POLR3B (NM_018082) Human Tagged ORF Clone
CAT#: RC208356
- TrueORF®
POLR3B (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant 1
"NM_018082" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | POLR3B |
Synonyms | C128; HLD8; INMAP; RPC2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC208356 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTGCTAGCGGAGGAGTTTGGGAACCTGACTCCGGAGCAGCTGGCGGCGCCGATCCCGACTGTAG AGGAAAAATGGAGGCTGCTTCCAGCATTTTTAAAGGTGAAAGGCCTTGTGAAACAGCATATAGATTCATT TAACTATTTCATTAATGTAGAGATAAAGAAGATAATGAAAGCCAATGAAAAGGTTACAAGTGACGCTGAC CCTATGTGGTACTTAAAATATCTTAATATCTATGTTGGGCTTCCTGATGTTGAAGAAAGCTTCAATGTAA CTAGACCAGTGTCCCCTCATGAGTGCCGTTTGAGAGACATGACATACTCTGCCCCTATTACAGTGGATAT TGAATATACCCGAGGCAGCCAGAGGATCATCCGCAATGCCTTACCTATCGGCAGAATGCCCATAATGCTA CGTAGTTCAAACTGTGTTCTTACAGGAAAAACGCCAGCAGAATTTGCCAAACTGAACGAATGTCCCTTAG ATCCAGGTGGCTACTTCATTGTTAAAGGAGTAGAAAAAGTTATTCTTATCCAAGAGCAGCTGTCTAAGAA CAGGATCATCGTGGAGGCTGATAGAAAAGGGGCTGTTGGAGCTTCAGTTACCAGCTCTACCCATGAGAAA AAAAGCAGAACCAATATGGCTGTGAAACAAGGACGATTTTATTTGAGGCATAATACTTTGTCAGAAGATA TACCCATTGTCATCATATTTAAGGCCATGGGTGTTGAGAGTGACCAGGAAATTGTGCAGATGATTGGAAC AGAGGAGCACGTGATGGCTGCATTTGGGCCCAGTCTGGAAGAGTGCCAGAAAGCTCAGATTTTCACACAG ATGCAGGCATTAAAATATATAGGGAACAAAGTAAGAAGGCAAAGGATGTGGGGAGGTGGACCAAAGAAAA CCAAAATAGAAGAAGCAAGAGAGCTCCTGGCTTCCACCATTCTGACCCATGTCCCAGTTAAGGAATTCAA TTTCCGAGCCAAATGTATCTATACTGCAGTGATGGTGCGAAGAGTTATTCTGGCCCAAGGAGATAATAAA GTTGACGACAGAGATTATTATGGTAACAAGCGACTGGAATTGGCAGGACAGCTTTTATCTCTTCTTTTTG AAGACTTGTTCAAAAAATTTAATTCTGAAATGAAAAAGATTGCCGACCAGGTGATTCCTAAGCAAAGAGC AGCCCAGTTTGATGTTGTCAAACACATGCGCCAAGACCAGATCACCAATGGCATGGTGAATGCTATTTCT ACCGGAAATTGGTCTTTAAAGAGATTTAAAATGGACCGCCAGGGTGTAACCCAAGTGCTGTCTCGCTTGT CATATATATCCGCACTGGGCATGATGACAAGAATCTCTTCCCAGTTTGAAAAAACGAGAAAAGTGAGTGG TCCTCGCTCCCTCCAGCCATCTCAGTGGGGAATGCTGTGTCCTTCGGACACTCCTGAAGGAGAGGCATGT GGTTTGGTTAAAAACTTGGCCCTTATGACACACATCACAACTGATATGGAAGATGGACCCATTGTTAAAT TAGCCAGTAACTTGGGAGTAGAAGATGTGAATTTATTATGTGGGGAAGAGCTCTCTTACCCAAATGTGTT TCTTGTCTTTCTTAATGGTAACATCTTAGGTGTCATTCGAGACCACAAAAAGCTAGTGAATACATTTCGA CTCATGAGAAGAGCAGGATATATCAATGAATTTGTTTCCATCTCAACAAATCTTACAGATCGATGTGTCT ATATTTCTTCTGATGGGGGAAGGCTATGCAGACCCTACATAATTGTCAAGAAACAGAAGCCAGCAGTCAC AAATAAACATATGGAAGAGCTGGCCCAAGGGTACAGGAATTTTGAAGATTTCTTACATGAGAGTCTGGTT GAATATTTAGATGTGAATGAAGAAAATGATTGTAACATTGCACTGTACGAACACACAATTAATAAAGACA CCACCCACTTGGAGATTGAACCCTTCACTCTTCTCGGCGTGTGTGCTGGACTTATCCCATACCCTCACCA TAACCAGTCACCGAGAAACACTTATCAGTGTGCCATGGGGAAACAAGCCATGGGTACTATAGGATACAAC CAGCGAAACAGAATTGATACTCTCATGTATCTACTAGCATATCCACAAAAACCCATGGTTAAGACAAAAA CCATTGAATTGATAGAATTTGAGAAACTGCCAGCTGGACAGAATGCAACAGTTGCTGTGATGAGCTATAG TGGCTATGATATTGAAGATGCTCTTGTTTTAAACAAGGCCTCTTTAGACAGAGGCTTTGGGCGTTGCCTT GTATATAAAAATGCTAAATGTACGTTGAAACGATACACCAATCAGACTTTTGATAAAGTGATGGGGCCCA TGTTGGATGCTGCTACAAGGAAACCTATCTGGCGACATGAAATCTTAGATGCAGATGGTATTTGTTCTCC AGGTGAGAAAGTAGAAAACAAACAAGTGCTTGTAAATAAGTCCATGCCCACAGTGACTCAGATTCCTTTG GAAGGAAGTAATGTACCACAGCAACCACAGTACAAAGATGTACCCATAACCTACAAAGGAGCAACAGACT CATATATTGAAAAAGTGATGATATCTTCAAATGCTGAAGATGCTTTTCTGATCAAAATGCTGCTGAGACA GACAAGGCGTCCAGAAATTGGAGACAAATTCAGCAGTCGTCATGGGCAAAAAGGTGTTTGTGGCTTGATC GTCCCCCAGGAAGACATGCCATTTTGTGATTCTGGCATCTGTCCGGACATCATCATGAACCCACACGGCT TCCCATCACGAATGACGGTGGGGAAGCTCATTGAGCTGCTGGCTGGCAAGGCCGGTGTGCTGGACGGCAG ATTCCACTACGGCACTGCGTTTGGAGGCAGTAAAGTGAAGGATGTGTGTGAGGACCTCGTTCGCCATGGT TATAACTACTTGGGGAAAGACTATGTTACATCCGGCATCACAGGTGAGCCCTTAGAAGCATACATCTATT TTGGCCCCGTGTACTATCAGAAGCTGAAACACATGGTGCTAGATAAAATGCATGCCCGGGCCCGGGGCCC ACGAGCCGTCCTTACCAGGCAACCCACTGAAGGACGGTCTCGTGATGGTGGCTTGCGTCTCGGGGAAATG GAACGTGACTGTTTAATCGGTTATGGAGCCAGTATGCTTTTGCTAGAGAGACTAATGATTTCAAGTGATG CCTTTGAGGTTGATGTCTGTGGGCAGTGTGGACTTCTGGGGTATTCTGGCTGGTGCCATTACTGCAAGTC ATCCTGCCACGTGTCTTCCCTCCGTATTCCGTATGCCTGCAAGCTGCTCTTCCAGGAACTACAGTCTATG AACATCATCCCCAGGTTAAAACTGTCCAAGTACAATGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208356 protein sequence
Red=Cloning site Green=Tags(s) MDVLAEEFGNLTPEQLAAPIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDAD PMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIML RSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGAVGASVTSSTHEK KSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQ MQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNK VDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAIS TGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEAC GLVKNLALMTHITTDMEDGPIVKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVNTFR LMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLV EYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYN QRNRIDTLMYLLAYPQKPMVKTKTIELIEFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCL VYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPL EGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLI VPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHG YNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEM ERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSM NIIPRLKLSKYNE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_018082 |
ORF Size | 3399 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_018082.2, NM_018082.3, NM_018082.4, NM_018082.5, NP_060552.3 |
RefSeq Size | 4286 |
RefSeq ORF | 3402 |
Locus ID | 55703 |
Domains | RNA_pol_Rpb2_6, RNA_pol_Rpb2_7, RNA_pol_Rpb2_2, RNA_pol_Rpb2_1, RNA_pol_Rpb2_3, RNA_pol_Rpb2_4, RNA_pol_Rpb2_5 |
Protein Families | Transcription Factors |
Protein Pathways | Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
MW | 127.8 kDa |
Gene Summary | This gene encodes the second largest subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The largest subunit and the encoded protein form the catalytic center of RNA polymerase III. Mutations in this gene are a cause of hypomyelinating leukodystrophy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC113761 | POLR3B (untagged)-Human polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant 1 |
USD 310.00 |
|
RG208356 | POLR3B (GFP-tagged) - Human polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant 1 |
USD 970.00 |
|
RC208356L3 | Lenti-ORF clone of POLR3B (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant 1 |
USD 1,080.00 |
|
RC208356L4 | Lenti-ORF clone of POLR3B (mGFP-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant 1 |
USD 1,080.00 |
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