGGGCGGGCCCTGCCGGCACAGGCACCCCCAGCCTCCTT
  CGGGGCAAGAGGGGCGGACATGCAACCAACTACCGGATCGTGGCCCCCAGGAGCCGGGACGAGAGAGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208157 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGGPRAWALLCLGLLLPGGGAAWSIGAAPFSGRRNWCSYVVTRTISCHVQNGTYLQRVLQNCPWPMSCPG
  SSYRTVVRPTYKVMYKIVTAREWRCCPGHSGVSCEEVAGSSASLEPMWSGSTMRRMALRPTAFSGCLNCS
  KVSELTERLKVLEAKMTMLTVIEQPVPPTPATPEDPAPLWGPPPAQGSPGDGGLQDQVGAWGLPGPTGPK
  GDAGSRGPMGMRGPPGPQGPPGSPGRAGAVGTPGERGPPGPPGPPGPPGPPAPVGPPHARISQHGDPLLS
  NTFTETNNHWPQGPTGPPGPPGPMGPPGPPGPTGVPGSPGHIGPPGPTGPKGISGHPGEKGERGLRGEPG
  PQGSAGQRGEPGPKGDPGEKSHWGEGLHQLREALKILAERVLILETMIGLYEPELGSGAGPAGTGTPSLL
  RGKRGGHATNYRIVAPRSRDERG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133455
• ORF Size: 1329 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133455.1, NM_133455.2, NM_133455.3, NP_597712.2
• RefSeq Size: 2133 bp
• RefSeq ORF: 1332 bp
• Locus ID: 129080
• Protein Families: Secreted Protein, Transmembrane
• MW: 45.4 kDa