NCAM2 (NM_004540) Human Tagged ORF Clone

CAT#: RC208203

NCAM2 (Myc-DDK-tagged)-Human neural cell adhesion molecule 2 (NCAM2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NCAM2
• Synonyms: NCAM21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208203 representing NM_004540
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCTCCTCCTCTCCTTCTACCTGCTGGGGTTGCTTGTCAGTAGCGGGCAAGCTCTTCTTCAAGTGA
  CAATTTCACTTAGCAAAGTAGAGCTTAGTGTTGGAGAATCTAAATTCTTCACATGTACAGCGATTGGTGA
  ACCTGAAAGTATAGATTGGTATAATCCTCAAGGAGAGAAGATAATTTCAACACAGAGGGTAGTAGTGCAA
  AAGGAAGGTGTTAGGTCACGGTTAACCATCTACAATGCAAATATAGAAGATGCAGGGATATATCGTTGTC
  AAGCAACAGATGCCAAAGGACAAACACAAGAAGCTACAGTAGTTTTGGAAATTTACCAAAAACTCACTTT
  CAGAGAAGTGGTATCTCCACAAGAATTCAAACAAGGAGAAGATGCAGAAGTGGTTTGCCGAGTTAGCAGT
  TCACCTGCACCTGCTGTCAGCTGGTTGTATCATAATGAGGAAGTCACCACTATTTCCGACAATCGGTTCG
  CTATGTTAGCAAACAATAACCTGCAGATTCTCAACATCAATAAAAGTGATGAAGGTATATACAGATGTGA
  AGGAAGAGTGGAGGCCAGGGGAGAAATTGACTTCCGTGATATCATTGTTATTGTTAATGTGCCGCCAGCA
  ATCTCAATGCCTCAGAAATCTTTTAATGCCACAGCAGAGAGAGGAGAAGAAATGACATTTTCCTGCAGGG
  CCTCAGGCTCTCCAGAACCCGCCATCTCCTGGTTCAGGAATGGCAAGCTCATTGAAGAAAATGAGAAGTA
  CATATTGAAAGGGAGCAATACAGAACTCACTGTCAGGAACATAATCAATAGTGATGGTGGTCCTTATGTC
  TGCAGGGCCACAAATAAGGCAGGAGAAGATGAAAAGCAAGCTTTCCTCCAAGTCTTTGTACAGCCTCACA
  TAATACAGCTTAAAAATGAAACTACATATGAGAATGGTCAAGTCACACTCGTATGTGATGCGGAAGGGGA
  GCCTATTCCAGAAATCACTTGGAAAAGAGCTGTGGATGGCTTCACGTTCACTGAAGGCGATAAGAGCCTG
  GACGGCCGTATCGAAGTCAAAGGGCAGCATGGAAGCTCATCACTGCATATTAAAGATGTGAAGTTGTCAG
  ATTCAGGGAGATATGACTGTGAAGCTGCAAGCAGAATTGGAGGGCATCAAAAGAGCATGTACCTTGATAT
  TGAATATGCCCCCAAGTTTATATCAAACCAAACAATTTATTACTCTTGGGAAGGAAATCCTATCAATATA
  AGTTGTGATGTGAAATCGAATCCACCAGCATCAATTCACTGGAGAAGAGATAAATTAGTCTTACCTGCTA
  AAAACACGACCAATTTAAAGACTTATAGTACAGGAAGAAAGATGATATTAGAGATTGCACCTACATCTGA
  CAATGACTTTGGACGCTATAATTGCACAGCCACTAATCATATAGGAACAAGATTTCAAGAATATATTCTT
  GCTTTGGCTGACGTGCCATCCAGTCCCTATGGAGTGAAGATCATAGAGCTGTCGCAGACCACGGCCAAGG
  TTTCCTTCAACAAACCGGACTCCCATGGAGGTGTACCTATTCATCACTATCAGGTGGATGTCAAAGAAGT
  AGCGTCAGAAATCTGGAAAATTGTACGCTCCCATGGAGTTCAAACAATGGTTGTTTTGAACAACCTGGAA
  CCAAATACAACTTATGAAATCAGGGTTGCAGCTGTAAATGGAAAGGGACAAGGAGACTACAGTAAAATAG
  AAATCTTCCAAACATTACCAGTTCGTGAACCAAGTCCTCCATCCATACATGGACAGCCAAGCAGTGGAAA
  GAGCTTTAAACTCAGCATCACCAAACAGGACGATGGAGGGGCCCCTATTTTGGAATACATTGTGAAATAT
  AGAAGTAAAGATAAGGAAGACCAATGGCTAGAGAAAAAAGTGCAAGGAAATAAAGACCACATCATTTTGG
  AGCATCTCCAGTGGACCATGGGGTATGAAGTTCAGATTACAGCTGCCAATAGATTGGGATATTCTGAACC
  GACAGTTTATGAATTCAGCATGCCACCAAAGCCCAACATTATTAAAGACACGCTGTTTAATGGTCTTGGG
  CTTGGAGCAGTAATTGGCCTGGGAGTTGCTGCACTGCTGCTAATTCTTGTGGTAACAGACGTCAGCTGCT
  TCTTTATTCGGCAATGTGGGTTGCTGATGTGCATCACTAGGAGAATGTGTGGAAAGAAAAGTGGCTCCAG
  TGGCAAAAGTAAAGAACTCGAAGAAGGAAAAGCTGCATACCTGAAAGATGGATCAAAAGAACCAATAGTG
  GAGATGAGAACAGAGGATGAAAGAGTTACTAATCACGAAGATGGGAGCCCAGTAAATGAGCCAAATGAAA
  CCACACCACTGACAGAACCTGAAAAATTGCCTTTAAAGGAAGAAGATGGGAAAGAAGCTCTAAATCCAGA
  AACTATAGAAATTAAAGTTTCTAACGACATCATTCAATCAAAAGAAGACGACAGCAAAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208203 representing NM_004540
  Red=Cloning site Green=Tags(s)
  
  MSLLLSFYLLGLLVSSGQALLQVTISLSKVELSVGESKFFTCTAIGEPESIDWYNPQGEKIISTQRVVVQ
  KEGVRSRLTIYNANIEDAGIYRCQATDAKGQTQEATVVLEIYQKLTFREVVSPQEFKQGEDAEVVCRVSS
  SPAPAVSWLYHNEEVTTISDNRFAMLANNNLQILNINKSDEGIYRCEGRVEARGEIDFRDIIVIVNVPPA
  ISMPQKSFNATAERGEEMTFSCRASGSPEPAISWFRNGKLIEENEKYILKGSNTELTVRNIINSDGGPYV
  CRATNKAGEDEKQAFLQVFVQPHIIQLKNETTYENGQVTLVCDAEGEPIPEITWKRAVDGFTFTEGDKSL
  DGRIEVKGQHGSSSLHIKDVKLSDSGRYDCEAASRIGGHQKSMYLDIEYAPKFISNQTIYYSWEGNPINI
  SCDVKSNPPASIHWRRDKLVLPAKNTTNLKTYSTGRKMILEIAPTSDNDFGRYNCTATNHIGTRFQEYIL
  ALADVPSSPYGVKIIELSQTTAKVSFNKPDSHGGVPIHHYQVDVKEVASEIWKIVRSHGVQTMVVLNNLE
  PNTTYEIRVAAVNGKGQGDYSKIEIFQTLPVREPSPPSIHGQPSSGKSFKLSITKQDDGGAPILEYIVKY
  RSKDKEDQWLEKKVQGNKDHIILEHLQWTMGYEVQITAANRLGYSEPTVYEFSMPPKPNIIKDTLFNGLG
  LGAVIGLGVAALLLILVVTDVSCFFIRQCGLLMCITRRMCGKKSGSSGKSKELEEGKAAYLKDGSKEPIV
  EMRTEDERVTNHEDGSPVNEPNETTPLTEPEKLPLKEEDGKEALNPETIEIKVSNDIIQSKEDDSKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004540
• ORF Size: 2511 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004540.1, NM_004540.2, NM_004540.3, NP_004531.2
• RefSeq Size: 3699 bp
• RefSeq ORF: 2514 bp
• Locus ID: 4685
• Cytogenetics: 21q21.1
• Domains: ig, IGc2, IG, FN3
• Protein Families: Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Prion diseases
• MW: 93.05 kDa
• Gene Summary: 'The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein and may function in selective fasciculation and zone-to-zone projection of the primary olfactory axons. [provided by RefSeq, Jul 2008]'