MGC50722 (NM_203348) Human Tagged ORF Clone

CAT#: RC208164

MGC50722 (Myc-DDK-tagged)-Human hypothetical MGC50722 (MGC50722)

Product Images

Western blot validation of overexpression lysate (Cat# LY404407) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208164 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MGC50722
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208164 representing NM_203348
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGACCCTGGTCGTGGGCACGCCGCCCACCTGCCTGGGGGACACACCTCAGCCCTGCCACAAGAACA
  GCCAGAGGCAGGGCCCCTTCTCCCATGGGGCCCCAGGGAGAGCAGCCGACTGGAAGGCTGTTGCCAAGCC
  CAGGCTCTGCGCACCTGCAGCTGAGGATGACGTGGCAGCCCTGAGGTGGCCCGGGCCCTCCCAGCAGCCA
  GACCCACCCTGGGCAGCTCCCCACGTGGTCGGGTCTGACGACCTCAAGGAACCAGGCCCCTGGGGGAAGG
  CGTGCAGCCTGCCCATGTGGTCCACAGGCCCGGAGGCTAGGGATGGGGACAGCTCGGTGTCATCGGGCCG
  CCTCTCGTGCTCTTCGGGGGGCCACGACGTGTGTGTGTCTTGGAAGGAGAGGCCACCCCAGGTGTTGGGG
  CCCCAGCAGAGGCCCAGAAAGAGTGACGCGCGGCTGGAGCAGCTGAGAGACAAGATCCGGGCCCAGGCGT
  GGCAGCAGGGGAGCTGTGCGTCCCTGGGCACCTCAGCCCCCTCCAGCGCCTCCAGACTCCACAAAGCCTC
  CACGCTGACGCTTAGGAGGAAAGGCCAAGAGGCAAAAAATCCCCCTCCAGCCCCTGAATGCTCAGGTTTC
  AGCATCTTGAGTGCAGCTGAGCGCAGAGTTGAAGCCAAGGCATCCCACGGCCAGGGGCGCGAGCTCTCCA
  GGGTCTCCCAGCACCAGGTTCCTGTTCTGAGGGAAAAACCCAAAAGGGTCAAAAGCAGTTCTTGCAAAAG
  AGAGAAGACCCCCAAGTTGCCCTCCCCTAGAAGAGCGGCCAAAGACAAACACAAAGACGAAGATTCGGAG
  CTGGTTGGTGTTTACGCCTGGAGAAAGGGACAAGCTCTGGTGAGGTCGCTGCTTGGGCCCCCTCCCGTCC
  TCCGCAGGCACCATAGCAAGGATCCCTCGAGAGACCCGGCTCTCACCGTGGACTTAGGAGACAGCGAGAA
  AGTCATAGCTGCCAAGTGCTCACCGGTTTGTGCCCAGTTGCCCGATGCCACCTCCGCCCACAGTGACCAG
  CAGGTGTCTGGGAACACACCCAGCCTGGCTTCCTTCGACCAGCCAGCGACCATACAGACGGCCATGGCCA
  TCCTACAAGACCTGCGCCAGCAAATCCAGGCTGGGCTGGAGCTGGCCCAGGCCCGCAAGGGAGGGCAAGA
  GCTCGGGCCATCAAAGCGGAGGCTGCAGGACGTGGCAGGGAGGGGCCGCTGCAGGGACCCCAATGCCCAG
  AGCTCCTTCTCTAAGAGTCCCTGGGCCATGACAGAGAGGAAGCATTCCTCTTTAGAGAGGGCTAGGAGTG
  TCCACACCTGGGAGCCCTGGAGCTCCTCCACTGCACGGGAGTCCTGTCCGCAGAGGGCCTGGGGGGCCCA
  AGGACAGGACCGCTCCTTCCAGAGGCCGGAGAGTCCCCATGAGAGGTTGGGCCACTTCTCCCAGAGGCCC
  TGGAGTGCACTGGCTGGGCAGGCCTGCAGTCCGCAGAGGGCCTGGGGGGCCCAAAGACAGGGCCCCTCCT
  CCCAGAGGCCTGGGAGCCCCCCTGAAAAGCGGAGCCCCTTCCCCCAGCAGCCCTGGAGTGCTGTAGCCAC
  ACAGCCCTGTCCACGGAGGGCCTGGACTGCCTGTGAAACCTGGGAGGACCCTGGCCCCAGACTGCGGAAC
  CCTCTGGAAAGGCCCAGTCCTCCAGCCCAGCGGCCCTGGAGCAGCTCCGGCGTGCAGAGGGCCGGCCCCC
  AGGGCAAGGGCAGAGGCATCGGCTCCCCCGTCTCGGCTGCCAAGCATGCCCTGCCAAGGCCCACCGGGAG
  CTTCCCTCAGAACCCACTTGGGAAGGAGAAGGACACGCTGCGGCCCTGCCCCAGGTCCCGGGGGCTCCTG
  GGACCCTCGCACAGCTCTGAGTCCTTGCGGGAGTTCATGCGCCAGAAGGCGCAGGCCCGGCGGCGGCAGG
  CCCTGGAGGAGAAGGCCTCGGCCCTGCGCACACGGGAGCTGAGGAGCCGGAGGCTGCAGGAGGTCTACCG
  GCAGCAGAGGGAGGCCGTCCTCGGCAGGGCGGTCCCCGTGGTCTCCCGGACCACCCCTGGCATCGTGACC
  TTTGTCCCCAGTTCTGCACAGTCCGGGGGCCTGGAAGCCTCCGGGAGCCTGGAGTCCCCAGTGCTGGAAT
  GGAGCAAAGTGACCTCTGGCATGGTGCTGGGGGGCCAGGAAGCCCCAGGCAGCTTCTGCCTGTGTTTGAA
  CAGAGCCTGGAACCATGCTGAGACCCTAGATCCCCCAGGGATGGGGGGCCCCCAAGATGGCCGGGATGCC
  CCCGTGCTGCTGTCAGCTTCACCCTCTCTGGGATCCCTGGAGCTCCAGGACCTGACCACCCGCTACCTAC
  CCCGGGGGATGTGCATCTACCTGGACCCGAAGGAGGCTGAGCACCTGGGCACTTCCAGCTCCCTGCACCT
  CCGGCATAAGCAGGCGCAGCTGCAGGCGCTAGAGACCACAGCCAAAGTCCTCAAGCAGCGGGTCGATAGC
  CTCACCGCCAAGCTTCAGGGTGCCGAAGCGCCGGACACCGTCAGGGACCCAGCTGTGGGCCTGCTGCGCT
  CGTGCCCCCACAGCCTACCTGCCGCCCCCACGCTCGCCACCCCCACGCTTGCCACCCCAGCCTGCCCTGG
  AGCCTTGGGGCCCAACTGGGGCAGAGGGGCACCTGGGGAGTGGGTGAGCATGCAGCCCCAGCCCCTTCTG
  CCCCCGACCTACTTCCTGGAGGGCGAGACACTGTCGTGGGGCCCCAGCTGGGAGCAACAGCAGAGTGTGA
  GCCCGAGGGCCCACTGCGAGAGCAAGCCCCGAGGTGTGTCTCAGGCTCCTCTCCTGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208164 representing NM_203348
  Red=Cloning site Green=Tags(s)
  
  MPTLVVGTPPTCLGDTPQPCHKNSQRQGPFSHGAPGRAADWKAVAKPRLCAPAAEDDVAALRWPGPSQQP
  DPPWAAPHVVGSDDLKEPGPWGKACSLPMWSTGPEARDGDSSVSSGRLSCSSGGHDVCVSWKERPPQVLG
  PQQRPRKSDARLEQLRDKIRAQAWQQGSCASLGTSAPSSASRLHKASTLTLRRKGQEAKNPPPAPECSGF
  SILSAAERRVEAKASHGQGRELSRVSQHQVPVLREKPKRVKSSSCKREKTPKLPSPRRAAKDKHKDEDSE
  LVGVYAWRKGQALVRSLLGPPPVLRRHHSKDPSRDPALTVDLGDSEKVIAAKCSPVCAQLPDATSAHSDQ
  QVSGNTPSLASFDQPATIQTAMAILQDLRQQIQAGLELAQARKGGQELGPSKRRLQDVAGRGRCRDPNAQ
  SSFSKSPWAMTERKHSSLERARSVHTWEPWSSSTARESCPQRAWGAQGQDRSFQRPESPHERLGHFSQRP
  WSALAGQACSPQRAWGAQRQGPSSQRPGSPPEKRSPFPQQPWSAVATQPCPRRAWTACETWEDPGPRLRN
  PLERPSPPAQRPWSSSGVQRAGPQGKGRGIGSPVSAAKHALPRPTGSFPQNPLGKEKDTLRPCPRSRGLL
  GPSHSSESLREFMRQKAQARRRQALEEKASALRTRELRSRRLQEVYRQQREAVLGRAVPVVSRTTPGIVT
  FVPSSAQSGGLEASGSLESPVLEWSKVTSGMVLGGQEAPGSFCLCLNRAWNHAETLDPPGMGGPQDGRDA
  PVLLSASPSLGSLELQDLTTRYLPRGMCIYLDPKEAEHLGTSSSLHLRHKQAQLQALETTAKVLKQRVDS
  LTAKLQGAEAPDTVRDPAVGLLRSCPHSLPAAPTLATPTLATPACPGALGPNWGRGAPGEWVSMQPQPLL
  PPTYFLEGETLSWGPSWEQQQSVSPRAHCESKPRGVSQAPLLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203348
• ORF Size: 2859 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_203348.1, NP_976223.1
• RefSeq Size: 3630
• RefSeq ORF: 2861
• Locus ID: 399693
• MW: 102.9 kDa