Syntaxin 12 (STX12) (NM_177424) Human Tagged ORF Clone

CAT#: RC208282

STX12 (Myc-DDK-tagged)-Human syntaxin 12 (STX12)

Product Images

Western blot validation of overexpression lysate (Cat# LY406161) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208282 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STX12
• Synonyms: STX13; STX14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208282 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATACGGTCCCTTAGACATGTACCGGAACCCGGGGCCCTCGGGGCCCCAGCTCCGGGACTTCAGCA
  GCATCATCCAGACGTGCAGCGGCAACATCCAGCGGATCAGCCAAGCCACTGCTCAGATAAAGAATTTGAT
  GAGCCAGCTAGGAACTAAGCAGGACTCAAGCAAGCTACAGGAAAATCTGCAACAGTTACAACACTCCACA
  AATCAGCTCGCCAAGGAAACAAATGAATTGCTGAAAGAATTAGGGTCCTTGCCCCTTCCCTTATCTACTT
  CAGAACAGCGCCAGCAGAGACTTCAGAAGGAACGCCTCATGAATGACTTCTCTGCAGCCTTAAACAATTT
  CCAGGCTGTGCAGAGAAGGGTATCTGAAAAGGAAAAGGAGAGTATTGCCAGAGCAAGAGCTGGATCTCGT
  CTTTCTGCAGAAGAGAGGCAAAGAGAGGAGCAGCTGGTCTCATTTGACAGCCATGAGGAGTGGAACCAGA
  TGCAGAGCCAGGAGGATGAGGTGGCCATCACTGAGCAGGATTTGGAACTTATTAAAGAAAGAGAAACGGC
  AATTCGGCAGCTGGAGGCTGACATTTTGGATGTCAATCAGATATTTAAAGATTTGGCCATGATGATCCAT
  GACCAGGGTGATCTGATTGATAGCATAGAAGCCAATGTGGAAAGCTCAGAGGTGCACGTCGAAAGAGCCA
  CTGAACAGTTACAGCGAGCTGCTTACTATCAGAAAAAATCTCGCAAGAAGATGTGTATCCTGGTGCTTGT
  CCTGTCAGTGATTATTCTAATCTTGGGACTTATTATCTGGCTAGTTTATAAAACGAAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208282 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSYGPLDMYRNPGPSGPQLRDFSSIIQTCSGNIQRISQATAQIKNLMSQLGTKQDSSKLQENLQQLQHST
  NQLAKETNELLKELGSLPLPLSTSEQRQQRLQKERLMNDFSAALNNFQAVQRRVSEKEKESIARARAGSR
  LSAEERQREEQLVSFDSHEEWNQMQSQEDEVAITEQDLELIKERETAIRQLEADILDVNQIFKDLAMMIH
  DQGDLIDSIEANVESSEVHVERATEQLQRAAYYQKKSRKKMCILVLVLSVIILILGLIIWLVYKTK
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177424
• ORF Size: 828 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177424.1, NM_177424.2, NP_803173.1
• RefSeq Size: 3098 bp
• RefSeq ORF: 831 bp
• Locus ID: 23673
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• MW: 31.6 kDa